Abstract：
:The nucleotide sequence of the human cytomegalovirus replication origin of strain Towne (an AatII-SacI fragment corresponding to nt 90372-94637 of strain AD169) was determined and compared with AD169. Two differences were found in the nucleotide sequence level. One was the alteration of structural organization (a major difference): a 189-bp region of AD169 (nt 93337-93525) was directly repeated three times in Towne. The other was a change in the nucleotide residue level including substitution, insertion, or deletion (a minor difference). The divergent residues were predominantly localized within the nt 92591-92855 region of AD169. A replication assay revealed that replication ability remained after deletion of the 189-bp repeat but disappeared after either a 1.5-kb deletion from the AatII end or a 0.9-kb deletion from the SacI end. The 1.5- and 0.9-kb regions were relatively conserved. These results indicate that at least two regions essential for replication ability lie outside of both the relatively variable region and the 189-bp repeat and suggest that these essential regions support replication even with a spatial separation of either one (AD169) or three repeats (Towne) of the 189-bp region.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Chen Z,Watanabe S,Yamaguchi Ndoi
10.1007/BF01718585subject
Has Abstractpub_date
1996-01-01 00:00:00pages
13-30issue
1eissn
0304-8608issn
1432-8798journal_volume
141pub_type
杂志文章abstract：:Since the first rescue of a recombinant Newcastle disease virus (rNDV) in the late 1990s, many more rNDVs have been rescued by researchers around the world. Regardless of methodology, the main principle behind rescue of the virus has remained the same, i.e., the formation of a functional replication complex by simulta...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-016-3065-7
更新日期：2017-01-01 00:00:00
abstract：:To investigate the genetic diversity of potato virus M (PVM; genus Carlavirus, family Betaflexiviridae), the complete nucleotide sequence of the coat protein gene of 30 PVM isolates from a major potato-growing region in Iran were determined. Phylogenetic analysis of these Iranian PVM isolates together with those avail...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2037-z
更新日期：2014-08-01 00:00:00
abstract：:Adult Balb/c mice were passively immunized with monoclonal antibodies (100 micrograms/mouse) raised against tick-borne encephalitis (TBE) virus then challenged 24 hours later s.c. with 10 LD50 of TBE virus (Nëudorfl isolate). None of the mice showed evidence of premature death although all except one of the monoclonal...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310983
更新日期：1987-01-01 00:00:00
abstract：:The nature of SRV-4 infection in cynomolgus macaques remains unclear to date. Here, we report the monitoring of 24 cynomolgus monkeys that were naturally infected with SRV-4 for virus isolation, proviral load and antibody. The results indicated that the SRV-4 antibody status was statistically correlated to environment...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1068-y
更新日期：2011-11-01 00:00:00
abstract：:The RNAs of 4 avian rotavirus isolates were analysed by polyacrylamide gel electrophoresis. One of these isolates was made from chickens, the other three were from turkeys. The RNAs were separated into 8 or 9 bands consisting of 11 segments, ranging in molecular weight from 0.24 X 10(6) to 2.09 X 10(6). In contrast to...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320765
更新日期：1980-01-01 00:00:00
abstract：:A potyvirus from Chinese narcissus was transmitted mechanically to three species of Narcissus and to Lycoris radiata but not to 22 other test species. In western blot, the coat protein reacted strongly with Narcissus degeneration virus (UK isolate) antiserum. Antiserum raised to the Chinese virus did not react with ei...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0841-9
更新日期：2007-02-01 00:00:00
abstract：:Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are two closely related alphaherpesviruses. BoHV-1 causes several syndromes in cattle, including respiratory disease and sporadic cases of encephalitis, whereas BoHV-5 is responsible for meningoencephalitis in calves. Although both viruses are neurotropic, they differ...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-019-04494-5
更新日期：2020-02-01 00:00:00
abstract：:Our previous studies demonstrated that hepatitis E virus (HEV) requires the multivesicular body (MVB) pathway to release virus particles, suggesting that HEV utilizes the cellular ESCRT machinery in the cytoplasm, not at the cell surface, to be released from infected cells. In this study, we generated a murine monoclo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1912-3
更新日期：2014-05-01 00:00:00
abstract：:Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major anti...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070087
更新日期：2000-01-01 00:00:00
abstract：:A number of avian avulavirus 1 (AAvV 1) isolates have been reported from avian and non-avian hosts worldwide with varying clinical consequences. In this regard, robust surveillance coupled with advanced diagnostics, genomic analysis, and disease modelling has provided insight into the molecular epidemiology and evolut...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04276-z
更新日期：2019-07-01 00:00:00
abstract：:A virus isolated from sorghum in Nigeria has been partially characterized. It was tested by enzyme-linked immunosorbent assay (ELISA) using antisera to Maize dwarf mosaic virus, Johnsongrass mosaic virus (JGMV), Sugarcane mosaic virus strain-MDB, Sorghum mosaic virus, and Zea mosaic virus. A partial host range, sympto...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0411-y
更新日期：2005-03-01 00:00:00
abstract：:In the Mt. Kosciusko alpine area of Australia there are three well-separated populations of Cardamine lilacina, an endemic sward-forming perennial brassica, and these are infected with turnip yellow mosaic tymovirus. The genetic variation in these viral populations has been assessed by an RNA hybrid mismatch polymorph...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309845
更新日期：1993-01-01 00:00:00
abstract：:The base sequences of the coding region of the nucleoprotein (NP) genes of two H 10 influenza A viruses, one avian (virus N) and one mink virus, have been determined by primer extension. When the NP genes and the NP sequences derived from the only open reading frame of the two H 10 viruses were compared with those of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01319816
