Regulation of the Borna disease virus polymerase complex by the viral nucleoprotein p38 isoform. Brief Report.

Abstract:

:Borna disease virus (BDV) polymerase can be reconstituted by co-expression of the viral genes N, P and L. N codes for two proteins, p39 and p38, resulting from alternative translation initiation. Using a viral minireplicon system we observed that unlike p39, p38 was almost completely inactive when expressed with P and L alone. Since BDV polymerase requires a 10-20 fold excess of N protein over P for high activity, we determined whether p38 might serve as buffer that influences the viral N:P ratio. We demonstrate that p38 efficiently rescued BDV polymerase activity in cells expressing unfavorably high amounts of P.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Schneider U,Naegele M,Staeheli P

doi

10.1007/s00705-004-0327-6

keywords:

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

1409-14

issue

7

eissn

0304-8608

issn

1432-8798

journal_volume

149

pub_type

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