Abstract:
:Complete nucleotide sequences of cytoplasmic inclusion (CI) genes of two strains of potato virus Y (PVY) were determined from six polymerase chain reaction (PCR)-amplified cDNA clones. The size of the CI genes of both ordinary (PVY-O) and necrotic strains (PVY-T13) was 1902 nucleotides, with a sequence homology of 83.4%. Comparison of the predicted amino acid sequences showed more than 90% homology. When these were compared with those of other potyviruses, the homology ranged from 53 to 61%. cDNAs of all or a part of the PVY-O CI gene containing an additional initiation codon (ATG) at the 5' end and a stop codon at the 3' end were constructed by PCR amplification and cloned into an Escherichia coli expression vector, pKK 223-3. Complete and truncated PVY-O CI proteins were successfully produced in E. coli as judged by reactivities with PVY-O CI protein-specific antiserum. To our knowledge, this is the first report on expression of PVY CI proteins in E. coli.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Ohshima K,Inoue AK,Shikata Edoi
10.1007/BF01309785subject
Has Abstractpub_date
1993-01-01 00:00:00pages
15-27issue
1-2eissn
0304-8608issn
1432-8798journal_volume
128pub_type
杂志文章abstract::Bovine viral diarrhoea virus 1 (BVDV-1) and Bovine viral diarrhoea virus 2 (BVDV-2) are two recognised bovine pestivirus species of the genus Pestivirus. Recently, a pestivirus, termed SVA/cont-08, was detected in a batch of contaminated foetal calf serum originating from South America. Comparative sequence analysis s...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0419-4
更新日期:2009-01-01 00:00:00
abstract::Whilst bovine leukemia virus (BLV) causes considerable economic losses to the dairy industry worldwide, information on its molecular epidemiology and economic impact in beef cattle is limited. Here, blood from 880 animals from Zambia's major cattle-rearing provinces was screened for BLV by nested PCR. Positive pools w...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04350-6
更新日期:2019-10-01 00:00:00
abstract::Knowledge of coronaviruses in wild carnivores is limited. This report describes coronavirus genetic diversity, species specificity and infection prevalence in three wild African carnivores. Coronavirus RNA was recovered from fresh feces from spotted hyena and silver-backed jackal, but not bat-eared fox. Analysis of se...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1562-x
更新日期:2013-04-01 00:00:00
abstract::The C-terminal R peptide of ecotropic murine leukemia virus (MLV) envelope protein (Env) negatively controls membrane fusion activity. The R peptide cleavage during virion maturation activates its fusogenicity and is required for viral entry. We analyzed fusogenicity and transduction efficiency of mutant Env proteins ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-1054-6
更新日期:2007-01-01 00:00:00
abstract::We studied the kinetics of localisation of matrix (M) protein of Human respiratory syncytial virus (RSV) in infected cells. M protein was detected in the nucleus early in infection, by confocal microscopy and by immunoblotting of nuclear fractions. We next tested the possibility that M protein may be involved in inhib...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0112-y
更新日期:2003-07-01 00:00:00
abstract::In previous work, we demonstrated that the arenavirus Junín virus (JUNV) is able to activate Akt by means of the phosphatidylinositol-3-kinase (PI3K) survival pathway during virus entry. This work extends our study, emphasizing the relevance of this pathway in the establishment and maintenance of persistent infection ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2298-6
更新日期:2015-02-01 00:00:00
abstract::A total of 72 Borna Disease virus infected rabbits were treated with different concentrations of cyclophosphamide, glucocorticoids or both in combination. Comparison with untreated, infected rabbits showed a drastic alteration in the clinical picture, a considerable prolongation of the survival time, and differences i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318132
更新日期:1981-01-01 00:00:00
abstract::Cotton blue disease is an aphid-transmitted cotton disease described in Brazil in 1962 as Vein Mosaic "var. Ribeirão Bonito". At present it causes economically important losses in cotton crops if control measures are not implemented. The observed symptoms and mode of transmission have prompted researchers to speculate...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0475-8
更新日期:2005-07-01 00:00:00
