Detection of Epstein-Barr virus infection in the epithelial cells and lymphocytes of non-neoplastic tonsils by in situ hybridization and in situ PCR.

Abstract:

:Non-neoplastic tonsils were analyzed for detection of Epstein-Barr virus (EBV)-positive cells by in situ hybridization and in situ PCR. EBV-encoded small nuclear RNA 1(EBER1)-positive cells were found in 28.2% of the tonsils and were evenly localized in the extrafollicular area and within germinal centers. Latent membrane protein 1 (LMP1)-positive cells were also dispersed in the extrafollicular and germinal center. Using in situ DNA-DNA hybridization, the EBV-positive signals were observed in the upper epithelial cell layers of the tonsils. In addition, in situ PCR detected EBV DNA-positive cells in the lower epithelial cell layers and lymphoid cells.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Kobayashi R,Takeuchi H,Sasaki M,Hasegawa M,Hirai K

doi

10.1007/s007050050332

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

803-13

issue

4

eissn

0304-8608

issn

1432-8798

journal_volume

143

pub_type

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