Abstract：
:An active replication complex of citrus exocortis viroid (CEV) was isolated as a chromatin-enriched fraction of infected tomato leaf with CEV RNA synthesis activity. This activity was solubilised from the chromatin with ammonium sulphate, but not with sarkosyl. Nucleoprotein complexes in the soluble fraction which bound to a monoclonal antibody to the carboxy terminal domain of the largest subunit of RNA polymerase II (8WG16) were affinity purified and contained plus- and minus-sense CEV RNA. The results support a role for RNA polymerase II in viroid replication and provide the first direct evidence of an association in vivo between host RNA polymerase II and CEV.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Warrilow D,Symons RHdoi
10.1007/s007050050650keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
2367-75issue
12eissn
0304-8608issn
1432-8798pii
91442367.705journal_volume
144pub_type
杂志文章abstract：:Foot-and-mouth disease virus (FMDV) is the most contagious pathogen in cloven-hoofed (two-toed) animals. Due to the rapid replication and spread of FMDV, novel therapeutic strategies are greatly needed to reduce or block FMDV shedding in cases of disease outbreak. Here, we generated an IRES-Mx1 construct in which the ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2473-4
更新日期：2015-08-01 00:00:00
abstract：:The complete genome sequence (9,865 nucleotides) of a highly divergent johnsongrass mosaic virus isolate (JGMV-CNPGL) was determined using Illumina sequencing. This isolate infected 10 genotypes of gramineous plants including maize. A comparative analysis of the complete genome showed 80 % nucleotide (nt) sequence ide...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2832-9
更新日期：2016-07-01 00:00:00
abstract：:Three genetic variants of grapevine leafroll-associated virus 3 (GLRaV-3) were identified in vineyards of the Western Cape, South Africa. The GLRaV-3 variants were identified by single-strand conformation polymorphism (SSCP) profiles generated from a region amplified in ORF5. ORF5 sequence data confirmed the three gen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0793-y
更新日期：2010-12-01 00:00:00
abstract：:TT virus (TTV), the first human circovirus to be discovered, appears to be present in most people; less is known about the prevalence of the related TTV-like mini virus (TLMV). A sensitive nested PCR, specific for TLMV, detected the virus in 48% of 201 sera (Norwegian blood donors) previously found to have a 90% preva...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s705-002-8310-6
更新日期：2002-01-01 00:00:00
abstract：:The exact intracellular site of hepatitis A virus (HAV) production is unknown, possibly due to its usually slow and inefficient replication. Using immunocytochemistry and in-situ RT-PCR, we show that in cells infected with the rapidly replicating HAV strain HAS-15, viral proteins and RNA are scattered throughout the c...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170003
更新日期：2001-12-01 00:00:00
abstract：:The complement fixing antigens of rubella virus have been found to contain virion envelope but little nucleocapsid polypeptides. They are also shown to be associated with two additional polypeptides of host origin. Evidence is presented to suggest that the complement fixing antigens are structural components of the pl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315301
更新日期：1986-01-01 00:00:00
abstract：:The modes of molecular evolution of the coat protein (CP) and 3' non-coding region (NCR) were investigated in Bean yellow mosaic virus (BYMV) isolates, including a new pathotype from blue passion fruit. In phylogenetic analysis, the new pathotype did not cluster with pathotypes or host groups described previously. Int...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0485-7
更新日期：2009-01-01 00:00:00
abstract：:Tobacco vein banding mosaic virus (TVBMV) is of increasing importance in tobacco production. Knowledge of the genetic structure and variability of the virus population is vital for developing sustainable management. In this study, 24 new TVBMV isolates from Sichuan Province together with 46 previous isolates were stud...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04342-6
更新日期：2019-10-01 00:00:00
abstract：:Morphological, biological, serological, and molecular tests underpin the description of costus stripe mosaic virus (CoSMV) as a new member of the genus Potyvirus, family Potyviridae. Found affecting the native ornamental Costus spiralis in Brazil, the pathogen showed a severely restricted natural and experimental host...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04788-z
