Abstract:
:Mitochondrial movement and distribution throughout the cytoplasm is crucial for maintaining cell homeostasis. Mitochondria are dynamic organelles but can be functionally disrupted during infection. Here, we show that the ubiquitous human pathogens HHV-1 and HHV-2 induce changes in the mitochondrial morphology and distribution in the early and late phases of productive infection in human keratinocytes (HaCaT cells). We observed a decrease in the mitochondrial potential at 2 h postinfection and a decrease in cell vitality at 24 h postinfection. Moreover, we found that mitochondria migrated to the perinuclear area, where HHV-1 and HHV-2 antigens were also observed, mainly in the early stages of infection. Positive results of real-time PCR showed a high level of HHV-1 and HHV-2 DNA in HaCaT cells and culture medium. Our data demonstrate that HHV-1 and HHV-2 cause mitochondrial dysfunction in human keratinocytes.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Chodkowski M,Serafińska I,Brzezicka J,Golke A,Słońska A,Krzyżowska M,Orłowski P,Bąska P,Bańbura MW,Cymerys Jdoi
10.1007/s00705-018-3890-ysubject
Has Abstractpub_date
2018-10-01 00:00:00pages
2663-2673issue
10eissn
0304-8608issn
1432-8798pii
10.1007/s00705-018-3890-yjournal_volume
163pub_type
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