Abstract：
:Cell cultures inoculated with caprine respiratory syncytial virus (RSV) were studied with light, fluorescent, and electron microscopy to determine the morphogenesis and structure of the virus. Small syncytia were seen after 36 hours in culture. After 48 hours in culture, syncytia were large and numerous and pleomorphic cytoplasmic inclusions were seen. These inclusions were more pronounced and numerous later in the infection cycle. Indirect immunofluorescence revealed a diffuse to granular cytoplasmic fluorescence with fluorescing fibrils on the cell surface. With the electron microscope, filamentous (100-160 nm) and spherical (90-160 nm) particles were seen budding off the cell membrane. The number of virus buds diminished with increased size of syncytia. Granular pleomorphic cytoplasmic inclusions were seen near the nucleus, and electron dense masses were seen just beneath the cytoplasmic membrane where large quantities of virus were budding from the cell surface. The first type of inclusion had distinct borders; the second diffuse borders and appeared to contain viral nucleoprotein. Negative staining revealed spherical, pleomorphic, and filamentous forms of the virus; the last form predominated. The virions were covered with club-shaped projections, and the nucleocapsids were seen as fragile strands frequently broken into fragments or as isolated rings. Morphogenesis and structure of the caprine RSV places this virus with the Pneumovirus genus of the Paramyxoviridae family.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Lehmkuhl HD,Smith MH,Cutlip RCdoi
10.1007/BF01314543subject
Has Abstractpub_date
1980-01-01 00:00:00pages
269-76issue
3-4eissn
0304-8608issn
1432-8798journal_volume
65pub_type
杂志文章abstract：:Our previous studies demonstrated that hepatitis E virus (HEV) requires the multivesicular body (MVB) pathway to release virus particles, suggesting that HEV utilizes the cellular ESCRT machinery in the cytoplasm, not at the cell surface, to be released from infected cells. In this study, we generated a murine monoclo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1912-3
更新日期：2014-05-01 00:00:00
abstract：:Human bocavirus (HBoV) is a new parvovirus associated with acute respiratory tract infection (ARTI). In order to evaluate HBoV significance as an agent of acute respiratory disease, we screened 1,135 respiratory samples from children and adults with and without symptoms during two complete calendar years. HBoV1 preval...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2238-5
更新日期：2015-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3838-2
更新日期：2018-08-01 00:00:00
abstract：:Noroviruses belong to a genus of genetically diverse viruses within the family Caliciviridae and cause acute gastroenteritis in humans and animals. They are subdivided into genogroups, each of which further segregates into genotypes. Until recently, a new genotype was based on a defined pairwise distance cutoff of com...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1708-5
更新日期：2013-10-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3123-1
更新日期：2017-02-01 00:00:00
abstract：:Hepatitis E represents an important public-health concern throughout the world. It is one of the leading causes of hepatitis in North Africa, Asia and the Middle East. In Tunisia, the true burden of HEV infection is still unknown. The objectives of the present study were to assess the occurrence of hepatitis E virus i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2251-8
更新日期：2015-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314980
更新日期：1980-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3715-z
更新日期：2018-05-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0491-3
更新日期：2005-06-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316736
更新日期：1986-01-01 00:00:00
abstract：:Porcine astrovirus (PAstV) is widely distributed and highly prevalent among pigs, nevertheless its clinical significance remains unclear as it can be detected in both diarrheic and in healthy pigs. Information about the prevalence, clinical significance and molecular characterization of PAstV in Thailand is not availa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3806-x
更新日期：2018-07-01 00:00:00
abstract：:PCR products representing c. 550 3' terminal bases of Beet soil-borne pomovirus (BSBV) RNA 3 were compared for sources of this virus from all major sugarbeet-growing areas in Germany. In none of these areas conspicious symptoms could be attributed to the presence of BSBV. Single strand conformation polymorphism analys...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070117
更新日期：2000-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1833-1
更新日期：2014-02-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2125-0
更新日期：2014-10-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3741-x
更新日期：2018-05-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2649-y
更新日期：2016-02-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050054
更新日期：2000-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1731-6
更新日期：2014-03-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1396-6
更新日期：2012-11-01 00:00:00
abstract：:Restriction maps of cervid herpesviruses 1 and 2 which are antigenetically related to bovine herpesvirus 1, were deduced from Southern blot hybridization with HindIII restriction fragments of BHV-1 DNA as probes. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309448
更新日期：1993-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00705-020-04594-7
更新日期：2020-07-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0231-5
更新日期：2004-03-01 00:00:00
abstract：:Infectious cDNA clones of the Shanghai isolate of Ribgrass mosaic virus (RMV) were produced by joining four overlapping cDNA fragments and also in a single step by long template PCR. After inoculation of Nicotiana glutinosa with either RNA transcripts or the cDNA under the control of the CaMV 35S promoter, plants deve...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0740-0
更新日期：2006-09-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718833
更新日期：1996-01-01 00:00:00
abstract：:The complete sequence of 8159 nucleotides of the double stranded DNA genome of cassava vein mosaic virus (CsVMV) was determined (# U59751) and revealed a significant difference in genome organization when compared with a previous report (# U20341). When transferred to cassava plants by microbombardment, the full lengt...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050344
更新日期：1998-01-01 00:00:00
abstract：:Viral infections of the central nervous system (CNS) following peripheral inoculation of Sindbis viruses were studied. The use of viral strains, which vary in their neuroinvasive and neurovirulent properties, and various strains of mice, which differ in immunocompetence, revealed several pathways of viral neuroinvasio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050576
更新日期：1999-01-01 00:00:00
abstract：:An attempt has been made to devise computer software that will aid virologists to identify unknown virus isolates using the World Wide Web. Computerized information from the Animal Virus Information System was used to obtain data on various characters of a virus species. Sequence data banks are used to obtain the mole...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050394
更新日期：1998-01-01 00:00:00
abstract：:The nucleotide sequence of the 3' terminal region of the Australian isolate of papaya ringspot virus type W [PRSV-W (Aust)] was determined. An open reading frame (864 bp), encoding the putative coat protein gene, occurs upstream of the putative 3' untranslated region (206 bp) and poly(A) tail. A 23 amino acid sequence...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317141
更新日期：1992-01-01 00:00:00
abstract：:The nucleotide sequence of genomic RNA of Marburg virus strain Popp was determined. Strain Popp was isolated in 1967 during the first filoviral outbreak. The virus was purified from blood of infected guinea pigs in which it had been maintained. The length of the determined sequence was 19112 nucleotides. Amino acid se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322532
更新日期：1995-01-01 00:00:00
abstract：:The high prevalence (14.3%) of HIV/HBV co-infections reported in west China makes it necessary to screen concurrent HBV infection in HIV carriers. HBV B genotype was shown to be dominant in 54 cases of HIV/HBV co-infection, accounting for 81.48% of the total. The total drug resistance rate observed was 3.70%. A1762T, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3900-0
更新日期：2018-10-01 00:00:00
