Antigenic comparison of swine influenza virus isolates.

Abstract:

:Cross hemagglutination inhibition (HI) and neuraminidase inhibition (NI) tests demonstrated strong antigenic relationship between A/Swine/Wisconsin/1/73 (SW/73) and A/Swine/Shope/15/31 (SW/31) influenza viruses. An eigthyone fold purification of virus was achieved by adsorption and elution followed by differential ultracentrifugation and sedimentation through linear sucrose gradient. Radioimmunoassay using purified 125I labeled viral antigens revealed antigenic variation between the two virus isolates. Neuraminidase of both viruses had pH optima between 6.5 and 7.0, and SW/31 enzyme was relatively more heat stable than SW/73.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Nath DM,Rodkey LS,Minocha HC

doi

10.1007/BF01317967

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

245-52

issue

3

eissn

0304-8608

issn

1432-8798

journal_volume

48

pub_type

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