Abstract：
:Highly purified virulent poliovirus preparations harbour an endogenous protein kinase. Enzyme activity increases significantly upon purification of infectious virus particles from infected HeLa cells. Enzyme activity is stimulated by divalent cations. The substrate specificity and the degree of stimulation of the kinase are dependent on the nature of the divalent cations included in the assay. The preferred substrates for this kinase are the viral capsid proteins. Exogenously added proteins such as alpha-casein, phosvitin and protamine are also phosphorylated by the kinase. Moreover, these proteins enhance the phosphorylation of viral proteins. In the presence of Mg++ VP 2 and VP 0 are highly phosphorylated, while in the presence of Zn++ only VP 2 and VP 4, but not VP 0 or exogenous proteins are phosphorylated. Poliovirus associated protein kinase exhibits optimal activity at pH 7.9 in the presence of 10 mM Mg++. The Km for ATP is shown to be 40 microM. By testing different nucleotides as phosphate donors a specificity of the phosphorylation reaction for ATP is demonstrated. Phosphoamino acid analysis of hydrolysates of the substrates phosphorylated in the presence of Mg++ by thin layer electrochromatography and HPLC yielded phosphoserine and phosphothreonine from viral capsid proteins while hydrolysates of protamine yield only phosphoserine. Destabilization of the viral capsid, e.g. by preincubation at 42 degrees C for 20 minutes results in a stimulation of kinase activity. Moreover, phosphorylation of the poliovirus capsid proteins itself results in destabilization of the viral capsid. These findings suggest that phosphorylation of the viral coat proteins triggers or enhances the uncoating of poliovirus leading to the release of viral RNA.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Lackmann M,Ueckermann C,Engelmann K,Koch Gdoi
10.1007/BF01311330subject
Has Abstractpub_date
1987-01-01 00:00:00pages
1-16issue
1-2eissn
0304-8608issn
1432-8798journal_volume
95pub_type
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journal_title：Archives of virology
pub_type： 杂志文章
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journal_title：Archives of virology
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更新日期：1993-01-01 00:00:00
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doi：10.1007/BF01718833
更新日期：1996-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF01315679
更新日期：1978-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF01319816
更新日期：1988-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：1979-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315054
更新日期：1977-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF01310963
更新日期：1985-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3192-1
更新日期：2017-04-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309681
更新日期：1992-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF01321010
更新日期：1988-01-01 00:00:00
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更新日期：2007-01-01 00:00:00
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更新日期：2017-04-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311162
更新日期：1984-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：2014-12-01 00:00:00
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更新日期：2019-05-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：1981-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s007050050210
更新日期：1997-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2018-05-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：2016-08-01 00:00:00
