Molecular detection of genotype 3 porcine hepatitis E virus in aborted fetuses and their sows.

Abstract:

:By reverse transcription (RT)-nested polymerase chain reaction (PCR), hepatitis E virus (HEV) RNA was simultaneously detected in the livers of aborted fetuses and in fecal and serum samples from their sows, obtained from two of 14 farms tested. Sequence and phylogenetic analyses showed that these HEVs belonged to genotype 3 HEV. HEV RNA was detected by real-time RT-PCR at 2.0 x 10(3)-2.8 x 10(4) viral copies/microl in the HEV-positive samples. All HEV-positive fetuses tested positive for porcine circovirus type 2 (PCV2). These results indicate that transplacental infection of HEV with PCV2 coinfection may occur in sows with reproductive failure, which is suggestive of similarities to HEV infection in women.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Hosmillo M,Jeong YJ,Kim HJ,Park JG,Nayak MK,Alfajaro MM,Collantes TM,Park SJ,Ikuta K,Yunoki M,Kang MI,Park SI,Cho KO

doi

10.1007/s00705-010-0679-z

subject

Has Abstract

pub_date

2010-07-01 00:00:00

pages

1157-61

issue

7

eissn

0304-8608

issn

1432-8798

journal_volume

155

pub_type

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