abstract::Non-infectious hemagglutinating particles of rabies virus accumulated in the fluid phase of chick embryo cell cultures at 6 days post-infection, though they were undetectable at 4 days. They were characterized as looped filaments resembling viral envelope as revealed by electron microscopy. Another form of hemagglutin...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317937
更新日期:1976-01-01 00:00:00
abstract::Allexiviruses are economically important garlic viruses that are involved in garlic mosaic diseases. In this study, we characterized the allexivirus cysteine-rich protein (CRP) gene located just downstream of the coat protein (CP) gene in the viral genome. We determined the nucleotide sequences of the CP and CRP genes...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3749-2
更新日期:2018-06-01 00:00:00
abstract::Genomic information about Muscovy duck parvovirus is still limited. In this study, the genome of the pathogenic MDPV strain YY was sequenced. The full-length genome of YY is 5075 nucleotides (nt) long, 57 nt shorter than that of strain FM. Sequence alignment indicates that the 5' and 3' inverted terminal repeats (ITR)...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2946-0
更新日期:2016-09-01 00:00:00
abstract::The E5 protein of papillomaviruses is a strongly hydrophobic membrane protein that can associate with the 16 kDa protein subunit of the vacuolar proton ATPase in endosomes and the Golgi apparatus resulting in raise of intraorganelle pH. We demonstrate that E5 of human papillomavirus type 16 (HPV16) when transfected in...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050070048
更新日期:2000-01-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) causes a variety of cancers, including Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman disease (MCD). Host cellular microRNAs (miRNAs) play important post-transcriptional regulatory roles in gene expression and can greatly influence viru...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3478-y
更新日期:2017-11-01 00:00:00
abstract::Norovirus (NoV) infection is the most common cause of nonbacterial acute gastroenteritis, which affects both adults and children. However, the molecular epidemiology of NoV in adults with acute gastroenteritis in China has not been investigated extensively. In this study, we investigated the occurrence of NoV infectio...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2440-0
更新日期:2015-07-01 00:00:00
abstract::Transmission electron microscopy was used to confirm the presence of picorna-like virus particles presumed to be Homalodisca coagulata virus-1 (HoCV-1) in the midgut region of adult glassy-winged sharpshooters (GWSS). In addition, we offer a reverse transcription polymerase chain reaction (RT-PCR) assay for the detect...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-1066-2
更新日期:2008-01-01 00:00:00
abstract::Foot-and-mouth disease virus (FMDV) is the most contagious pathogen in cloven-hoofed (two-toed) animals. Due to the rapid replication and spread of FMDV, novel therapeutic strategies are greatly needed to reduce or block FMDV shedding in cases of disease outbreak. Here, we generated an IRES-Mx1 construct in which the ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2473-4
更新日期:2015-08-01 00:00:00
abstract::During the course of hominoid evolution, a new transcript variant of the GSDML (gasdermin-like protein) gene was formed by the integration of the antisense-oriented HERV-H (human endogenous retrovirus) LTR (long terminal repeat) element. To investigate regions that are critical for transcriptional regulation of the GS...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0105-y
更新日期:2008-01-01 00:00:00
abstract::117F and R are subgroup I and II cucumber mosaic virus (CMV) strains, respectively. Whereas I17F induces severe symptoms on all hosts so far tested, R induces generally mild symptoms, except on Nicotiana glutinosa, on which it causes leaf blistering and severe stunting. Pseudorecombinants and recombinants, based on RN...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050510
更新日期:1999-01-01 00:00:00
abstract::A 945 nucleotide region (bases 76-1020) of the HA1 part of the HA gene was obtained for 31 influenza viruses of H7 subtype isolated primarily from Europe, Asia and Australia over the last 20 years. These were analysed phylogenetically and compared with sequences of the same region from 23 H7 subtype viruses available ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050695
