Abstract：
:The process of fusion from without (FFWO) induced by herpes simplex virus (HSV) was analyzed by using various inhibitors and compared to fusion from within (FFWI). The fate of certain elements of the cytoskeleton after FFWO was also investigated. Our experiments demonstrate FFWO as a very suitable system for study of early virus-cell interactions. Zn++ ions proved inhibitory for penetration whilst pretreatment of cells with Ca++ ions before infection enhanced FFWO activity. Dissociation of penetration from the fusion process itself was possible by use of Zn++ ions, low pH-treatment and antiserum on the one hand and N-ethylmaleimide and cytochalasin D on the other. Penetration itself needs only 6 min or less to proceed. FFWO is independent of inhibitors of glycosylation (tunicamycin) and intracellular vesicular traffic (monensin), protein-synthesis (cycloheximide) and energy-delivery (2.4 dinitrophenol and Na-azide). Analyzed strains of HSV-1 and -2 producing FFWI could be subgrouped into three categories: Strain ANG with high, strain HFEM and Lux with low and strains IES, Len, MP, US with no FFWO activity. The results of these experiments indicate that the property of FFWO is not purely a consequence of the number of PFU but depends on certain inherent properties of the virus particles. Addition of heparin as well as treatment of cells with heparitinase effectively prevented FFWO, indicating identical virus receptors for entrance of virus into cells and FFWO. During our studies several calf sera were found to inhibit FFWO-activity. Inhibition of FFWO by a glycoconjugate (ferritin coupled with oleic acid) indicates specific stereochemical hindrance of FFWO by this compound. Shortly after FFWO the actin filaments rearrange to form long fibres and surface fibronectin is being lost from the cell membrane.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Walev I,Wollert KC,Weise K,Falke Ddoi
10.1007/BF01310490subject
Has Abstractpub_date
1991-01-01 00:00:00pages
29-44issue
1-2eissn
0304-8608issn
1432-8798journal_volume
117pub_type
杂志文章abstract：:The nucleotide sequence of the granulin gene of Choristoneura fumiferana granulovirus (CfGV) was determined. The gene encodes a protein of 248 amino acids with a predicted Mr of 29.299 kDa. The granulin genes of Trichoplusia ni, Pieris brassicae and Cryptophlebia leucotreta granuloviruses showed homologies ranging fro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050181
更新日期：1997-01-01 00:00:00
abstract：:Infectious cDNA clones originally derived from a mild strain of Pepper mild mottle virus were constructed by replacing residue 649, a critical point for attenuation of this virus, with all possible amino acids. All clones were infectious to pepper plants and induced a variety of symptoms, including no visible symptoms...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0312-1
更新日期：2009-01-01 00:00:00
abstract：:Potato virus Y (PVY) infection has been a global challenge for potato production and the leading cause of downgrading and rejection of seed crops for certification. Accurate and timely diagnosis is a key for effective disease control. Here, we have optimized a reverse transcription loop-mediated amplification (RT-LAMP...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3635-3
更新日期：2018-02-01 00:00:00
abstract：:We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5' end of both RNA1...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1625-7
更新日期：2013-07-01 00:00:00
abstract：:Enterovirus 76 (EV76) is a new member of species Human Enterovirus A (HEV-A). So far, the only complete genome sequence of the prototype strain from France has been available. In this study, we determined the complete nucleotide sequence of strain 04360, isolated from an acute flaccid paralysis patient in Shandong pro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1067-z
更新日期：2011-09-01 00:00:00
abstract：:The DNA of mammalian cells treated with 4,5',8-trimethylpsoralen (Trioxsalen) is crosslinked (preventing transcription) only after photoactivation with near ultraviolet light. Suppression of gene action in cocultivated or fused cultures, including both induction and antiviral action of fibroblast interferon, can conse...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320762
更新日期：1980-01-01 00:00:00
