Human herpesvirus type 1 and type 2 disrupt mitochondrial dynamics in human keratinocytes.

Abstract:

:Mitochondrial movement and distribution throughout the cytoplasm is crucial for maintaining cell homeostasis. Mitochondria are dynamic organelles but can be functionally disrupted during infection. Here, we show that the ubiquitous human pathogens HHV-1 and HHV-2 induce changes in the mitochondrial morphology and distribution in the early and late phases of productive infection in human keratinocytes (HaCaT cells). We observed a decrease in the mitochondrial potential at 2 h postinfection and a decrease in cell vitality at 24 h postinfection. Moreover, we found that mitochondria migrated to the perinuclear area, where HHV-1 and HHV-2 antigens were also observed, mainly in the early stages of infection. Positive results of real-time PCR showed a high level of HHV-1 and HHV-2 DNA in HaCaT cells and culture medium. Our data demonstrate that HHV-1 and HHV-2 cause mitochondrial dysfunction in human keratinocytes.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Chodkowski M,Serafińska I,Brzezicka J,Golke A,Słońska A,Krzyżowska M,Orłowski P,Bąska P,Bańbura MW,Cymerys J

doi

10.1007/s00705-018-3890-y

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

2663-2673

issue

10

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-018-3890-y

journal_volume

163

pub_type

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