Abstract:
:A Luminex xTAG-based assay for plant-infecting tospoviruses was developed. The test enables the detection of tospoviruses in general and the differentiation of the four important member species of this genus: Tomato spotted wilt virus, Impatiens necrotic spot virus, the proposed 'Capsicum chlorosis virus' and Watermelon silver mottle virus. The generic tospovirus primers used in this method are also applicable for detection of tospoviruses by basic RT-PCR. We also describe an economic alternative method for the distinction of the four tospoviruses mentioned and of additional member viruses, based on a restriction fragment length polymorphism (RFLP). The sophisticated Luminex xTAG technology allows the simultaneous detection of various targets. This study is part of a project that aims to develop a method for the simultaneous detection of various plant pathogens (viral, bacterial and fungal) in plant material.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Bald-Blume N,Bergervoet JHW,Maiss Edoi
10.1007/s00705-017-3256-xsubject
Has Abstractpub_date
2017-06-01 00:00:00pages
1519-1528issue
6eissn
0304-8608issn
1432-8798pii
10.1007/s00705-017-3256-xjournal_volume
162pub_type
杂志文章abstract::orf124 (ac124) of AcMNPV is one of the highly conserved unique genes in group I lepidopteran nucleopolyhedroviruses. So far, its function remains unknown. In this study, infection with a virus expressing an ac124-gfp fusion showed that Ac124 localized to the cytoplasm throughout the infection. In addition, an ac124 kn...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2277-y
更新日期:2015-01-01 00:00:00
abstract::Two cases of coinfection with bovine papular stomatitis virus (BPSV) and pseudocowpox virus (PCPV) in dairy calves in Tochigi Prefecture, Japan, are reported. Sequences of BPSV and PCPV were simultaneously detected in the same polymerase chain reaction (PCR) amplicons, which were obtained from the DNA of two dairy cal...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04792-3
更新日期:2020-11-01 00:00:00
abstract::The clinical performance of the newly developed MeltPro® HPV Test, based on multicolor melting curve analysis, was evaluated and compared with the commercially available Cobas® HPV Test for detection of HPV and genotyping of HPV-16 and HPV-18. A total of 1647 cervical samples were analyzed with both tests. The agreeme...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3645-1
更新日期:2018-03-01 00:00:00
abstract::Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3260-1
更新日期:2017-07-01 00:00:00
abstract::The infectivity of the H9N2 virus to MDCK cells was time-dependently inhibited by Cu(2+) at concentrations of 2.5-250 microM. In 25 microM Cu(2+) solution, the virus titer decreased by approximately 3 and 4 log within 3 and 6 h, respectively. Compared to Cu(2+), Zn(2+) was much less effective in virus inactivation. Th...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0154-2
更新日期:2008-01-01 00:00:00
abstract::By reverse transcription (RT)-nested polymerase chain reaction (PCR), hepatitis E virus (HEV) RNA was simultaneously detected in the livers of aborted fetuses and in fecal and serum samples from their sows, obtained from two of 14 farms tested. Sequence and phylogenetic analyses showed that these HEVs belonged to geno...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0679-z
更新日期:2010-07-01 00:00:00
abstract::Dengue and chikungunya viruses co-circulate and cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously dete...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2217-x
更新日期:2015-01-01 00:00:00
abstract::The stools of slaughtered pigs were screened for hepatitis E virus (HEV). HEV RNA was detected in 7.3% of the samples. HEV strains were characterized as genotype 3 subtype c, a cluster previously not described in Italy. These findings provide evidence that slaughterhouse workers may be exposed to HEV infection. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0544-0
更新日期:2010-01-01 00:00:00
abstract::An apparently single rotavirus A strain possessing a genotype constellation of G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 abruptly emerged, caused diarrhoea in children requiring hospitalisation, and increased to reach 27 % of strains detected during the first half of 2015 in Vietnam. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2682-x
更新日期:2016-02-01 00:00:00
abstract::Poultry production is an important sector of agriculture in Sri Lanka; however, there is a lack of information regarding circulation of infectious bronchitis virus (IBV). RNA was extracted from chicken tissues, subjected to IBV S1 RT-PCR, and sequenced. Overall, 19 out of 34 (55.88 %) samples were IBV positive and con...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2831-x
更新日期:2016-06-01 00:00:00
abstract::Internal papillomatosis of parrots (IPP) is a tumour disease with unknown etiology, characterised by progressive development of papillomas in the oral and cloacal mucosa. Based on epidemiologic data, infectious agents, particularly DNA tumour viruses, are considered to be involved. In this study, cloacal papillomas we...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0858-7
更新日期:2002-10-01 00:00:00
abstract::We investigated the frequency and the clinical relevance of hepatitis B virus (HBV) pre-S mutations in Qidong, China. The results showed HBV pre-S mutants were detected in 48.4% (47/97) of patients with HBV infection. Both pre-S deletion and pre-S2 start codon mutations were more frequently found in HCC than in CH pat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0176-9
更新日期:2008-01-01 00:00:00
abstract::Alfamo- and ilarviruses are characterized by the deficiency of their genomes (three messenger-sense RNAs) to start an infection cycle. The RNAs are in capsids built from a single species of protein of about 24 kD. A few dimers of this coat protein per RNA molecule are sufficient to activate the genome. Since the first...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s007050050551
更新日期:1999-01-01 00:00:00
abstract::Anti-human T-lymphotropic virus type I/II (HTLV-I/II) antibodies were screened by particle agglutination test in a total of 66 patients with thalassemia major who received multiple transfusion from paid donors at the Blood Transfusion Hematology Center of Ho Chi Minh City in South Vietnam. HTLV-II infection was confir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050170
更新日期:1997-07-01 00:00:00
