Abstract:
:Since the first rescue of a recombinant Newcastle disease virus (rNDV) in the late 1990s, many more rNDVs have been rescued by researchers around the world. Regardless of methodology, the main principle behind rescue of the virus has remained the same, i.e., the formation of a functional replication complex by simultaneously providing the full-length viral RNA and the viral NP, P and L proteins. However, different strategies have been reported for the insertion of the full-length genome into a suitable transcription vector, which remains the most challenging step of the rescue. Moreover, several systems have been published for provision of the DNA-dependent RNA polymerase, which is needed for transcription of viral RNA (vRNA) from the transfected plasmid DNA. The aim of this article is to consolidate all of the current cDNA assembly strategies and transcription systems used in rescue of rNDV in order to attain a better understanding of the advantages and disadvantages of each approach.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Molouki A,Peeters Bdoi
10.1007/s00705-016-3065-7subject
Has Abstractpub_date
2017-01-01 00:00:00pages
1-12issue
1eissn
0304-8608issn
1432-8798pii
10.1007/s00705-016-3065-7journal_volume
162pub_type
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pub_type: 杂志文章
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更新日期:2017-04-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1007/s007050050090
更新日期:1997-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00705-013-1712-9
更新日期:2013-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00705-005-0626-6
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pub_type: 杂志文章
doi:10.1007/BF01347975
更新日期:1975-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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