更新日期：2020-11-01 00:00:00
abstract：:Classic neutralization studies by Fazekas de St. Groth and Webster (8) on mixtures of influenza viruses and mixtures of rabbit antisera are reinterpreted in terms of a percentage contaminant in the stock used for the dilution series. A very small amount of a different virus changes the shape of quantal assay curves co...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317497
更新日期：1979-01-01 00:00:00
abstract：:Outbreaks of swine influenza were first observed in Japan in 1978. A number of influenza viruses were isolated from diseased swine. Almost all viruses isolated were swine influenza virus (Hsw 1 N1) but two viruses isolated from the nasal swabs of swine showing clinical signs of influenza in the Kanagawa prefecture wer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314741
更新日期：1980-01-01 00:00:00
abstract：:Human bocavirus (HBoV) is a new parvovirus associated with acute respiratory tract infection (ARTI). In order to evaluate HBoV significance as an agent of acute respiratory disease, we screened 1,135 respiratory samples from children and adults with and without symptoms during two complete calendar years. HBoV1 preval...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2238-5
更新日期：2015-01-01 00:00:00
abstract：:A virus isolated from sorghum in Nigeria has been partially characterized. It was tested by enzyme-linked immunosorbent assay (ELISA) using antisera to Maize dwarf mosaic virus, Johnsongrass mosaic virus (JGMV), Sugarcane mosaic virus strain-MDB, Sorghum mosaic virus, and Zea mosaic virus. A partial host range, sympto...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0411-y
更新日期：2005-03-01 00:00:00
abstract：:A potyvirus from Chinese narcissus was transmitted mechanically to three species of Narcissus and to Lycoris radiata but not to 22 other test species. In western blot, the coat protein reacted strongly with Narcissus degeneration virus (UK isolate) antiserum. Antiserum raised to the Chinese virus did not react with ei...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0841-9
更新日期：2007-02-01 00:00:00
abstract：:We investigated the frequency and the clinical relevance of hepatitis B virus (HBV) pre-S mutations in Qidong, China. The results showed HBV pre-S mutants were detected in 48.4% (47/97) of patients with HBV infection. Both pre-S deletion and pre-S2 start codon mutations were more frequently found in HCC than in CH pat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0176-9
更新日期：2008-01-01 00:00:00
abstract：:Dengue virus is the most important arbovirus that affects humans, and it can establish persistent infections, especially in insect-derived cell cultures. Defective viral genomes have been implicated in the establishment and maintenance of persistent infections with several flaviviruses; however, there exists almost no...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1525-2
更新日期：2013-03-01 00:00:00
abstract：:To provide information on the mechanism of attenuation of a Newcastle disease vaccine strain, TCND, we compared it with the parental virulent strain California 11,914 (CAL) biologically and genetically. It was found that TCND bore the fusion protein of virulent type, consisting of a pair of dibasic amino acid residues...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050361
更新日期：1998-01-01 00:00:00
abstract：:Analysis of the preS1 gene of hepatitis B virus was used to define two nosocomial outbreaks of HBV infection. In an outbreak in an oncology unit we had previously shown by single stranded conformational polymorphism (SSCP) analysis of a 189 bp fragment of the preS1 gene, that 52 children were infected with HBV strains...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050200
更新日期：1997-01-01 00:00:00
abstract：:A novel strain of Japanese yam mosaic virus (JYMV-CN) was identified in a yam plant with foliar mottle symptoms in China. The complete genomic sequence of JYMV-CN was determined. Its genomic sequence of 9701 nucleotides encodes a polyprotein of 3247 amino acids. Its organization is virtually identical to that of two J...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2231-z
更新日期：2015-02-01 00:00:00
