Abstract：
:Blot hybridization analysis of infected bovine herpesvirus 1 (BHV-1) cellular RNA isolated at various times post infection and after treatment with specific metabolic inhibitors was used to characterize transcription of the BHV-1 Cooper isolate. Synthesis of BHV-1 RNA was detected as early as 3 h post infection and reached a maximum at six to eight hours post infection. The most transcriptionally active area of the genome was between map units 0.110 to 0.195, within the HindIII I fragment. From the entire genome a total of 59 transcripts ranging in size from approximately 0.6 to 10 kilobases were characterized as belonging to one of three distinct classes. Using the protein synthesis inhibitor cycloheximide, three immediate-early transcripts were identified as originating from the internal inverted repeat region between map units 0.734 and 0.842, corresponding to the HindIII D fragment. Using phosphonoacetic acid to prevent virus DNA synthesis by inhibition of the BHV-1 DNA polymerase, 28 early transcripts were recognized. The remaining 28 transcripts, classified as late RNA, were detected without the use of metabolic inhibitors at 6 to 8 h post infection. Transcription of early and late RNA was not restricted to any specific area of the genome. Eighty percent of the transcripts from both the HindIII A fragment, between map units 0.381 to 0.537 within the unique long segment, and the HindIII K fragment, between map units 0.840 to 0.907 of the unique short segment, were designated as belonging to the early class.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Seal BS,Irving JM,Whetstone CAdoi
10.1007/BF01316744subject
Has Abstractpub_date
1991-01-01 00:00:00pages
55-73issue
1-4eissn
0304-8608issn
1432-8798journal_volume
121pub_type
杂志文章abstract：:Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. In 2013, a large outbreak was reported on the archipelago of French Polynesia. In this study, we report the detection and molecular characterization of Zika virus for the first time in Chile from an outbreak among the inhabitant...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2695-5
更新日期：2016-03-01 00:00:00
abstract：:At necropsy, several dogs which died showing symptoms of hemorrhagic diarrhea, had significant lesions of the mucosa that were found especially in the duodenum and upper part of the small bowel. Study of ultrathin sections from the diseased mucosa revealed particles resembling parvoviruses in altered nuclei of cells o...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320586
更新日期：1979-01-01 00:00:00
abstract：:The precipitating and neutralizing activities of normal bovine sera with FMDV were studied and compared. Twenty-two out of 79 normal bovine sera gave a positive reaction in micro neutralization tests with FMDV type O, while six did so with type A. In RID tests 32 sera were positive with type O and 28 with type A virus...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315533
更新日期：1978-01-01 00:00:00
abstract：:Members of the family Closteroviridae have emerged as a major problem in agricultural crops in the past two decades. Diodia vein chlorosis virus (DVCV) is an understudied whitefly-transmitted closterovirus. Given the presence of the primary host for the virus in major agricultural production areas in the United States...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1055-3
更新日期：2011-11-01 00:00:00
abstract：:Mouse cell lines (L, NIH3T3, and RMA cells) infected with the Edmonston strain of measles virus (MV) did not exhibit cytopathic effects (CPE), consistent with the finding that mice are not susceptible to MV. Northern blot analysis, however, revealed that MV genes were transcribed in infected L and NIH3T3 cells, althou...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310037
更新日期：1994-01-01 00:00:00
abstract：:The rate of evolution of the human papillomavirus 16 (HPV16) genome is low. However, the ability of the E6 oncoprotein to interact with distinct p53 variants causes selective pressure on the E6 gene. In addition, intratypic recombination events in the HPV16 E6 and E7 genes have been characterized as extraordinary phen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1555-9
更新日期：2013-04-01 00:00:00
abstract：:The high prevalence (14.3%) of HIV/HBV co-infections reported in west China makes it necessary to screen concurrent HBV infection in HIV carriers. HBV B genotype was shown to be dominant in 54 cases of HIV/HBV co-infection, accounting for 81.48% of the total. The total drug resistance rate observed was 3.70%. A1762T, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3900-0
更新日期：2018-10-01 00:00:00
