Abstract：
:The nucleotide sequence of the human cytomegalovirus replication origin of strain Towne (an AatII-SacI fragment corresponding to nt 90372-94637 of strain AD169) was determined and compared with AD169. Two differences were found in the nucleotide sequence level. One was the alteration of structural organization (a major difference): a 189-bp region of AD169 (nt 93337-93525) was directly repeated three times in Towne. The other was a change in the nucleotide residue level including substitution, insertion, or deletion (a minor difference). The divergent residues were predominantly localized within the nt 92591-92855 region of AD169. A replication assay revealed that replication ability remained after deletion of the 189-bp repeat but disappeared after either a 1.5-kb deletion from the AatII end or a 0.9-kb deletion from the SacI end. The 1.5- and 0.9-kb regions were relatively conserved. These results indicate that at least two regions essential for replication ability lie outside of both the relatively variable region and the 189-bp repeat and suggest that these essential regions support replication even with a spatial separation of either one (AD169) or three repeats (Towne) of the 189-bp region.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Chen Z,Watanabe S,Yamaguchi Ndoi
10.1007/BF01718585subject
Has Abstractpub_date
1996-01-01 00:00:00pages
13-30issue
1eissn
0304-8608issn
1432-8798journal_volume
141pub_type
杂志文章abstract：:A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314377
更新日期：1977-01-01 00:00:00
abstract：:Human bocavirus (HBoV)-2, a new parvovirus, has been identified in stool samples and is suggested to be one of the etiologic agents of acute gastroenteritis (GE). The purpose of this study was to investigate the prevalence of HBoV-2 in children with GE. Stool samples were collected from 358 children hospitalized with ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0533-3
更新日期：2009-01-01 00:00:00
abstract：:Noroviruses, a major cause of acute gastroenteritis outbreaks worldwide, are constantly evolving. This ability is reflected in the speed and efficiency with which these viruses spread and remain in the human population. The present study reports the detection of a novel recombination event among norovirus genotypes in...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1428-2
更新日期：2012-12-01 00:00:00
abstract：:Phages, viruses targeting bacteria, have potential therapeutic applications in the control of infections with antibiotic-resistant bacteria. In this study, an Enterobacter cloacae phage, Ec_L1, was isolated from sewage sludge samples collected from a hospital. The genome of phage Ec_L1 consists of 51,894 bp with 48.24...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04672-w
更新日期：2020-08-01 00:00:00
abstract：:In this study, we isolated a mycobacteriophage infecting Mycobacterium smegmatis mc2155 from a soil sample collected in Shandong Province in China. This phage was named Shandong1. It is a member of the family Siphoviridae with an isometric head and a long tail. Its genome was found to be 60,618 bp long with 67.46% G +...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3534-7
更新日期：2017-12-01 00:00:00
abstract：:Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). We used microchip electrophoresis in combination with automatic image analysis to develop a novel high-throughput PCR-RFLP to type the gene sequences that encode BLV Tax 233. This method revealed that 233L-Tax is more prevalent than ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04842-w
更新日期：2020-12-01 00:00:00
abstract：:Zika virus (ZIKV) has been endemic in Southeast Asian countries for several years, but the presence of the virus has not been associated with significant outbreaks of infection unlike other countries around the world where the Asian lineage ZIKV was introduced recently. However, few studies have been undertaken using ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-04137-1
更新日期：2019-03-01 00:00:00
abstract：:Canine enteric coronaviruses (CCoVs) are important enteric pathogens of dogs. CCoVs with different variations are typically pantropic and pathogenic in dogs. In this study, we isolated a CCoV, designated HLJ-073, from a dead 6-week-old male Pekingese with gross lesions and diarrhea. Interestingly, sequence analysis su...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04296-9
更新日期：2019-08-01 00:00:00
abstract：:The stools of slaughtered pigs were screened for hepatitis E virus (HEV). HEV RNA was detected in 7.3% of the samples. HEV strains were characterized as genotype 3 subtype c, a cluster previously not described in Italy. These findings provide evidence that slaughterhouse workers may be exposed to HEV infection. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0544-0
更新日期：2010-01-01 00:00:00
abstract：:Classical swine fever (CSF), caused by the CSF virus (CSFV), is a highly contagious disease in pigs. In Korea, vaccination using a live-attenuated strain (LOM strain) has been used to control the disease. However, parenteral vaccination using a live-attenuated strain still faces a number of problems related to storage...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2182-4
更新日期：2014-12-01 00:00:00
abstract：:Cytomegalovirus (CMV) infections are common after transplantation, but usually successfully treated with antivirals. In this study, the detection of CMV-DNA in peripheral blood leukocytes was monitored and compared with CMVpp65-antigenemia in liver transplant patients receiving ganciclovir treatment. Twenty adult live...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0108-7
更新日期：2003-07-01 00:00:00
abstract：:Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique for the amplification of DNA under isothermal conditions. For the first time, we applied this method to develop a simple and sensitive tool for the detection of Pepino mosaic virus (PepMV). PepMV is an emerging pathogen that causes yield and qua...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1706-7
更新日期：2013-10-01 00:00:00
abstract：:Senecavirus A (SVA) is associated with vesicular disease in swine and the acute death of neonatal piglets. Here, senecavirus A was isolated from a pig displaying vesicular disease in Northeast China. The virus was designated as SVA/HLJ/CHA/2016 and its full-length nucleotide sequence was determined and analyzed in com...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3480-4
更新日期：2017-10-01 00:00:00
