Abstract:
:In order to improve the knowledge of the humoral immune response to CMV infection, we developed an immunoblotting technique which allowed a better analysis of the changes in the pattern of anti CMV-polypeptides IgM. We examined 234 sera belonging to 27 renal allograft recipients developing a primary or recurrent CMV infection and 12 non infected recipients. Thus we found that 11 main anti CMV-polypeptides IgM antibodies were present in over 25% of the infected patients. They reacted with proteins whose molecular weights ranged from 32K to 205K. We showed that anti-p 45-47 IgM antibodies were present in 100% of CMV infected recipients and never in the non-infected population. They appeared very early in the course of the infection (5.43 weeks post-graft for primary infection and 5.00 weeks for recurrent ones) and, therefore, constitute a good marker of active infection. Two other CMV-specific IgM antibodies (anti-p 60-64 and anti-p 100) were found exclusively in the course of primary infections. Anti-p 60-64 IgM was observed at a high frequency (57.1%) and with a mean delay of 6.57 weeks post-graft. Therefore, the anti-p 60-64 IgM detection could be helpful for the diagnosis of primary infection. In almost 100% of both primary and recurrent infections, we observed anti-p 140 and anti-p 38 IgM antibodies. Only about 50% of non-infected patients had low levels of anti-p 140 and anti-p 38 IgM. The follow-up of recurrent infections showed that the anti CMV-polypeptides IgM antibodies appeared earlier than in primary infection. When we compared anti-p 45-47 IgM detection by immunoblotting and anti-CMV IgM detected by ELISA we observed that immunoblotting permitted the diagnosis 2.5 weeks earlier for primary infection, and 1 week earlier for recurrent infection, than ELISA. In addition, the detection of anti-p 45-47 IgM antibodies also occurred earlier than virus excretion.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Basson J,Tardy JC,Aymard Mdoi
10.1007/BF01310938subject
Has Abstractpub_date
1989-01-01 00:00:00pages
259-70issue
3-4eissn
0304-8608issn
1432-8798journal_volume
108pub_type
杂志文章abstract::Codiaeum variegatum (common name, garden croton) is an ornamental plant grown for its bright yellow variegated leaf morphology. Two C. variegatum plants with upward leaf curling and vein swelling symptoms were collected in Faisalabad, Pakistan. Sequencing of clones obtained by PCR amplification with specific primers s...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04633-3
更新日期:2020-08-01 00:00:00
abstract::Members of the family Closteroviridae have emerged as a major problem in agricultural crops in the past two decades. Diodia vein chlorosis virus (DVCV) is an understudied whitefly-transmitted closterovirus. Given the presence of the primary host for the virus in major agricultural production areas in the United States...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1055-3
更新日期:2011-11-01 00:00:00
abstract::R 61,837, a new antirhinovirus compound, was able to protect several susceptible rhinoviruses against inactivation by mild acidification or heat. This observation strengthens the hypothesis that the drug exerts antiviral activity by a direct interaction with the viral protein capsid to stabilize the particle. However,...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311037
更新日期:1989-01-01 00:00:00
abstract::An immunofluorescence test on smears of nasal epithelial cells was used to detect coronavirus infection in the respiratory tract of calves. Thirteen gnotobiotic calves were infected with coronavirus isolates derived from faeces or respiratory material: virus was detected in faeces and nasal swabs from all animals. In ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317007
更新日期:1985-01-01 00:00:00
abstract::Acute diarrhea outbreaks caused by porcine epidemic diarrhea virus (PEDV) have been observed in various pig-breeding provinces of China since December 2010. Endemic strains of PEDV were isolated from different areas, and the complete genome sequences of 10 isolates were determined. Our objective in this study was to g...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1767-7
更新日期:2013-12-01 00:00:00
abstract::The temperature sensitivity of herpes simplex virus type 1 (HSV-1) was assessed in primary cultures of mouse central nervous system (MNS) cells and mouse embryo cells (MEC). Infectious yields were determined and the ultrastructural morphogenesis of HSV-1 particles was compared following incubation at 37 or 40.5 degree...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309574
更新日期:1992-01-01 00:00:00