abstract：:Treatment of Cricket paralysis virus infected Drosophila cells with iodoacetamide before radiolabelling with 35S-methionine results in the appearance of two high molecular weight polypeptides of approximately equal to 200,000 molecular weight, not apparent in untreated infected cells (17). To attempt to differentiate ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320788
更新日期：1981-01-01 00:00:00
abstract：:Oncogene-containing retroviruses are generated by recombination events between viral and cellular sequences, a phenomenon called "oncogene capture". The captured cellular genes, referred to as "v-onc" genes, then acquire new oncogenic properties. We report a novel feline leukemia virus (FeLV), designated "FeLV-AKT", t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3192-1
更新日期：2017-04-01 00:00:00
abstract：:The gene coding for the nucleocapsid protein NP of the influenza A virus recombinant strain 413 1,1 was characterized biochemically by molecular hybridization and fingerprint analysis. The data presented suggest that this NP gene has evolved by intracistronic recombination between NP genes of virus N and the fowl plag...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322701
更新日期：1980-01-01 00:00:00
abstract：:The genomes of all replication-competent retroviruses contain cis-acting elements that regulate gene expression. However, the identities of many of these elements remain to be characterized. Inserting sequences into the 3' untranslated region of a replication-competent spleen necrosis virus (SNV) vector disrupted its ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050486
更新日期：1999-01-01 00:00:00
abstract：:Hepatitis B virus (HBV) infection has a major effect on health care systems, with about one-third of the world's population currently infected with the virus. There is an effective vaccine against HBV, which contains a recombinant "surface antigen" produced in an expression vector. Vaccination has proved to be success...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0206-7
更新日期：2008-01-01 00:00:00
abstract：:The entry modes of Japanese encephalitis (JE) and dengue-2 (DEN-2) viruses into C6/36 mosquito cells and of DEN-2 virus into human peripheral blood monocytes in vitro were studied. Inoculation of either JE or DEN-2 virions into C6/36 cells resulted in direct penetration of the virions into the cytoplasm at the cell su...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313814
更新日期：1989-01-01 00:00:00
abstract：:The complete genome sequence of grapevine Bulgarian latent virus (GBLV) has been determined. RNA-1 (7,452 nt in length) contains a single ORF of 6,285 nt, encoding a polyprotein with conserved motifs characteristic of the viral protease cofactor (Prot-cofact), the NTP-binding protein (NTP), the cysteine-like protease ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0908-5
更新日期：2011-05-01 00:00:00
abstract：:The resistance of transgenic Nicotiana benthamiana plants encoding wild type, truncated and point mutants of the tobacco etch virus (TEV) coat protein (CP) was analyzed. After R1 plants from 45 transgenic lines were challenged with TEV, six percent of the lines exhibited high resistance, 38% exhibited low resistance, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0577-y
更新日期：2005-12-01 00:00:00
abstract：:Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) polyhedrin gene from natural mutant strain B, B2 and P was cloned and the amino acid sequence was compared with that of wild-type virus (strain H). Chimeric polyhedrin genes (8 types) containing only one site of mutation within the amino acid sequence were also constr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050283
更新日期：1998-01-01 00:00:00
abstract：:Insertion of foreign DNA containing the E. coli gpt marker by homologous recombination in the pigeonpox virus (PPV) thymidine kinase (TK) gene and selection for the presence of this DNA in the viral genome produced unstable recombinants after 3 plaque purifications. We highlight the persistence of duplicated TK DNA se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01378643
更新日期：1993-01-01 00:00:00
abstract：:In order to differentiate recent isolates of avian infectious bronchitis virus (IBV) in Taiwan, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and direct sequencing methods were used to type 25 IBV Taiwan isolates. Two conserved sequences that flank the hypervariable region I (HVR I)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718291
更新日期：1996-01-01 00:00:00
abstract：:Complete nucleotide (nt) and deduced amino acid sequences of two onion yellow dwarf virus (OYDV) isolates showing mild and severe symptoms in onion but being unable to infect garlic were determined. The genomes consisted of 10,459 and 10,461 nt (without the 3' poly(A) tail) and were 92.2 % identical. Comparison of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1597-z