abstract：:The genomes of a large number of highly diverse novel circular DNA viruses from a wide range of sources have been characterised in recent years, including circular single-stranded DNA (ssDNA) viruses that share similarities with plant-infecting ssDNA viruses of the family Geminiviridae. Here, we describe six novel cir...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2358-6
更新日期：2015-05-01 00:00:00
abstract：:Exposure of human lung fibroblasts to human cytomegalovirus (HCMV) stimulated a rapid increase in the release of [3H] from cells prelabelled with radiolabelled arachidonic acid ([3H]AA). Maximum stimulation of [3H] release was observed at 20 min postinfection and was quantitatively similar to that induced by the tumor...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316678
更新日期：1990-01-01 00:00:00
abstract：:We discovered a soybean mosaic virus (SMV) variant (4278-1) that caused systemic infections in Nicotiana benthamiana plants, resulting in stem stunting and leaf shriveling. The virus had a particle morphology and incubation period similar to those of other SMV isolates but differed from them in the leaf symptoms it ca...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3123-1
更新日期：2017-02-01 00:00:00
abstract：:We previously reported the isolation of South River virus (SORV) from a pool of mosquitoes collected in the Yucatan Peninsula of Mexico (Farfan-Ale et al. in Vector Borne Zoonotic Dis 10:777-783, 5). The isolate (designated SORV-252) was identified as SORV after a 197-nucleotide region of its small RNA genome segment ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1280-4
更新日期：2012-06-01 00:00:00
abstract：:A simple radial method based on the formerly described paper disc technique was developed for demonstration of neutralizing antibodies to enteroviruses with plaque forming ability. Sera administered by 5 microliter wire-loops to wells cut into the agar overlay of virus infected cell monolayers produced radial neutrali...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317320
更新日期：1980-01-01 00:00:00
abstract：:Human bocavirus (HBoV) is a new parvovirus associated with acute respiratory tract infection (ARTI). In order to evaluate HBoV significance as an agent of acute respiratory disease, we screened 1,135 respiratory samples from children and adults with and without symptoms during two complete calendar years. HBoV1 preval...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2238-5
更新日期：2015-01-01 00:00:00
abstract：:Noninfectious virus particles were produced in Ehrlich ascites tumor cells infected intraperitoneally with fowl plague virus. The PFU yield of virus per cell was less than 0.1 and the ratio PFU/HA units in the progeny virus was less than 10(3). The virus particles had the same morphology and size as egg-grown virus bu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315278
更新日期：1978-01-01 00:00:00
abstract：:Among the tick-borne orbiviruses (genus Orbivirus, family Reoviridae), 36 serotypes are currently classified within a single virus species, Great Island virus. In this study, we report the first characterization of a tick-borne orbivirus isolated from the tick Ixodes turdus in Japan, which we identified as a new membe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2588-7
更新日期：2015-12-01 00:00:00
abstract：:Vanilla necrosis potyvirus (VNV) is the cause of significant losses to the South Pacific islands vanilla crop. The gene for the coat protein of VNV has been cloned and sequenced. Comparison of this gene with other potyviral coat protein sequences revealed 97% nucleotide sequence homology (98% amino acid homology) to a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316887
更新日期：1993-01-01 00:00:00
abstract：:Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profil...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04832-y
更新日期：2020-12-01 00:00:00
abstract：:The present study on genetic diversity of human papillomaviruses in women infected by HIV in Brazil describes the frequency, the genotypes, and five new variants of HPV. One hundred fifty cervical smears of HIV-positive women were subjected to cytological examination, and the DNA samples obtained were assayed by MY09/...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0828-6
更新日期：2007-01-01 00:00:00
abstract：:A 945 nucleotide region (bases 76-1020) of the HA1 part of the HA gene was obtained for 31 influenza viruses of H7 subtype isolated primarily from Europe, Asia and Australia over the last 20 years. These were analysed phylogenetically and compared with sequences of the same region from 23 H7 subtype viruses available ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050695
更新日期：2000-01-01 00:00:00
abstract：:Previously, we identified serological immunodeterminants of African swine fever virus (ASFV), including pK205R and pB602L, without homologues in the database. pK205R is expressed as a 33-kD protein from 4 h post-infection onward, initially diffusely distributed throughout cells, and subsequently in viral factories. pK...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0246-z
更新日期：2008-01-01 00:00:00
abstract：:Serology for parvovirus B19 has been hampered by limited availability of antigen which has often had to be isolated from viraemic blood donations. We have determined the sequence of the genome of one such isolate (Stu). It is 99% similar to the sequences of two other isolates (Wi and Au) except at the far 5'-end, wher...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718833
更新日期：1996-01-01 00:00:00
abstract：:The in vitro cytopathology and the neurovirulence to inbred mice demonstrated by five variants originally derived from one laboratory strain (Miyama) of type 1 herpes simplex virus (HSV-1) were studied comparatively. Three of the variants are syncytial [+GC (LPV), +GC (SPV), +GC (81)] and two are non-syncytial [-GCr a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317369
更新日期：1986-01-01 00:00:00
abstract：:The present study was conducted to identify avian reovirus (ARV) proteins that can activate the phosphatidylinositol 3-kinase (PI3K)-dependent Akt pathway. Based on ARV protein amino acid sequence analysis, σA, σNS, μA, μB and μNS were identified as putative proteins capable of mediating PI3K/Akt pathway activation. T...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2908-6
更新日期：2016-08-01 00:00:00
abstract：:Function of rotavirus NSP1 was analyzed by using single-NSP1 gene-substitution reassortants, SKF, SDF, and SNF which have the NSP1 gene derived from human rotaviruses KU, DS-1, and canine rotavirus K9, respectively, in the genetic background of simian rotavirus SA11. The NSP1 genes from KU, DS-1, K9, and SA11 exhibite...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050600
更新日期：1999-01-01 00:00:00
