Abstract:
:The exact intracellular site of hepatitis A virus (HAV) production is unknown, possibly due to its usually slow and inefficient replication. Using immunocytochemistry and in-situ RT-PCR, we show that in cells infected with the rapidly replicating HAV strain HAS-15, viral proteins and RNA are scattered throughout the cytoplasm and accumulate in the perinuclear cytoplasmic area. Various ultrastructural alterations were found in infected cells, such as large polyribosomes, swelling of the perinuclear space and the ER, and dilatation of Golgi cisternae. In addition, HAV infection induced the formation of large arrays of annulate lamellae. Direct visualization of HAV particles was scarce. The various ultrastructural alterations described here might represent different phases of the replicative cycle of HAV that is asynchronous in the infected cell layer.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Klinger MH,Kämmerer R,Hornei B,Gauss-Müller Vdoi
10.1007/s007050170003keywords:
subject
Has Abstractpub_date
2001-12-01 00:00:00pages
2291-307issue
12eissn
0304-8608issn
1432-8798journal_volume
146pub_type
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journal_title:Archives of virology
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