Abstract:
:We constructed three sub-genomic replicons of Tick-borne encephalitis virus (TBEV) (Oshima REP, Oshima REP-GFP and Oshima REP-Neo) by deleting genes coding for structural proteins without or with insertion of green fluorescent protein (GFP) or Neo genes, respectively. BHK cells transfected with Oshima REP expressed the viral non-structural antigens in immunofluorescent and western blot analyses. GFP and viral antigens were co-expressed in the transfected cells with Oshima REP-GFP. G418-resistant cells harboring Oshima REP-Neo consistently expressed the antigens without showing any apparent CPE. These replicons constructed in this study will be useful in studies on the replication, assembly and packaging of TBEV, and to develop vaccines and gene-delivering systems.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Hayasaka D,Yoshii K,Ueki T,Iwasaki T,Takashima Idoi
10.1007/s00705-003-0262-ykeywords:
subject
Has Abstractpub_date
2004-06-01 00:00:00pages
1245-56issue
6eissn
0304-8608issn
1432-8798journal_volume
149pub_type
杂志文章abstract::Oral inoculation of entomopoxvirus spindles, microstructures composed of fusolin protein, causes disruption of the peritrophic matrix (PM), a physical barrier against microbe infection, in the insect midgut. Although the atomic structure of fusolin has been determined, little has been directly elucidated of the mechan...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-4013-5
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310767
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journal_title:Archives of virology
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doi:10.1007/s00705-014-2238-5
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0411-y
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0793-y
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1810-8
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journal_title:Archives of virology
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doi:10.1007/s00705-015-2413-3
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1973-3
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311220
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pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04702-7
更新日期:2020-09-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310741
更新日期:1987-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0184-8
更新日期:2004-02-01 00:00:00
abstract::The genomic sequence of an El Amar isolate of plum pox virus (PPV) from Egypt was determined by sequencing overlapping cDNA fragments. This is the first complete sequence of a member of the El Amar (EA) strain of PPV. The genome consists of 9791 nt, excluding a poly(A) tail at the 3' terminus. The complete nt sequence...
journal_title:Archives of virology
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journal_title:Archives of virology
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doi:10.1007/s00705-015-2504-1
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309845
更新日期:1993-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0307-3
更新日期:2009-01-01 00:00:00
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journal_title:Archives of virology
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170048
更新日期:2001-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050009
更新日期:2000-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01322681
更新日期:1995-01-01 00:00:00
abstract::Porcine astrovirus (PAstV) is widely distributed and highly prevalent among pigs, nevertheless its clinical significance remains unclear as it can be detected in both diarrheic and in healthy pigs. Information about the prevalence, clinical significance and molecular characterization of PAstV in Thailand is not availa...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3806-x
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pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050610
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314574
更新日期:1981-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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