abstract：:We have determined the complete nucleotide sequence of three isolates (two severe and one attenuated) of the potyvirus Leek yellow stripe virus (LYSV), the main causal agent of the garlic mosaic disease that is prevalent in Aomori prefecture, northern Japan. They contained 10,296-10,297 nucleotides and encoded a deduc...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0482-9
更新日期：2005-06-01 00:00:00
abstract：:To provide information on the mechanism of perpetuation of influenza viruses among waterfowl reservoirs in nature, virological surveillance was carried out in Alaska during their breeding season in summer from 1991 to 1994. Influenza viruses were isolated mainly from fecal samples of dabbling ducks in their nesting pl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322743
更新日期：1995-01-01 00:00:00
abstract：:Samples containing two viruses belonging to the genus Polerovirus, beet chlorosis virus (BChV) and beet mild yellowing virus (BMYV), were collected from French and Polish sugar beet fields. The molecular properties of 24 isolates of BChV and BMYV were investigated, and their genetic diversity was examined in the coat ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2245-6
更新日期：2015-02-01 00:00:00
abstract：:Brefeldin A (BFA) induced a rapid redistribution of vesicular stomatitis virus G protein (VSV-G) to the endoplasmatic reticulum (ER) in interferon (IFN)-pretreated cells. This result is consistent with accumulation of VSV-G in the trans-Golgi (GC) complex in cells pretreated with IFN and implies that IFN does not inte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314635
更新日期：1992-01-01 00:00:00
abstract：:Complete nucleotide (nt) and deduced amino acid sequences of two onion yellow dwarf virus (OYDV) isolates showing mild and severe symptoms in onion but being unable to infect garlic were determined. The genomes consisted of 10,459 and 10,461 nt (without the 3' poly(A) tail) and were 92.2 % identical. Comparison of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1597-z
更新日期：2013-06-01 00:00:00
abstract：:The objective of this study was to test the ability of bovine viral diarrhea virus (BVDV) to infect mice. Two mice each were either mock infected or inoculated with one of three BVDV strains by the intraperitoneal (IP) (n = 8) or intranasal (IN) (n = 8) route. All mice were euthanized at day 7 postinfection (p.i.). No...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2412-4
更新日期：2015-06-01 00:00:00
abstract：:Rice transitory yellowing virus (RTYV), a member of the genus Nucleorhabdovirus, is closely related to or synonymous with rice yellow stunt virus (RYSV). To clarify the relationship between RTYV and RYSV, we determined the nucleotide sequence of the RTYV genome. The RTYV genome consists of 14,029 nucleotides. The over...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0557-8
更新日期：2010-02-01 00:00:00
abstract：:A cross-sectional study was undertaken during 2012-2013 to determine the prevalence, strains and factors associated with rotavirus infection among under-5-year-old children hospitalized with acute diarrhea in Uganda. Rotaviruses were detected in 37 % (263/712) of the children. The most prevalent strains were G9P[8] (2...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2742-2
更新日期：2016-04-01 00:00:00
abstract：:The complete genome sequences of two grapevine virus L (GVL) isolates collected from the wine grape cultivar Blanc du Bois (Vitis spp.: 'Florida D 6-148'×'Cardinal') in Texas were determined. The two genome sequences (excluding the polyA tail) were each 7594 nucleotide long and 99.7% identical to each other, but they ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04682-8
更新日期：2020-08-01 00:00:00
abstract：:The recent H5N1 avian influenza outbreaks in Asia spread over more than 8 countries. It has caused enormous economic loss and grand challenges for the public health. During these breakouts we isolated three strains of H5N1 Avian Influenza Virus (AIV) from chickens and one from duck in different farms of Southern China...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0474-9
更新日期：2005-06-01 00:00:00
abstract：:Four epidemic seasons of rotaviruses were studied in Helsinki during 1986-1990. This is the first Scandinavian study, where both electropherotypes and serotypes are determined. Out of 5316 fecal specimens 769 (14.5%) rotavirus positive samples were detected by electron microscopy. Of these, 645 isolates (83.9%) gave a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314964
更新日期：1995-01-01 00:00:00
