Differential sensitivity of two related viruses, Newcastle disease virus and Sendai virus, to interferon in mouse Had-2 cells: selective inhibition of translation of NDV mRNA.

Abstract:

:Had-2, a mouse mutant cell line derived from FM3A, constitutively releases interferon-alpha and beta and acquires resistance to Newcastle disease virus (NDV) and other viruses. However, Had-2 was found as susceptible to Sendai virus (HVJ) as FM3A. Even when Had-2 cells were infected simultaneously with NDV and HVJ, only the replication of NDV was inhibited, while that of HVJ was not. Northern blot hybridization analysis indicated that accumulation of NDV-specific primary transcripts was somewhat reduced in Had-2, but the reduction was insufficient to critically suppress the viral replication. Moreover, this decrease was not observed in the presence of cycloheximide, and a closely comparable amount of the primary transcripts was detected in both Had-2 and FM3A cells. The mRNA accumulated in the presence of cycloheximide was translated efficiently on removal of the inhibitor in FM3A cells, but not at all in Had-2 cells. Thus the translation of NDV mRNA was the major target of interferon in Had-2 cells. The fact that the synthesis of HVJ proteins was unaffected in Had-2 cells may imply that a host-cell component that distinguishes between NDV and HVJ mRNAs is involved in their translation.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Aoki K,Kawakita M

doi

10.1007/BF01718199

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

1847-62

issue

10

eissn

0304-8608

issn

1432-8798

journal_volume

141

pub_type

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