abstract：:Porcine respiratory coronavirus (PRCV), a spike (S) gene deletion mutant of Transmissible gastroenteritis virus (TGEV), causes mild or subclinical respiratory infections in pigs. The shedding of PRCV/TGEV was studied at different days post-arrival in fecal and nasal swabs from PRCV/TGEV seronegative sentinel pigs intr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0245-z
更新日期：2004-05-01 00:00:00
abstract：:The aim of this study was to develop and evaluate a two-tube multiplex real-time RT-PCR assay for the detection and identification of four viral hemorrhagic fever (VHF) pathogens, severe fever with thrombocytopenia syndrome virus (SFTSV), Hantaan virus (HTNV), Seoul virus (SEOV), and dengue virus (DENV), from human cl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1677-8
更新日期：2013-09-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310981
更新日期：1987-01-01 00:00:00
abstract：:Segment 10, encoding nonstructural proteins 3 (NS3) and 3a (NS3a) of epizootic haemorrhagic disease virus serotype 1 (EHDV-1) was sequenced. Computer motif recognition programs were used for interpretation of the sequence data to predict a structure for NS3. Integral membrane protein theories were then applied to prod...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309968
更新日期：1995-01-01 00:00:00
abstract：:Here, we report a novel member of the genus Polerovirus, zucchini aphid-borne yellows virus (ZABYV), which was identified in zucchini grown for seed production in the Xinjiang Uygur Autonomous Region, China. The complete nucleotide sequence of the ZABYV genome was determined and found to be 5,792 nucleotides in length...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04217-w
更新日期：2019-08-01 00:00:00
abstract：:Serotype G14 was once considered to be uncommon among equine rotaviruses. While it sporadically emerged in some parts of the world, serotype G14 became the dominant G serotype among rotaviruses detected in foals with diarrhea in Japan in the late 1990s. However, it is not known how such recently emerging G14 rotavirus...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0967-3
更新日期：2003-05-01 00:00:00
abstract：:Mice are protected against lethal intraperitoneal and intravenous infection by encephalomyocarditis virus and Semliki Forest virus by sequential treatment with poly I followed by either polyC or poly5-hydroxyC without production of interferon when the treatments are 4 or more hours apart and by the intraperitoneal or ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318024
更新日期：1976-01-01 00:00:00
abstract：:The interaction between two different bovine respiratory syncytial virus (BRSV) strains and bovine alveolar macrophages (BAMs) was studied in vitro. Bovine respiratory syncytial virus replicated minimally in BAMs and most of the virus produced remained cell-associated. Approximately 1 out of 1,000 BAMs produced infect...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322681
更新日期：1995-01-01 00:00:00
abstract：:To provide information on the mechanism of perpetuation of influenza viruses among waterfowl reservoirs in nature, virological surveillance was carried out in Alaska during their breeding season in summer from 1991 to 1994. Influenza viruses were isolated mainly from fecal samples of dabbling ducks in their nesting pl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322743
更新日期：1995-01-01 00:00:00
abstract：:A Bayesian phylogenetic analysis of eight separate gene segments indicated A/Swine/Shandong/2/2003 (H5N1), A/Swine/Shandong/na/2003 (H9N2), A/Swine/Shandong/nb/2003 (H9N2) and A/Swine/Shandong/nc/2005 (H9N2) probably represent two multiple reassortant lineages, that had not been described before, with genes coming fro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1083-1
更新日期：2008-01-01 00:00:00
abstract：:Mouse embryo fibroblasts and mouse adrenal tumor cells support the replication of encephalomyocarditis (EMC) virus, whereas rat glial and rat hepatoma cells are non-permissive. These differences in susceptibility were not due to the lack of virus attachment to rat cells. The findings that rat cells could be transfecte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310884
更新日期：1986-01-01 00:00:00
abstract：:Salmonella phages SenALZ1 and SenASZ3, two novel phages infecting Salmonella enterica, were isolated and analyzed. The genomes of these two phages consist of 154,811 and 157,630 base pairs (bp), with G+C contents of 44.56% and 44.74%, respectively. Fifty-nine of 199 open reading frames (ORFs) in the SenALZ1 genome, an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04183-3
更新日期：2019-05-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1693-8
更新日期：2013-09-01 00:00:00
