qPCR assay for the detection of pseudocowpox virus.

Abstract:

:Pseudocowpox is a zoonosis caused by pseudocowpox virus (PCPV), which mainly affects cows but can be an occupational disease of humans. The aim of the study was to validate a quantitative polymerase chain reaction (qPCR) assay for the detection of PCPV. The assay was able to detect up to 1000 copies of PCPV per µL in field samples, with a sensitivity of 80% and a specificity of 100%. We did not observe any cross-reactivity between PCPV-positive samples and samples that were positive for other genetically similar viruses. The repeatability and reproducibility were adequate according to parameters preestablished in official test validation manuals.

journal_name

Arch Virol

journal_title

Archives of virology

authors

de Oliveira Lopes GA,Ferreira LR,de Souza Trindade G,Fonseca AA Jr,Dos Reis JKP

doi

10.1007/s00705-020-04872-4

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

243-247

issue

1

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-020-04872-4

journal_volume

166

pub_type

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