更新日期:2000-01-01 00:00:00
abstract::We investigated the frequency and the clinical relevance of hepatitis B virus (HBV) pre-S mutations in Qidong, China. The results showed HBV pre-S mutants were detected in 48.4% (47/97) of patients with HBV infection. Both pre-S deletion and pre-S2 start codon mutations were more frequently found in HCC than in CH pat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0176-9
更新日期:2008-01-01 00:00:00
abstract::Since serological donor-screening tests for HIV were introduced in 1985, the safety of donated blood components has improved dramatically. However, these tests do not completely prevent the risk of transfusion-associated HIV infection related to the use of blood donated during the pre-seroconversion window period. Tes...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1085-x
更新日期:2011-11-01 00:00:00
abstract::Mitochondrial movement and distribution throughout the cytoplasm is crucial for maintaining cell homeostasis. Mitochondria are dynamic organelles but can be functionally disrupted during infection. Here, we show that the ubiquitous human pathogens HHV-1 and HHV-2 induce changes in the mitochondrial morphology and dist...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3890-y
更新日期:2018-10-01 00:00:00
abstract::Manipulation of African swine fever virus (ASFV) genomes, in particular those from field strains, is still a challenge. We have shown recently that generation of a green-fluorescent-protein-expressing, thymidine-kinase-negative (TK-) mutant of the low-pathogenic African swine fever virus field strain NHV was supported...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2095-2
更新日期:2014-09-01 00:00:00
abstract::By reverse transcription (RT)-nested polymerase chain reaction (PCR), hepatitis E virus (HEV) RNA was simultaneously detected in the livers of aborted fetuses and in fecal and serum samples from their sows, obtained from two of 14 farms tested. Sequence and phylogenetic analyses showed that these HEVs belonged to geno...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0679-z
更新日期:2010-07-01 00:00:00
abstract::Isolates of bovine viral diarrhea (BVD) virus were differentiated by monoclonal antibodies (MoAbs) reactive with the 56 kD viral polypeptide. Patterns of neutralizing activity of the MoAbs indicate that multiple epitopes are involved in virus neutralization. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311029
更新日期:1988-01-01 00:00:00
abstract::In February 2008, five patients were infected with the H1N1 subtype of influenza A virus in one hospital ward for immunocompromised patients at a hospital in North Rhine-Westphalia, Germany. All of these patients had an established haematologic disease and tested positive either for viral RNA or antigen shortly after ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0771-4
更新日期:2010-11-01 00:00:00
abstract::The competition between different types of aphthoviruses (foot-and-mouth disease virus [FMDV]) for receptor site utilization was determined. The Southern African Territories (SAT) types of FMDV absorbed poorly to BHK-21 cells as measured by a radioactivity binding assay but grew to relatively high titers on these cell...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01320782
更新日期:1982-01-01 00:00:00
abstract::Semliki Forst Virus (SFV) infected chick embroyo cells (CEC) incubated in the presence of Tunicamyein, a drug which specifically inhibits glycosylation, were studied under the electron microscope. No extracellular virions were produced although intracellular viral nucleocapsids in paracrystalline form were detected. U...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314489
更新日期:1977-01-01 00:00:00
abstract::The betaherpesvirus human herpesvirus 6 (HHV-6) has two variants. The U83 gene product of strain HST is a chemoattractant for monocytes. Here, we describe U83 gene variations that accumulated in variants A and B. A gene-variation hot spot was examined in 36 different strains and one donor DNA sample. U83 gene variatio...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0307-3
更新日期:2009-01-01 00:00:00
abstract::The structure and organization of the large (L) RNA of iris yellow spot virus (IYSV) was determined, and with this report, the complete genomic sequence of IYSV of the genus Tospovirus, family Bunyaviridae has been elucidated. The L RNA of IYSV was 8,880 nucleotides in length and contained a single open reading frame ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0568-5
更新日期:2010-02-01 00:00:00