abstract::During infection host histo-blood group antigens (HBGAs) act as attachment factors that interact with rabbit hemorrhagic disease virus (RHDV) and participate in the infectious process. In the present study, baculovirus expressing recombinant RHDV capsid protein (VP60r) as a vaccine immunogen was used to test its antig...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3509-8
更新日期:2017-11-01 00:00:00
abstract::Hepatitis B virus (HBV) is one of the most prevalent and infectious agents that leads to liver disease in humans. Five clinical forms of HBV infection exist, including fulminant, acute, chronic, asymptomatic and occult. The chronic, asymptomatic and occult forms are long-term infections that can lead to hepatocellular...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s00705-014-2002-x
更新日期:2014-08-01 00:00:00
abstract::The complete sequence of 8159 nucleotides of the double stranded DNA genome of cassava vein mosaic virus (CsVMV) was determined (# U59751) and revealed a significant difference in genome organization when compared with a previous report (# U20341). When transferred to cassava plants by microbombardment, the full lengt...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050344
更新日期:1998-01-01 00:00:00
abstract::A total of 289 cloacal swabs from pigeons from 29 different breeders in Germany were collected. In addition, samples from pigeons exhibited at shows were collected. The detailed health status of the pigeon flocks was recorded. Samples were analysed for the presence of the recently discovered pigeon rotavirus and pigeo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04834-w
更新日期:2021-01-01 00:00:00
abstract::An F35L mutation in the N-terminal domain of the polymerase acidic protein (PA-Nter), which contains the active site of the endonuclease, has been reported to result in higher polymerase activity in mouse-adapted strains of the 2009 pandemic influenza A H1N1 virus. We modeled wild and mutant complexes of uridine 5'-mo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3681-x
更新日期:2018-04-01 00:00:00
abstract::Infectious pancreatic necrosis virus exhibited an interference phenomenon that resulted in the survival of the infected cell with one hit kinetics. The responsible factor was found to co-purify with standard virus through a purification regime that employed two CsCl gradients and a sucrose gradient. This result sugges...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315639
更新日期:1978-01-01 00:00:00
abstract::The genome of a Spanish isolate of Broad bean wilt virus-1 (BBWV-1) was completely sequenced and compared with available sequences of other isolates of the genus Fabavirus (BBWV-1 and BBWV-2). This consisted of two RNAs of 5814 and 3431 nucleotides, respectively, and their organization was similar to that of other mem...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-005-0582-1
更新日期:2005-10-01 00:00:00
abstract::This study aimed at investigating the effect of progesterone on interferon signaling pathways in peripheral blood mononuclear cells (PBMCs) of patients infected with hepatitis C virus (HCV). PBMCs were isolated from peripheral blood of 38 treatment-naïve HCV-infected patients, pooled, and stimulated with progesterone ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1673-z
更新日期:2013-08-01 00:00:00
abstract::A high per cent of false positive results for rubella virus IgM antibodies determined by immune fluorescence were detected in sera of pregnant women. After absorption with Staphylococcus suspension, Cowan 1 strain, and aggregates of human immunoglobulins, the false positive reactions due to IgM antiimmunoglobulin were...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01348028
更新日期:1979-01-01 00:00:00
abstract::Wild plants of the family Convolvulaceae are hosts for a few New World begomoviruses (genus Begomovirus, family Geminiviridae). In this work, we report the complete genome sequence of a new begomovirus infecting the wild convolvulaceous plant Jacquemontia tamnifolia in Venezuela. The cloned bipartite genome showed the...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3372-7
更新日期:2017-08-01 00:00:00
abstract::In the Mt. Kosciusko alpine area of Australia there are three well-separated populations of Cardamine lilacina, an endemic sward-forming perennial brassica, and these are infected with turnip yellow mosaic tymovirus. The genetic variation in these viral populations has been assessed by an RNA hybrid mismatch polymorph...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309845
更新日期:1993-01-01 00:00:00
abstract::The complete nucleotide sequence of Pelargonium flower break virus (PFBV) has been determined. The genomic RNA is 3923 nucleotides (nt) long and contains five open reading frames (ORFs). The 5'-proximal ORF encodes a 27 kDa protein (p27) and terminates with an amber codon which may be read-through into an in-frame p56...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0231-5
更新日期:2004-03-01 00:00:00
abstract::Genetic relationships among echovirus type 25 (ECV 25) isolates associated with aseptic meningitis in Germany 1997/98 and a 40-year-old ECV 25 prototype strain were investigated using RT-PCR and direct sequencing the VP1/2A gene junction region. Sequences were compared to each other and to non-polio enterovirus repres...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050640
更新日期:1999-01-01 00:00:00
abstract::Cowpea and broad bean plants showing severe stunting and leaf rolling symptoms were observed in Hefei city, Anhui province, China, in 2014. Symptomatic plants from both species were shown to be infected with milk vetch dwarf virus (MDV) by PCR. The complete genomes of MDV isolates from cowpea and broad bean were seque...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3348-7
更新日期:2017-08-01 00:00:00
abstract::Extensive heterogeneity in electropherotypes observed among group A human rotaviruses has been considered as a result of two major mechanisms; i.e., the accumulation of point mutations and genetic reassortment between concurrently-circulating strains. However, no evidence was reported thus far indicating that any one ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170162
更新日期:2001-01-01 00:00:00
abstract::Cumulative studies have demonstrated that dengue virus infection results in the induction of apoptosis of certain cells in vitro. Moreover, apoptosis of microvascular endothelial cells in the brain and in the intestinal serosa has been demonstrated postmortem in dengue virus (DENV)-infected patients. In this work, hum...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0396-7
更新日期:2009-01-01 00:00:00