Abstract:
:Human peripheral blood mononuclear cells (PBMC) can be infected in vitro with sera of hepatitis C virus (HCV)-infected patients. We have treated pools of PBMC with dextran sulfate MW 8,000, before infection with various HCV(+) sera. When the cells were treated with dextran sulfate 10-3 M, HCV RNA was no longer detectable after infection with all HCV genotypes tested. When the cells incubated with dextran sulfate 10-3 M and 10-4 M were maintained in culture for 4 weeks, no significant HCV replication could be observed. Our data suggest that dextran sulfate inhibits the attachment of HCV to the cell surface, since the HCV RNA detection was similar to control cells when dextran sulfate was added after infection.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Cribier B,Schmitt C,Kirn A,Stoll-Keller Fdoi
10.1007/s007050050293subject
Has Abstractpub_date
1998-01-01 00:00:00pages
375-9issue
2eissn
0304-8608issn
1432-8798journal_volume
143pub_type
杂志文章abstract::To eradicate rabies in foxes, almost 97 million oral rabies vaccine baits have been distributed in Germany and Austria since 1983 and 1986, respectively. Since 2007, no terrestrial cases have been reported in either country. The most widely used oral rabies vaccine viruses in these countries were SAD (Street Alabama D...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0408-7
更新日期:2009-01-01 00:00:00
abstract::Terminal sequences of the bi-segmented dsRNA genome of 3 different strains of infectious bursal disease virus (IBDV) were analyzed by the rapid amplification of cDNA 5' ends (5'RACE) procedure. Both segments are 85% homologous in a 32-nucleotide sequence comprising the 5' end, whereas the 3' end has a conserved pentam...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718616
更新日期:1996-01-01 00:00:00
abstract::Gp 85 the major envelope glycoprotein of Rous sarcoma virus can be released from intact by virus treatment with 2-mercaptoethanol. Attempts were made to reassociate released gp 85 to 2-mercaptoethanol treated virus. In order to study the biological activity and to analyse the reaction product with biochemical methods ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314883
更新日期:1982-01-01 00:00:00
abstract::Mouse cell lines (L, NIH3T3, and RMA cells) infected with the Edmonston strain of measles virus (MV) did not exhibit cytopathic effects (CPE), consistent with the finding that mice are not susceptible to MV. Northern blot analysis, however, revealed that MV genes were transcribed in infected L and NIH3T3 cells, althou...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310037
更新日期:1994-01-01 00:00:00
abstract::The plaque size and morphology of twenty-two influenza A virus recombinants representing seven distinct families were analyzed on MDCK cells. By examination of the genetic composition of the recombinants no relationship could be established between any gene, including those coding for the surface antigens, and the pla...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314716
更新日期:1982-01-01 00:00:00
abstract::The recent H5N1 avian influenza outbreaks in Asia spread over more than 8 countries. It has caused enormous economic loss and grand challenges for the public health. During these breakouts we isolated three strains of H5N1 Avian Influenza Virus (AIV) from chickens and one from duck in different farms of Southern China...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0474-9
更新日期:2005-06-01 00:00:00
abstract::Hepatitis B virus (HBV) infection has a major effect on health care systems, with about one-third of the world's population currently infected with the virus. There is an effective vaccine against HBV, which contains a recombinant "surface antigen" produced in an expression vector. Vaccination has proved to be success...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0206-7
更新日期:2008-01-01 00:00:00
abstract::To trace the possible route of introduction of porcine epidemic diarrhea virus (PEDV), the phylogenetic relationships of PEDV strains in the regions of epidemicity in the Philippines to PEDV strains that are endemic in other countries were investigated. Partial nucleotide sequences of the S1 spike gene was determined ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2938-0
更新日期:2016-09-01 00:00:00
abstract::Reverse transcription coupled with the polymerase chain reaction (RT-PCR) was used for the rapid laboratory diagnosis of pestivirus infections. A direct DNA sequencing method was developed for the analysis of the amplified cDNA from the 5' noncoding region of the viral genome. 70 pestivirus strains were compared in th...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309467
更新日期:1994-01-01 00:00:00
abstract::R 61,837, a new antirhinovirus compound, was able to protect several susceptible rhinoviruses against inactivation by mild acidification or heat. This observation strengthens the hypothesis that the drug exerts antiviral activity by a direct interaction with the viral protein capsid to stabilize the particle. However,...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311037
更新日期:1989-01-01 00:00:00
abstract::The full length gene for the coat protein (CP) of the potyvirus, Johnsongrass mosaic virus, was incorporated into recombinant baculovirus and expressed in insect cells. Western blot and Coomassie-stained polyacrylamide gel electrophoresis analysis of infected insect cells demonstrated that CP was produced in large qua...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01321065
更新日期:1994-01-01 00:00:00
abstract::We investigated the frequency and the clinical relevance of hepatitis B virus (HBV) pre-S mutations in Qidong, China. The results showed HBV pre-S mutants were detected in 48.4% (47/97) of patients with HBV infection. Both pre-S deletion and pre-S2 start codon mutations were more frequently found in HCC than in CH pat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0176-9
更新日期:2008-01-01 00:00:00
abstract::Respiratory syncytial virus (RSV) G glycoprotein mediates cell attachment through surface glycosaminoglycans (GAGs). Feldman et al. [10] suggested that specific basic amino acids in residues 184-198 of G defined a critical heparin binding domain (HBD). To further define the G HBD we made a series of truncated G protei...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0139-0
更新日期:2003-10-01 00:00:00