更新日期：1988-01-01 00:00:00
abstract：:Here, we infected mice with cytopathic bovine viral diarrhea virus 1 (cp BVDV1) by oral inoculation and investigated the effects of infection by histopathological, immunohistochemical (IHC), hematological methods. Twelve mice were infected, and samples were obtained at day 2, 5, and 9 postinfection (pi). Most of the i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2649-y
更新日期：2016-02-01 00:00:00
abstract：:Alfamo- and ilarviruses are characterized by the deficiency of their genomes (three messenger-sense RNAs) to start an infection cycle. The RNAs are in capsids built from a single species of protein of about 24 kD. A few dimers of this coat protein per RNA molecule are sufficient to activate the genome. Since the first...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s007050050551
更新日期：1999-01-01 00:00:00
abstract：:In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and I...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04737-w
更新日期：2020-10-01 00:00:00
abstract：:Ninety four percent of the genome of bovine herpesvirus 4 (BHV-4) strain DN 599 was cloned and a physical map was constructed by Southern blot analysis using a library of cloned fragments cleaved with the 3 restriction enzymes (Eco RI, Bam HI, and Hin dIII). The genome length was estimated to be 156.5 kbp +/- 0.7. The...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309576
更新日期：1992-01-01 00:00:00
abstract：:Papaya sticky disease ('meleira') was first observed in Brazil at the beginning of the 1980s. The disease is characterized by intense latex exudation from the fruit surface that becomes dark as it oxidizes, which makes it difficult to sell. The causal agent, which has been called papaya meleira virus (PMeV), has been ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2605-x
更新日期：2015-12-01 00:00:00
abstract：:Establishment of a persistent infection of Spodoptera frugiperda nuclear polyhedrosis virus (NPV) in Spodoptera frugiperda (S.f.) cells occurred in three phases: the first phase was characterised by high levels of cell infection and death, the second phase by decreasing cell infection levels leading to the final phase...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310859
更新日期：1983-01-01 00:00:00
abstract：:Avian influenza viruses of H3N8, H11N4, H7N7, H8N4, and H5N3 infected mink by contact. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309274
更新日期：1983-01-01 00:00:00
abstract：:Anti-human T-lymphotropic virus type I/II (HTLV-I/II) antibodies were screened by particle agglutination test in a total of 66 patients with thalassemia major who received multiple transfusion from paid donors at the Blood Transfusion Hematology Center of Ho Chi Minh City in South Vietnam. HTLV-II infection was confir...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050170
更新日期：1997-07-01 00:00:00
abstract：:The complete nucleotide sequence of Pelargonium flower break virus (PFBV) has been determined. The genomic RNA is 3923 nucleotides (nt) long and contains five open reading frames (ORFs). The 5'-proximal ORF encodes a 27 kDa protein (p27) and terminates with an amber codon which may be read-through into an in-frame p56...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0231-5
更新日期：2004-03-01 00:00:00
abstract：:Few reports are available on HCV molecular epidemiology among IDUs in Eastern Europe, and none in Montenegro. The aim of this study was to investigate the HCV genotype distribution in Montenegro among IDUs and to perform Bayesian and evolutionary analysis of the most prevalent HCV genotype circulating in this populati...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3224-5
更新日期：2017-06-01 00:00:00
abstract：:Wild plants of the family Convolvulaceae are hosts for a few New World begomoviruses (genus Begomovirus, family Geminiviridae). In this work, we report the complete genome sequence of a new begomovirus infecting the wild convolvulaceous plant Jacquemontia tamnifolia in Venezuela. The cloned bipartite genome showed the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3372-7
更新日期：2017-08-01 00:00:00
abstract：:The deletion of the thymidine kinase (TK) gene of herpesviruses causes a reduction in their virulence. However, the effects of the TK gene in infectious laryngotracheitis virus (ILTV) have not been clearly elucidated. In the present study, we constructed a TK gene-deleted recombinant ILTV expressing the green fluoresc...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-001-0794-y
更新日期：2002-05-01 00:00:00
abstract：:In our attempt to isolate mutant cell lines resistant to Newcastle disease virus (NDV) we developed an improved procedure for enrichment of NDV-resistant cells from mouse FM3A cells and isolated a novel NDV-resistant mutant cell line, Had-2, with characteristics different from Had-1, a previously reported NDV-receptor...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310796
更新日期：1994-01-01 00:00:00
abstract：:Infections with influenza and respiratory syncytial virus (RSV) rank high among the most common human respiratory diseases worldwide. Previously, we developed a replication-incompetent influenza virus by replacing the coding sequence of the PB2 gene, which encodes one of the viral RNA polymerase subunits, with that of...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1932-z
更新日期：2014-05-01 00:00:00
abstract：:Rabies virus (RABV) isolates from two species of canids and three species of bats were analyzed by comparing the C-terminal region of the G gene and the G-L intergenic region of the virus genome. Intercluster identities for the genetic sequences of the isolates showed both regions to be poorly conserved. Phylogenetic ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0671-7
更新日期：2010-06-01 00:00:00
abstract：:Group C rotavirus (GCRV) infection has been described in several parts of the world, predominantly as sporadic cases of acute gastroenteritis. Little is known about the yearly changes in the GCRV strains from diarrheal outbreaks. Stool samples collected from outbreaks of acute gastroenteritis in Yokohama, Japan, betwe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1894-1
更新日期：2014-04-01 00:00:00
abstract：:Twenty-eight Belgian field isolates of bovine herpesvirus 4 (BHV-4) coming from a variety of clinical diseases have been studied by restriction analysis and Southern blot hybridization. The unique central part of the genome was very well conserved among strains; only one variation in a restriction site was detected in...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01319227
更新日期：1991-01-01 00:00:00