abstract：:We determined the complete nucleotide sequence of EDRD-1, a Japanese strain of the North American type-Porcine reproductive and respiratory syndrome virus (PRRSV), and identified a novel 117-base deletion and 108-base insertion previously reported in the nsp2 gene of the SP strain, which contains the largest genome am...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0098-6
更新日期：2008-01-01 00:00:00
abstract：:Infection of MS cells with African swine fever virus (ASFV) produces inhibition of protein synthesis which is detectable from 4.5 hours after infection. At least 34 viral polypeptides have been indentified with molecular weights ranging between 9500 and 243,000 daltons. Three of these proteins show affinity for the ce...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314980
更新日期：1980-01-01 00:00:00
abstract：:Molecular weights of six major polypeptides of infectious bronchitis virus (IBV) are: 1. 75,000; 2. 50,000; 3. 45,000; 4. 35,000; 5. 28,000 or 24,000, and 6. 22,000 dalton. According to the mobility of protein 5 the polypeptide patterns of IBV serotypes fall into two main categories. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315022
更新日期：1979-01-01 00:00:00
abstract：:The diagnosis of cytomegalovirus retinitis (CMV-R) is difficult and usually based on clinical criteria or invasive diagnostic procedures. The purpose of this study was to investigate a possible association between CMV-R and specific anti-CMV antibodies in tears. Paired tear and serum samples were obtained from 96 indi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0813-0
更新日期：2006-12-01 00:00:00
abstract：:An apparently single rotavirus A strain possessing a genotype constellation of G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 abruptly emerged, caused diarrhoea in children requiring hospitalisation, and increased to reach 27 % of strains detected during the first half of 2015 in Vietnam. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2682-x
更新日期：2016-02-01 00:00:00
abstract：:We detected a novel bovine hepacivirus N (HNV) subtype, IME_BovHep_01, in the serum of cattle in Tengchong, Yunnan, China, by high-throughput sequencing. The complete genome of IME_BovHep_01, was sequenced using an Illumina MiSeq sequencer and found to be 8850 nt in length, encoding one hypothetical protein. BLASTn an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04611-9
更新日期：2020-06-01 00:00:00
abstract：:The nucleo-, phospho- and matrix protein of measles virus were localized at high resolution within infected cells by use of post-embedding immunogold labelling techniques. In general, labelling with monospecific antibodies as well as with a polyvalent rabbit anti-measles hyperimmune antiserum revealed measles virus po...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311011
更新日期：1990-01-01 00:00:00
abstract：:Simultaneous infection of primary rabbit kidney cells with HSV type 1 TK+ and a TK- strain results in a mutual influence of both viruses on the induction of thymidine kinase (TK). TK+ virus has an enhancing and TK- virus a depressing effect on TK induction by a superinfecting TK+ virus. The enzyme induction depends on...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317531
更新日期：1975-01-01 00:00:00
abstract：:We determined the genome sequence of a new polerovirus that infects field pea and faba bean in China. Its entire nucleotide sequence (6021 nt) was most closely related (83.3% identity) to that of an Ethiopian isolate of chickpea chlorotic stunt virus (CpCSV-Eth). With the exception of the coat protein (encoded by ORF3...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1301-3
更新日期：2012-07-01 00:00:00
abstract：:11 African and two German IBDV strains isolated in the mid '80s from field outbreaks in vaccinated and unvaccinated chicken flocks displayed features of very virulent (vv) IBDV strains. The sequence data of the VP2 variable region and phylogenetic analysis confirm that these strains can be grouped within vv IBDV strai...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050009
更新日期：2000-01-01 00:00:00
abstract：:Among the four flavonoids tested quercetin and morin proved to be significantly effective against lethal Mengo virus-induced encephalitis in mice when the drugs were adminsitered orally (p.o.). With subcutaneous (s.c.) administration all four drugs failed to prevent mortality in the infected mice. Quercetin produced m...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315089
更新日期：1978-01-01 00:00:00
