Biological and molecular characterization of a highly divergent johnsongrass mosaic virus isolate from Pennisetum purpureum.

Abstract:

:The complete genome sequence (9,865 nucleotides) of a highly divergent johnsongrass mosaic virus isolate (JGMV-CNPGL) was determined using Illumina sequencing. This isolate infected 10 genotypes of gramineous plants including maize. A comparative analysis of the complete genome showed 80 % nucleotide (nt) sequence identity (86 % amino acid (aa) sequence identity) to a johnsongrass mosaic virus isolate from Australia. The coat protein (CP) identity values, however, were lower than those for the whole genome (78 % and 80 % for nt and aa, respectively) and were close to the species demarcation values (77 % nt and 80 % aa). Unexpectedly, the amino-terminal portion of CP of JGMV-CNPGL showed only 38 % sequence identity to other JGMV isolates. The biological implications of this sequence divergence remain to be elucidated.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Silva KN,Melo FL,Orílio AF,Nagata T,Silva MS,Fernandes CD,Fragoso RR,Dessaune SN,Resende RO

doi

10.1007/s00705-016-2832-9

subject

Has Abstract

pub_date

2016-07-01 00:00:00

pages

1981-6

issue

7

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-016-2832-9

journal_volume

161

pub_type

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