abstract::Cross hemagglutination inhibition (HI) and neuraminidase inhibition (NI) tests demonstrated strong antigenic relationship between A/Swine/Wisconsin/1/73 (SW/73) and A/Swine/Shope/15/31 (SW/31) influenza viruses. An eigthyone fold purification of virus was achieved by adsorption and elution followed by differential ult...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317967
更新日期:1975-01-01 00:00:00
abstract::Hepatitis E represents an important public-health concern throughout the world. It is one of the leading causes of hepatitis in North Africa, Asia and the Middle East. In Tunisia, the true burden of HEV infection is still unknown. The objectives of the present study were to assess the occurrence of hepatitis E virus i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2251-8
更新日期:2015-01-01 00:00:00
abstract::We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5' end of both RNA1...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1625-7
更新日期:2013-07-01 00:00:00
abstract::We present here the results of the analysis of the complete genome sequence of a lytic bacteriophage, vB_ButM_GuL6, which is the first virus isolated from Buttiauxella. Electron microscopy revealed that vB_ButM_GuL6 belongs to the family Myoviridae, order Caudovirales. The genome of vB_ButM_GuL6 is a linear, circularl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04780-7
更新日期:2020-11-01 00:00:00
abstract::Five cDNA probes prepared from molecular clones representing genomic RNA sequences of the virulent Miller strain of transmissible gastroenteritis virus (TGEV) were used in a dot blot hybridization assay to detect TGEV in cell culture and fecal specimens. Two clones (pA2 and pB4) represent nucleotide base pairs at the ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01319234
更新日期:1991-01-01 00:00:00
abstract::Non-nucleoside reverse transcriptase inhibitors (NNRTIs) are selective for human immunodeficiency virus type 1 (HIV-1) and generally not effective on HIV-2 or simian immunodeficiency virus (SIV). Only SIVagm was found to be sensitive to NNRTIs. When the amino acid differences in RT between SIVmac and SIVagm were compa...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170143
更新日期:2001-01-01 00:00:00
abstract::The alteration of whole Sendai virus and especially of its nucleocapsid polypeptides, during storage of the virus at 4 degree C in the allantoic fluids in which it was cultivated, has cultivated, has been studied by sodium dodecyl sulfate gel electrophoresis. During virus storage the nucleocapsid protein subunits with...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317427
更新日期:1975-01-01 00:00:00
abstract::The characterization of the aetiological agent of viral erythrocytic infection (VEI) of sea bass suggests a retroviral origin of the disease. RNA from viral erythrocytic infection virus (VEIV) and DNA from blood and organs of VEI-affected fish hybridized to a specific retrovirus cDNA probe. Sequences homologous to ret...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309960
更新日期:1995-01-01 00:00:00
abstract::Optimal conditions of radial haemolysis in gel (HIG) with Chikungunya (Chik) and Japanese encephalitis (JE) viruses were developed and compared with those of rubella virus. Sheep erythrocytes were found preferable for HIG with Chik and JE viruses, unlike rubella virus, where goose erythrocytes gave best results. Roost...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01320619
更新日期:1980-01-01 00:00:00
abstract::Sixteen patients with symptoms typical for Ockelbo disease (rash, arthralgia, fever) were enrolled in a 2 1/2 year study, during which clinical symptoms were recorded and ELISA was employed to study specific IgM, IgG and IgG subclass development. Initially, all patients presented with rash and arthralgia, and five pat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01379107
更新日期:1994-01-01 00:00:00
abstract::Modern adjuvants should induce strong and balanced immune responses, and it is often desirable to induce specific types of immunity. As an example, efficient Th1-immunity-inducing adjuvants are highly in demand. Such adjuvants promote good cell-mediated immunity against subunit vaccines that have low immunogenicity th...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s00705-010-0863-1
更新日期:2011-02-01 00:00:00
abstract::Reassortant H1 swine influenza viruses (SIVs) carrying 2009 pandemic H1N1 virus (pH1N1) genes have been isolated from pigs worldwide. Seven novel reassortant H3N2 SIVs were identified from diseased pigs in the USA from winter 2010 to spring 2011. These novel viruses contain three or five internal genes from pH1N1 and ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1203-9
更新日期:2012-03-01 00:00:00
abstract::The nucleotide sequence of the coat protein cistron of an Austrian isolate of grapevine fanleaf virus (GFLV-FC) from Vitis vinifera cv. French Colombard was determined. It shows small differences at the RNA level as well as at the protein level compared to the sequences of already published grapevine fanleaf virus str...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309730
