Neutralization of different strains of murine cytomegalovirus (MCMV)-effect of in vitro passage.

Abstract:

:Two strains of mouse cytomegalovirus (MCMV) propagated in mouse salivary glands could be distinguished in neutralization tests. Cell (mouse embryo fibroblast)-passaged virus of either strain was generally 10-20 times easier to neutralize than virus produced in mouse salivary glands. Evidence was obtained that mouse immunoglobulin was attached to virus particles in salivary gland preparations. First, antibody to mouse immunoglobulin inactivated salivary gland virus but not cell culture-grown virus. Second, virus obtained from mice early after infection, before antibody production, was as easy to neutralize as cell culture-grown virus. The attached immunoglobulins were CMV-specific, non-neutralizing, and interfered with the action of neutralizing antibody. Non-neutralizing antibodies that bind to infectious virus particles are present in mouse serum at the time salivary glands are harvested, 3 weeks after infection.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Chong KT,Gould JJ,Mims CA

doi

10.1007/BF01315153

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

95-104

issue

2

eissn

0304-8608

issn

1432-8798

journal_volume

69

pub_type

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