Abstract：
:Infectious cDNA clones of the Shanghai isolate of Ribgrass mosaic virus (RMV) were produced by joining four overlapping cDNA fragments and also in a single step by long template PCR. After inoculation of Nicotiana glutinosa with either RNA transcripts or the cDNA under the control of the CaMV 35S promoter, plants developed typical symptoms, and viral coat protein could be detected in them by Western blot analysis. However, compared to plants inoculated with purified viral RNA, lesions were fewer and appeared more slowly. An epitope of the Mycobacterium tuberculosis 31-kDa protein was inserted at the C-terminus of the viral coat protein by PCR using two overlapping fragments. The modified clone was also infectious and the foreign epitope could be detected serologically in the electron microscope and by Western blot analysis. The results demonstrate the potential of RMV as a viral gene vector.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Zhu HQ,Chen JP,Yu SQdoi
10.1007/s00705-006-0740-0subject
Has Abstractpub_date
2006-09-01 00:00:00pages
1853-61issue
9eissn
0304-8608issn
1432-8798journal_volume
151pub_type
杂志文章abstract：:A previously undescribed badnavirus was isolated from Zamia fischeri showing symptoms of chlorosis, necrosis, and ringspot. The virus has bacilliform virions 30 nm in diameter and averaging 120 nm in length. The viral genome is 9227 bp in length and contains three open reading frames characteristic of members of the g...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04630-6
更新日期：2020-07-01 00:00:00
abstract：:Noroviruses belong to a genus of genetically diverse viruses within the family Caliciviridae and cause acute gastroenteritis in humans and animals. They are subdivided into genogroups, each of which further segregates into genotypes. Until recently, a new genotype was based on a defined pairwise distance cutoff of com...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1708-5
更新日期：2013-10-01 00:00:00
abstract：:The diagnosis of cytomegalovirus retinitis (CMV-R) is difficult and usually based on clinical criteria or invasive diagnostic procedures. The purpose of this study was to investigate a possible association between CMV-R and specific anti-CMV antibodies in tears. Paired tear and serum samples were obtained from 96 indi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0813-0
更新日期：2006-12-01 00:00:00
abstract：:We previously reported the VP4 and the VP7 genotypes of the first G6P[14] rotavirus strain (RVA/Human-wt/GHA/M0084/2010/G6P[14]) from the stool of an infant with diarrhoea in Ghana. In the current study, we obtained the complete genome sequences using Illumina MiSeq next-generation sequencing to enable us to determine...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04543-4
更新日期：2020-04-01 00:00:00
abstract：:Although surfactant-secreting type II alveolar cells have been shown to be damaged during influenzavirus pneumonia, little is known about the effects of surfactant replacement therapy. We have developed a mouse influenza model, in which viral infection can be localized to the upper respiratory tract or to both the upp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050535
更新日期：1999-01-01 00:00:00
abstract：:The alkaloids contained in Colchicum autumnale seeds are used in numerous medicines. Good quality seeds are difficult to obtain from this undomesticated plant. Therefore, a research program was set up aiming to cultivate C. autumnale in order to improve alkaloid contents and seed yields. In this context, a collection ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0307-x
更新日期：2004-07-01 00:00:00
abstract：:A well characterized strain of guinea pig cytomegalovirus (GPCMV) was used to infect pregnant guinea pigs during various periods of pregnancy. Transplacental transmission of virus with invasion of the fetus was observed, even in some mothers with preinoculation evidence of GPCMV antibody. Fetal infection occurred duri...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317422
更新日期：1979-01-01 00:00:00
abstract：:Persistent infections with high-risk human papillomaviruses (HR-HPVs) are linked to the development of cervical cancer due to a deregulation of the productive viral cycle in the host cell, leading to cell transformation. The E2 viral protein is expressed early during an HPV infection and regulates viral replication an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-04124-6
更新日期：2019-07-01 00:00:00
abstract：:To evaluate the impact of new antiretroviral combinations (HAART: Highly Active Anti Retroviral Therapy) on HCV replication and liver enzyme levels, we analysed the changes in HCV viremia and aminotransferase levels in HIV and HCV co-infected patients. Moreover, to evaluate the influence of HCV infection on the effica...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050685
更新日期：2000-01-01 00:00:00
abstract：:Mycobacteriophage archival stocks have been kept for ca. 20-50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreov...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3788-8
更新日期：2018-07-01 00:00:00
abstract：:The complete nucleotide sequence of Subterranean clover mottle virus (SCMoV) genomic RNA has been determined. The SCMoV genome is 4,258 nucleotides in length. It shares most nucleotide and amino acid sequence identity with the genome of Lucerne transient streak virus (LTSV). SCMoV RNA encodes four overlapping open rea...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0144-3
更新日期：2003-11-01 00:00:00
abstract：:Human leukocyte antigen (HLA) alleles are associated with both the progression of chronic hepatitis C (CHC) and the sustained virological response (SVR) to antiviral therapy. HLA-A*02 is the most common HLA allele in people of European/Caucasian descent and the Chinese and Japanese population. Therefore, we investigat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2361-y
更新日期：2015-04-01 00:00:00
abstract：:We have compared the RNA synthesis of alfalfa mosaic virus in complete (by RNAs 1, 2 and 3) and incomplete infections (by RNAs 1 and 2) of cowpea protoplasts. Both viral RNA polymerase activity and accumulation of viral RNA were measured. By annealing RNA in solution with 32P-labelled probes of plus and minus polarity...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050305
更新日期：1998-01-01 00:00:00
abstract：:Using a one-step model for cell-to-cell transmission of HIV infection we have identified two distinct phases of HIV RNA synthesis. The first phase (4 h-12 h p.i.) was marked by an increase in only the full-length 9 kb RNA, while the second phase (24 h p.i. onwards) comprised a significant increase in the levels of all...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050429