更新日期:1995-01-01 00:00:00
abstract::Human cytomegalovirus (HCMV) has evolved several immune-evasion strategies. One strategy involves encoding Fcgamma receptor (FcγR)-like proteins that thwart the Fcγ-mediated effector functions. Our aim was to determine whether GM allotypes-immunoglobulin γ chain determinants expressed primarily on the Fc segment-modul...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-0937-8
更新日期:2011-05-01 00:00:00
abstract::To provide information on the mechanism of perpetuation of influenza viruses among waterfowl reservoirs in nature, virological surveillance was carried out in Alaska during their breeding season in summer from 1991 to 1994. Influenza viruses were isolated mainly from fecal samples of dabbling ducks in their nesting pl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01322743
更新日期:1995-01-01 00:00:00
abstract::As the most common NK/T-cell lymphoma in Asian countries, extranodal NK/T-cell lymphoma, nasal type (ENKTL), has unique clinical features and a strong association with Epstein-Barr virus (EBV). In order to gain a preliminary understanding of the relationship between ENKTL and EBV, we performed genotypic analysis of EB...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2451-x
更新日期:2015-08-01 00:00:00
abstract::Despite reports that the PB1-F2 protein contributes to influenza virus pathogenicity in the mouse model, little is known about its significance in avian hosts. In our previous study, the A/Vietnam/1203/04 (H5N1) wild-type virus (wtVN1203) was more lethal to mallard ducks than a reverse genetics (rg)-derived VN1203. In...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0666-4
更新日期:2010-06-01 00:00:00
abstract::Mosquito-borne alphaviruses are widely distributed throughout the world, causing important human illnesses. Therefore, the development of methods to enable early diagnosis of infections by alphavirus is essential. We show here the development and evaluation of a quantitative real-time RT-PCR using genus-specific prime...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3019-0
更新日期:2016-11-01 00:00:00
abstract::A real-time PCR assay based on primer-probe energy transfer (PriProET) was developed to detect swine vesicular disease virus (SVDV). Specificity tests of SVDV and heterologous virus showed specific amplification of SVDV strains only. The amplification plot for the closely related Coxsackievirus B5 remained negative. T...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0817-9
更新日期:2006-12-01 00:00:00
abstract::The complete sequence of a narcissus virus isolated from the Netherlands (Narv-NL) was determined to be 8172 nucleotides in length with an open reading frame encoding for 2624 amino acids. Phylogenetic analysis indicates that Narv-NL is clustered with high confidence among representative members from the genus Maclura...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3741-x
更新日期:2018-05-01 00:00:00
abstract::The global emergence of canine parvovirus type 2c (CPV-2c) has been well documented. In the present study, 139 rectal swab samples collected from diarrheic dogs living in Vientiane, Laos, in 2016 were tested for the presence of the canine parvovirus (CPV) VP2 gene by PCR. The results showed that 82.73% (115/139) of do...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3212-1
更新日期:2017-05-01 00:00:00
abstract::We previously reported the isolation of South River virus (SORV) from a pool of mosquitoes collected in the Yucatan Peninsula of Mexico (Farfan-Ale et al. in Vector Borne Zoonotic Dis 10:777-783, 5). The isolate (designated SORV-252) was identified as SORV after a 197-nucleotide region of its small RNA genome segment ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1280-4
更新日期:2012-06-01 00:00:00
abstract::Strains of an influenza H10N4 virus have been isolated during an outbreak of a respiratory disease in mink on the south-east coast of Sweden. This was the first example of a disease in mammals caused by the H10 subtype. We compared the A/mink/Sweden/84 strain with two recent avian H10N4 isolates, one from fowl and ano...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318353
更新日期:1990-01-01 00:00:00
abstract::Lolium latent virus (LoLV) was recently detected in the USA for the first time in ryegrass hybrids (Lolium perenne x Lolium multiflorum). The genome of one USA isolate, LoLV-US1, has now been fully sequenced. The positive strand genomic RNA is 7674 nucleotides (nt) long and is organized in five open reading frames (OR...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0108-8
更新日期:2008-01-01 00:00:00