abstract::The receptor for adeno-associated virus serotype 2 (AAV2) in Purkinje cells has not been identified, but based on work carried out in non-neuronal cell lines, heparan sulfate proteoglycan is thought to act as a primary receptor, with basic fibroblast growth factor receptor-1 being reported as a co-receptor. In this st...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0222-7
更新日期:2008-01-01 00:00:00
abstract::Two non-virogenic Vero cell lines persistently infected with the arenavirus Junin (JUNV), named V3 and V7, were characterized with respect to their resistance to superinfection with homologous and antigenically related viruses. Both lines were refractory to JUNV multiplication and partially resistant to other arenavir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0227-1
更新日期:2004-03-01 00:00:00
abstract::Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317927
更新日期:1989-01-01 00:00:00
abstract::Outbreaks of swine influenza were first observed in Japan in 1978. A number of influenza viruses were isolated from diseased swine. Almost all viruses isolated were swine influenza virus (Hsw 1 N1) but two viruses isolated from the nasal swabs of swine showing clinical signs of influenza in the Kanagawa prefecture wer...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314741
更新日期:1980-01-01 00:00:00
abstract::HIV-1 genetic diversity information from a pediatric population is scarce. This study enrolled 128 children living with HIV/AIDS, 103 antiretroviral-treated and 25 naive, from the Sao Paulo metropolitan area. Gag, pol and env regions were amplified, and drug resistance mutations, V3 loop, tropism and viral clades were...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0178-7
更新日期:2008-01-01 00:00:00
abstract::The aim of this study was to develop and evaluate a two-tube multiplex real-time RT-PCR assay for the detection and identification of four viral hemorrhagic fever (VHF) pathogens, severe fever with thrombocytopenia syndrome virus (SFTSV), Hantaan virus (HTNV), Seoul virus (SEOV), and dengue virus (DENV), from human cl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1677-8
更新日期:2013-09-01 00:00:00
abstract::Lymphocytic chorimeningitis virus (LCMV), the prototype arenavirus, and Lassa virus (LASV), causative agent of Lassa hemorrhagic fever (LHF), belong to the Old World group of the family Arenaviridae. Both viruses have extensive strain diversity and significant variations in lethality and pathogenicity for man and expe...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0385-9
更新日期:2004-12-01 00:00:00
abstract::To evaluate the impact of new antiretroviral combinations (HAART: Highly Active Anti Retroviral Therapy) on HCV replication and liver enzyme levels, we analysed the changes in HCV viremia and aminotransferase levels in HIV and HCV co-infected patients. Moreover, to evaluate the influence of HCV infection on the effica...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050685
更新日期:2000-01-01 00:00:00
abstract::Serotype G14 was once considered to be uncommon among equine rotaviruses. While it sporadically emerged in some parts of the world, serotype G14 became the dominant G serotype among rotaviruses detected in foals with diarrhea in Japan in the late 1990s. However, it is not known how such recently emerging G14 rotavirus...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0967-3
更新日期:2003-05-01 00:00:00
abstract::An Indonesian very virulent (vv) strain of infectious bursal disease virus (IBDV), designated Tasik94, was characterised both in vivo and at the molecular level. Inoculation of Tasik94 into 5-week-old specific-pathogen-free (SPF) chickens resulted in 100% morbidity and 45% mortality. The complete nucleotide and predic...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0817-3
更新日期:2002-07-01 00:00:00
abstract::Porcine circovirus type 2 (PCV2) is a member of the genus Circovirus in the family Circoviridae. Most subgenomic molecules of PCV2 have been mapped. Here, the first full-length sequence of a subviral molecule of PCV2 (CH-IVT12) containing a reverse complement sequence of the PCV2 genome was determined by sequencing DN...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2859-y
更新日期:2016-07-01 00:00:00
abstract::A Bayesian phylogenetic analysis of eight separate gene segments indicated A/Swine/Shandong/2/2003 (H5N1), A/Swine/Shandong/na/2003 (H9N2), A/Swine/Shandong/nb/2003 (H9N2) and A/Swine/Shandong/nc/2005 (H9N2) probably represent two multiple reassortant lineages, that had not been described before, with genes coming fro...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-1083-1
更新日期:2008-01-01 00:00:00
abstract::Using a one-step model for cell-to-cell transmission of HIV infection we have identified two distinct phases of HIV RNA synthesis. The first phase (4 h-12 h p.i.) was marked by an increase in only the full-length 9 kb RNA, while the second phase (24 h p.i. onwards) comprised a significant increase in the levels of all...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050429
更新日期:1998-01-01 00:00:00
abstract::Had-2, a mouse mutant cell line derived from FM3A, constitutively releases interferon-alpha and beta and acquires resistance to Newcastle disease virus (NDV) and other viruses. However, Had-2 was found as susceptible to Sendai virus (HVJ) as FM3A. Even when Had-2 cells were infected simultaneously with NDV and HVJ, on...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718199
更新日期:1996-01-01 00:00:00
abstract::Papillomavirus capsid proteins L1 and L2 mediate virion attachment, internalization and trafficking. In our studies of the capsid proteins, we identified an interaction of L2 with the E3 ligase Smad ubiquitin regulatory factor 2 (Smurf2). Smurf2 expression alters BPV1 virion trafficking and L2 protein levels. Using BP...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-0924-0
更新日期:2011-05-01 00:00:00
abstract::Bovine leukemia virus (BLV), the etiologic agent of enzootic bovine leucosis, has caused pandemic outbreaks worldwide. Because transcription of the BLV is quickly blocked after infection, detecting integrated provirus at host genome is an important method of identifying whether an animal is infected. The aim of the pr...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2806-y
更新日期:2016-06-01 00:00:00
abstract::Antibody and cell-mediated immune responses were measured in rabbits immunized with live, UV inactivated herpes simplex virus or with a subunit vaccine containing envelope proteins. All the types of immunization procedures induced the production of antibody as well as a specific cellular immunity. Furthermore, the sub...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317862
更新日期:1976-01-01 00:00:00