Sequence analysis of a parvovirus B19 isolate and baculovirus expression of the non-structural protein.

Abstract:

:Serology for parvovirus B19 has been hampered by limited availability of antigen which has often had to be isolated from viraemic blood donations. We have determined the sequence of the genome of one such isolate (Stu). It is 99% similar to the sequences of two other isolates (Wi and Au) except at the far 5'-end, where it is more similar to the terminus of another isolate (Ala/Alb). Recombinant nonstructural protein, NS, was constructed. Antibodies to NS, as well as to the capsid proteins, VP1/2, were detected in patients with B19 infection.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Hicks KE,Cubel RC,Cohen BJ,Clewley JP

doi

10.1007/BF01718833

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

1319-27

issue

7

eissn

0304-8608

issn

1432-8798

journal_volume

141

pub_type

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