Abstract：
:Chilo iridescent virus (CIV; the type strain of the genus Iridovirus) replicates productively in larvae of the boll weevil, Anthonomus grandis. This study focuses on characterizing productive infections of a boll weevil cell line, BRL-AG-3A (AG3A), starting with CIV reared in the waxworm, Galleria mellonella. We show that CIV can be continually and productively passaged to high titer in AG3A cells. The replication of larval-derived CIV in AG3A was analyzed by observing viral DNA replication and restriction endonuclease digestion profiles, morphogenesis, and infectivity using TCID(50) assays with AG3A as an indicator cell line. The data showed that virus passaged in the AG3A host is stable. AG3A cells are more efficient than previously utilized CF-124T cells from Choristoneura fumiferana. This system constitutes a superior model for cellular and molecular studies on CIV; it represents the first complete, productive cell culture model for the replication of CIV or any member of the genus Iridovirus.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
D'Costa SM,Vigerust DJ,Perales-Hull MR,Lodhi SA,Viravathana P,Bilimoria SLdoi
10.1007/s00705-012-1417-5subject
Has Abstractpub_date
2012-11-01 00:00:00pages
2171-8issue
11eissn
0304-8608issn
1432-8798journal_volume
157pub_type
杂志文章abstract：:Algeria is the largest country of Africa, with a population of 40 million inhabitants living in disparate environments from the Sahara to the large cities of the Mediterranean coast. The molecular epidemiology of hepatitis B virus (HBV) variants has been partially described, but variations in the seroprevalence of HBV...
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更新日期：1985-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314319
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pub_type： 杂志文章
doi：10.1007/s00705-004-0390-z
更新日期：2005-01-01 00:00:00