更新日期：2013-06-01 00:00:00
abstract：:Since late 2018, foot-and-mouth disease (FMD) has reemerged and rapidly swept through pig farms in North and Central Vietnam, despite widespread use of commercial FMD vaccines. To investigate the FMD virus (FMDV) strains responsible for the current epidemics, 40 FMDV samples were collected from 17 provinces during Nov...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04763-8
更新日期：2020-11-01 00:00:00
abstract：:Our previous studies demonstrated that hepatitis E virus (HEV) requires the multivesicular body (MVB) pathway to release virus particles, suggesting that HEV utilizes the cellular ESCRT machinery in the cytoplasm, not at the cell surface, to be released from infected cells. In this study, we generated a murine monoclo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1912-3
更新日期：2014-05-01 00:00:00
abstract：:Human cytomegalovirus (HCMV), oxidized by sodium periodate (NaIO4), is incapable of giving rise to viral progeny in cell culture. At a NaIO4 concentration as low as 5 mM, there is a loss of at least 6 logs of viral infectivity which occurs very rapidly (less than 5 min). Further, the inactivation is a first-order reac...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309765
更新日期：1994-01-01 00:00:00
abstract：:Tunicamycin inhibited the production of infectious Borna disease virus (BDV) and glycosidase treatment eliminated the infectivity of cell-free virus. A glycoprotein of approximately 17 kDa, found in association with infectious virus, was identified by Concanavalin A binding. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309486
更新日期：1994-01-01 00:00:00
abstract：:A potyvirus isolated from Pinellia ternata in China was characterised and shown to be related to Soybean mosaic virus (SMV). The virus was pathogenic on P. ternata and some soybean cultivars, whereas the local soybean SMV isolate HH5 did not infect P. ternata. Western blot experiments demonstrated a serological relati...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0184-8
更新日期：2004-02-01 00:00:00
abstract：:A comparison of 15 field isolates of grapevine leafroll-associated virus 5 (GLRaV-5) was conducted, based on the analysis of nucleotide sequences of two viral ORFs: the coat protein (CP) and the heat shock protein 90 homolog (HSP90h). After amplification and cloning, the population of viral sequences was analyzed for ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1371-2
更新日期：2012-09-01 00:00:00
abstract：:Tetracyclines have been used to treat many bacterial infections. The use of these antibiotics for the treatment of viral diseases dates to the 1960s to 1970s. Over the decades, the effect of tetracyclines on the pathogenesis of viral infections has been demonstrated both clinically and experimentally. Tetracyclines ca...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-020-04860-8
更新日期：2021-01-01 00:00:00
abstract：:A full-length cDNA clone of olive latent virus 1 (OLV-1), a member of the genus Necrovirus, family Tombusviridae, was subjected to site-directed mutagenesis, and coat protein gene mutants were constructed. A mutant clone, denoted Delta3297, was obtained by deleting the nucleotide in position 3297, thus inducing a fram...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0767-2
更新日期：2006-10-01 00:00:00
abstract：:The full length gene for the coat protein (CP) of the potyvirus, Johnsongrass mosaic virus, was incorporated into recombinant baculovirus and expressed in insect cells. Western blot and Coomassie-stained polyacrylamide gel electrophoresis analysis of infected insect cells demonstrated that CP was produced in large qua...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01321065
更新日期：1994-01-01 00:00:00
abstract：:Senecavirus A (SVA) is associated with vesicular disease in swine and the acute death of neonatal piglets. Here, senecavirus A was isolated from a pig displaying vesicular disease in Northeast China. The virus was designated as SVA/HLJ/CHA/2016 and its full-length nucleotide sequence was determined and analyzed in com...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3480-4
更新日期：2017-10-01 00:00:00
abstract：:We have examined by immunofluorescent antibody staining technique the expression of herpes simplex virus type 1 (HSV-1) in organ cultures of the normal human oral mucosa. The expression of HSV-1 antigen was found selectively in the epithelial cell layers in relatively undifferentiated states such as basal layer and lo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310061
更新日期：1987-01-01 00:00:00
