Molecular characterization of canine parvovirus in Vientiane, Laos.

Abstract:

:The global emergence of canine parvovirus type 2c (CPV-2c) has been well documented. In the present study, 139 rectal swab samples collected from diarrheic dogs living in Vientiane, Laos, in 2016 were tested for the presence of the canine parvovirus (CPV) VP2 gene by PCR. The results showed that 82.73% (115/139) of dogs were CPV positive by PCR. The partial VP2 gene was sequenced in 94 of the positive samples; 91 samples belonged to CPV-2c (426Glu) subtype, while 3 samples belonged to the CPV-2a (426Asn) subtype. Notably, phylogenetic analysis of amino acid sequences revealed a close relationship between Laotian isolates and novel Chinese CPV-2c isolates. In Laotian CPV isolates, aligned protein sequences indicated a high rate of residue substitutions at positions 305, 324, 345, 370, 375, and 426 in the GH loop. The mutation at residue 370 (Q370R), a single mutation, was characterized as a unique mutant residue specific to the Laotian CPV-2c variant.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Vannamahaxay S,Vongkhamchanh S,Intanon M,Tangtrongsup S,Tiwananthagorn S,Pringproa K,Chuammitri P

doi

10.1007/s00705-016-3212-1

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

1355-1361

issue

5

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-016-3212-1

journal_volume

162

pub_type

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