Abstract:
:Dengue virus (DENV) causes a life-threatening illness, with a wide range of symptoms from mild febrile illness, dengue fever (DF), to life-threatening illness, dengue hemorrhagic fever (DHF). Antibody-dependent enhancement (ADE) is considered to be a risk factor for DHF. In the present study, we determined the parameters for ADE assays using FcγR-expressing BHK cells. Monoclonal antibodies and human serum samples were used in the assays. We examined antibody concentration and virus concentration and analyzed whether antibody concentration or DENV-antibody ratio determines ADE activity. Virus growth was quantified by a conventional plaque titration method using FcγR-expressing BHK cells. The assay allowed the detection of DENV growth with inoculation doses ranging from 10(2) PFU/ml to 10(6) PFU/ml using monoclonal antibodies and undiluted or diluted serum samples. The results indicate that antibody concentration rather than DENV-antibody ratio determines the demonstration of ADE activity. Thus, antibody concentration rather than multiplicity of infection was defined as the main determinant in ADE assays using FcγR-expressing BHK cells.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Moi ML,Takasaki T,Saijo M,Kurane Idoi
10.1007/s00705-013-1787-3subject
Has Abstractpub_date
2014-01-01 00:00:00pages
103-16issue
1eissn
0304-8608issn
1432-8798journal_volume
159pub_type
杂志文章abstract::"Phages" include viruses of eubacteria and archaea. At least 5568 phages have been examined in the electron microscope since the introduction of negative staining in 1959. Most virions (96%) are tailed. Only 208 phages (3.7%) are polyhedral, filamentous, or pleomorphic. Phages belong to one order, 17 families, and thr...
journal_title:Archives of virology
pub_type: 杂志文章,评审
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abstract::The complete nucleotide sequence of the RNA genome of turnip mosaic virus Japanese strain (TuMV-J) has been determined from five overlapping cDNA clones and by direct sequencing of viral RNA. The RNA sequence was 9833 nucleotides in length, excluding a 3' terminal poly(A) tail. An AUG triplet at position 130-132 was a...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718209
更新日期:1996-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0184-8
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0226-3
更新日期:2008-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310768
更新日期:1991-01-01 00:00:00
abstract::R 61,837, a new antirhinovirus compound, was able to protect several susceptible rhinoviruses against inactivation by mild acidification or heat. This observation strengthens the hypothesis that the drug exerts antiviral activity by a direct interaction with the viral protein capsid to stabilize the particle. However,...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311037
更新日期:1989-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3478-y
更新日期:2017-11-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309371
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01487679
更新日期:1988-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2308-8
更新日期:2015-03-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311311
更新日期:1983-01-01 00:00:00
abstract::Our previous studies demonstrated that hepatitis E virus (HEV) requires the multivesicular body (MVB) pathway to release virus particles, suggesting that HEV utilizes the cellular ESCRT machinery in the cytoplasm, not at the cell surface, to be released from infected cells. In this study, we generated a murine monoclo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1912-3
更新日期:2014-05-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0838-y
更新日期:2002-08-01 00:00:00
abstract::Feline calicivirus (FCV) is a highly contagious pathogen that causes oral and upper respiratory tract disease in cats. Despite widespread vaccination, the prevalence of FCV remains high. Furthermore, a high gene mutation rate has led to the emergence of variants, and some infections are lethal. To date, there is no ef...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2534-8
更新日期:2015-12-01 00:00:00
abstract::Complementary oligonucleotide primers which flank a 1146-nucleotide gene fragment (S1B: nt 1185 to 2333) encompassing a polymorphic region (nt 1368 to 1776) of the S1 subunit of bovine coronavirus spike glycoprotein were used for enzymatic amplification by PCR. We chose four clinical isolates, recovered from cases of ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310017
更新日期:1994-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0113-x
更新日期:2003-08-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1280-4
更新日期:2012-06-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-1041-y
更新日期:2007-01-01 00:00:00
abstract::A study was made of the phylogenetic relationships between fifteen complete nucleotide sequences as well as 43 nucleotide sequences of the putative coat protein gene of different strains belonging to the virus species Beak and feather disease virus obtained from 39 individuals of 16 psittacine species. The species inc...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0368-x
更新日期:2004-12-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0885-4
更新日期:2002-12-01 00:00:00
abstract::A potyvirus (isolate PFV-FJ) infecting passion fruit in China was identified by small-RNA sequencing. The complete genome sequence of PFV-FJ was determined to be 9974 nucleotides, excluding the poly(A) tail. PFV-FJ shares 70-72% nucleotide and 69-74% amino acid sequence identity at the polyprotein level with seven rep...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04251-8
更新日期:2019-07-01 00:00:00
abstract::The combined use of phage and antibiotics can show synergistic antimicrobial effects, so-called phage-antibiotic synergy (PAS). Here, we screened and examined PAS against Pseudomonas aeruginosa in vitro. Testing four different phages infecting P. aeruginosa, phage KPP22 classified within the family Myoviridae genus Pb...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3811-0
更新日期:2018-07-01 00:00:00
abstract::The complete genome sequence (9,865 nucleotides) of a highly divergent johnsongrass mosaic virus isolate (JGMV-CNPGL) was determined using Illumina sequencing. This isolate infected 10 genotypes of gramineous plants including maize. A comparative analysis of the complete genome showed 80 % nucleotide (nt) sequence ide...
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pub_type: 杂志文章
doi:10.1007/s00705-016-2832-9
更新日期:2016-07-01 00:00:00
abstract::Avian influenza viruses of H3N8, H11N4, H7N7, H8N4, and H5N3 infected mink by contact. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309274
更新日期:1983-01-01 00:00:00
abstract::The biological function of entomopoxvirus (EPV) spindles (inclusion bodies that lack virions), has not been elucidated. We characterized the function of EPV spindles in the cupreous chafer, Anomala cuprea (Coleoptera: Scarabaeidae). Spheroids or spheroids mixed with spindles of Anomala cuprea EPV were administered per...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050070103
更新日期:2000-01-01 00:00:00
abstract::Sera and bronchial washings from normal mice and from mice previously infected with influenza virus were analyzed for their concentration of four immunoglobulin (Ig) classes by the method of single radial immunodiffusion and for their content of specific antibody of these Ig classes by the immunofluorescent method. Ig...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314846
更新日期:1977-01-01 00:00:00