Abstract：
:Potato virus Y (PVY) infection has been a global challenge for potato production and the leading cause of downgrading and rejection of seed crops for certification. Accurate and timely diagnosis is a key for effective disease control. Here, we have optimized a reverse transcription loop-mediated amplification (RT-LAMP) assay to differentiate the PVY O and N serotypes. The RT-LAMP assay is based on isothermal autocyclic strand displacement during DNA synthesis. The high specificity of this method relies heavily on the primer sets designed for the amplification of the targeted regions. We designed specific primer sets targeting a region within the coat protein gene that contains nucleotide signatures typical for O and N coat protein types, and these primers differ in their annealing temperature. Combining this assay with total RNA extraction by magnetic capture, we have established a highly sensitive, simplified and shortened RT-LAMP procedure as an alternative to conventional nucleic acid assays for diagnosis. This optimized procedure for virus detection may be used as a preliminary test for identifying the viral serotype prior to investing time and effort in multiplex RT-PCR tests when a specific strain is needed.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Treder K,Chołuj J,Zacharzewska B,Babujee L,Mielczarek M,Burzyński A,Rakotondrafara AMdoi
10.1007/s00705-017-3635-3subject
Has Abstractpub_date
2018-02-01 00:00:00pages
447-458issue
2eissn
0304-8608issn
1432-8798pii
10.1007/s00705-017-3635-3journal_volume
163pub_type
杂志文章abstract：:Two cell lines, CNS-5 and CNS-6, were established by cocultivation of sedimented cells in cerebrospinal fluid (CSF) from two anti-human T-lymphotropic virus type I (HTLV-I) antibody-positive male patients with encephalopathy and HTLV-I-associated myelopathy, respectively, with peripheral blood mononuclear cells from a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311306
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pub_type： 杂志文章
doi：10.1007/s00705-006-0801-4
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journal_title：Archives of virology
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doi：10.1007/s00705-007-0031-4
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311075
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journal_title：Archives of virology
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311054
更新日期：1990-01-01 00:00:00
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更新日期：2002-09-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0307-x
更新日期：2004-07-01 00:00:00
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更新日期：2017-10-01 00:00:00
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更新日期：2013-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311694
更新日期：1983-01-01 00:00:00
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journal_title：Archives of virology
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更新日期：2013-02-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310859
更新日期：1983-01-01 00:00:00
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更新日期：2007-01-01 00:00:00
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更新日期：2009-01-01 00:00:00
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更新日期：2008-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00705-013-1787-3
更新日期：2014-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-08-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0427-3
更新日期：2005-02-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04266-1
更新日期：2019-08-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：2008-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0330-y
更新日期：2004-10-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s705-002-8299-4
更新日期：2002-01-01 00:00:00
abstract：:The polypeptides associated with fowlpox virus (FPV) infection of chicken embryo skin (CES) cells were examined by metabolic labelling with [35S]-methionine and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Polypeptide synthesis was followed over the first 48 hours post infection, as this was s...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320959
更新日期：1987-01-01 00:00:00