更新日期：1998-01-01 00:00:00
abstract：:Murine flank scarification with HSV-1 and -2 results in primary lesions at the site of inoculation within three days and lesions at secondary sites within four days. The severity of the infection can be given a numerical value or "score" which is derived from the number and size of these lesions. Using this model, we ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050030
更新日期：2000-01-01 00:00:00
abstract：:The predominant field strains of Marek's disease virus in Guangxi were clearly different from the vaccine strain CVI988/Rispens based on sequencing of the envelope glycoprotein I (gI), glycoprotein E (gE) and oncogenic meq genes. These differences may be partly responsible for the most recent outbreaks in Guangxi. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0840-8
更新日期：2011-02-01 00:00:00
abstract：:Sera and bronchial washings from normal mice and from mice previously infected with influenza virus were analyzed for their concentration of four immunoglobulin (Ig) classes by the method of single radial immunodiffusion and for their content of specific antibody of these Ig classes by the immunofluorescent method. Ig...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314846
更新日期：1977-01-01 00:00:00
abstract：:This is the first sequence-based characterization of Mal de Río Cuarto virus (MRCV), currently classified as a variant of Maize rough dwarf virus (MRDV) and exclusively found in South America. We sequenced and analyzed genome segments S4 and S8. MRCV S4 coded for a putative 131.67 kDa protein while MRCV S8 coded for a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0840-4
更新日期：2002-09-01 00:00:00
abstract：:The nucleotide sequences of the 3' leader and 5' trailer regions were determined for genomic RNA of bovine respiratory syncytial virus (BRSV) strain A-51908. The leader and trailer sequences are '45' and '161' nucleotides in length, respectively. The functionality of BRSV leader and trailer sequences and their recogni...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050719
更新日期：1999-01-01 00:00:00
abstract：:Intravenous polyoma virus inoculation into pregnant Wistar rats resulted in transplacental infection of the foetus, causing tumours and hydronephroses. Cyclosporin A reduced these effects significantly. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313783
更新日期：1993-01-01 00:00:00
abstract：:There were three epidemic waves of human infection with avian influenza A (H7N9) virus in 2013-2014. While many analyses of the genomic origin, evolution, and molecular characteristics of the influenza A (H7N9) virus have been performed using sequences from the first epidemic wave, genomic characterization of the viru...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2872-1
更新日期：2016-08-01 00:00:00
abstract：:We determined the genome sequence of a new polerovirus that infects field pea and faba bean in China. Its entire nucleotide sequence (6021 nt) was most closely related (83.3% identity) to that of an Ethiopian isolate of chickpea chlorotic stunt virus (CpCSV-Eth). With the exception of the coat protein (encoded by ORF3...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1301-3
更新日期：2012-07-01 00:00:00
abstract：:Multiple splicing sites exist in the RNA genome of the human immunodeficiency virus type 1 (HIV-1). In a screen for subgenomic forms of the HIV-1 genome that could be transferred to fresh cells by virus infection, we identified a novel spliced variant of HIV-1 RNA that uses a hitherto unknown splice acceptor site with...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718159
更新日期：1996-01-01 00:00:00
abstract：:At low pH influenza virus causes membrane fusion. This phenomenon is thought to reflect a part of the infection mechanism of the virus. To obtain more information on the effect of low pH on the virus, the change in morphology of influenza virus particles was studied by electron microscopy. Further, the degradation of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311162
更新日期：1984-01-01 00:00:00
abstract：:The contribution of expression of human cytomegalovirus (HCMV) immediate early (IE) genes to the rapid and transient increase in cellular (c)-oncogene (fos, jun, myc) transcription following HCMV infection was investigated. A partial temporal overlap was observed between the increases in c-oncogene RNA levels and the ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314027
更新日期：1991-01-01 00:00:00
abstract：:In order to investigate the mechanism of long-term immunity and the effect of protective immunity induced by DNA vaccination, we constructed the expression plasmid containing a pseudorabies virus (PRV) gD gene encoding an envelope glycoprotein. Intramuscular vaccination of mice with the plasmid DNA induced a strong an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050272
更新日期：1998-01-01 00:00:00
abstract：:HSV-2 infections suppress the antibody response to HSV-1 but do not impair the mouse cytomegalo virus (MCMV) antibody generating system. In contrast, the sheep red blood cell (SRBC) IgM response is impaired by preinfections with HSV-2 but not with HSV-1. From the time kinetics of this suppression it can be concluded t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310741
更新日期：1987-01-01 00:00:00
abstract：:To provide information on the mechanism of perpetuation of influenza viruses among waterfowl reservoirs in nature, virological surveillance was carried out in Alaska during their breeding season in summer from 1991 to 1994. Influenza viruses were isolated mainly from fecal samples of dabbling ducks in their nesting pl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322743
更新日期：1995-01-01 00:00:00
abstract：:The precipitating and neutralizing activities of normal bovine sera with FMDV were studied and compared. Twenty-two out of 79 normal bovine sera gave a positive reaction in micro neutralization tests with FMDV type O, while six did so with type A. In RID tests 32 sera were positive with type O and 28 with type A virus...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315533
更新日期：1978-01-01 00:00:00
abstract：:Three distinct sequence groups were found among partial nucleotide sequences of 38 isolates of beet western yellows virus (BWYV) and beet mild yellowing virus (BMYV) from Europe, Iran and the USA. The first group contains both sugar beet and oilseed rape specific isolates, and the differentiating characteristic linked...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01323239
更新日期：1995-01-01 00:00:00
