Abstract：
:Hepatitis E represents an important public-health concern throughout the world. It is one of the leading causes of hepatitis in North Africa, Asia and the Middle East. In Tunisia, the true burden of HEV infection is still unknown. The objectives of the present study were to assess the occurrence of hepatitis E virus in Tunisia through the monitoring of urban sewage and to characterize the strains identified using molecular assays. A total of 150 sewage samples (raw and treated) were collected from three wastewater treatment plants located in the regions of Monastir and Mahdia and analyzed by nested RT-PCR using a qualitative assay targeting the methyltransferase gene in ORF1. Of these, only three samples (2 %) were found to be positive for HEV, one belonging to genotype 1 and two to genotype 3. The results of the present study indicate a low level of virus excretion among the Tunisian population. Both genotypes 1 and 3 are circulating in this country, however, possibly causing sporadic infections. The presence of the zoonotic genotype 3, known to be transmitted to humans mainly by swine and demonstrated in Tunisia for the first time in this work, raises the question of possible reservoir species, since pork products are not consumed in this country, pigs are not bred, and wild boar is not endemic. Further studies will be needed to gather information on the occurrence and diversity of HEV strains circulating among humans and animals in Tunisia, and on possible animal reservoirs.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Béji-Hamza A,Hassine-Zaafrane M,Khélifi-Gharbi H,Della Libera S,Iaconelli M,Muscillo M,Petricca S,Ciccaglione AR,Bruni R,Taffon S,Aouni M,La Rosa Gdoi
10.1007/s00705-014-2251-8subject
Has Abstractpub_date
2015-01-01 00:00:00pages
183-9issue
1eissn
0304-8608issn
1432-8798journal_volume
160pub_type
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