Abstract：
:The entry modes of Japanese encephalitis (JE) and dengue-2 (DEN-2) viruses into C6/36 mosquito cells and of DEN-2 virus into human peripheral blood monocytes in vitro were studied. Inoculation of either JE or DEN-2 virions into C6/36 cells resulted in direct penetration of the virions into the cytoplasm at the cell surface in 3 stages. At stage 1, virions attached to the plasma membrane of host cells by their envelope spikes; at stage 2, the virion envelopes approximated to and eventually overlapped the host plasma membrane, and in the process the plasma membrane at the attachment sites dissolved; and, at stage 3, virions penetrated into the cytoplasm through the plasma-membrane disruptions created at the adsorption sites. Virions themselves apparently disintegrated at or near the penetration sites, for no virions were seen in the deeper cytoplasm. Coated pits did not form at the virion attachment sites, and virion-containing vesicles were not found in the cytoplasm. In the entry of DEN-2 virus into human peripheral blood monocytes, virions were found, adsorbed onto the external surface of the plasma membrane and attached to the luminal surface of macropinocytic vacuolar membranes. The latter apparently occurred as the result of ruffling and macropinocytic activities of the cells. At both sites virions penetrated into the cytoplasm through the plasma or vacuolar membrane in the same manner as they did through the plasma membrane of C6/36 cells. No evidence of viral entry by receptor-mediated endocytosis was observed. Implications of the entry mode of the mosquito cell-generated DEN-2 virus into human peripheral blood monocytes to an early process of natural, mosquito-transmitted infection is discussed.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Hase T,Summers PL,Eckels KHdoi
10.1007/BF01313814subject
Has Abstractpub_date
1989-01-01 00:00:00pages
129-43issue
1-2eissn
0304-8608issn
1432-8798journal_volume
104pub_type
杂志文章abstract：:Porcine circovirus type 2 (PCV2) is the essential infectious agent causing porcine circovirus-associated disease (PCVD) in pigs and one of the important viruses that severely jeopardize the swine husbandry industry. PCV2 elicits the unfolded protein response (UPR) via activation of the PERK pathway, and its capsid pro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04185-1
更新日期：2019-05-01 00:00:00
abstract：:The basic structure of human immunodeficiency virus type 1 (HIV-1) has been investigated morphologically; however, the internal structure of HIV-1 core is not well understood. We studied the internal structures by transmission electron microscopy. We modified the method for electron staining of ultrathin sections and ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050083
更新日期：1997-01-01 00:00:00
abstract：:An F35L mutation in the N-terminal domain of the polymerase acidic protein (PA-Nter), which contains the active site of the endonuclease, has been reported to result in higher polymerase activity in mouse-adapted strains of the 2009 pandemic influenza A H1N1 virus. We modeled wild and mutant complexes of uridine 5'-mo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3681-x
更新日期：2018-04-01 00:00:00
abstract：:We investigated the potential of Nicotiana benthamiana to express the E7 protein of human papillomavirus 8 (HPV-8), a paradigm genotype among cutaneous HPVs. The protein, modified in its putative pRb-binding domain (E7(QGD)), was transiently expressed in leaves following infiltration with agrobacteria carrying either ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0893-8
更新日期：2011-04-01 00:00:00
abstract：:A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection. The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR was performed. A common primer set for S-RNA of the Simbu serogroup of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0838-y
更新日期：2002-08-01 00:00:00
abstract：:The C-terminal R peptide of ecotropic murine leukemia virus (MLV) envelope protein (Env) negatively controls membrane fusion activity. The R peptide cleavage during virion maturation activates its fusogenicity and is required for viral entry. We analyzed fusogenicity and transduction efficiency of mutant Env proteins ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1054-6
更新日期：2007-01-01 00:00:00
abstract：:Samples containing two viruses belonging to the genus Polerovirus, beet chlorosis virus (BChV) and beet mild yellowing virus (BMYV), were collected from French and Polish sugar beet fields. The molecular properties of 24 isolates of BChV and BMYV were investigated, and their genetic diversity was examined in the coat ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2245-6
更新日期：2015-02-01 00:00:00
abstract：:Toscana Virus, a sandfly-associated Phlebovirus, was adapted to grow in cultured Aedes pseudoscutellaris (AP-61) mosquito cell line. No evidence of virus growth was seen after primary infection of cell monolayers under maintenance conditions. On the contrary, persistent infections were established by subculturing infe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317004
更新日期：1985-01-01 00:00:00
abstract：:The complete nucleotide sequence of the ssRNA genome of a Chinese GPV isolate of barley yellow dwarf virus (BYDV) was determined. It comprised 5673 nucleotides, and the deduced genome organization resembled that of members of the genus Polerovirus. It was most closely related to cereal yellow dwarf virus-RPV (77% nt i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0415-8
更新日期：2009-01-01 00:00:00
abstract：:Exposure of human lung fibroblasts to human cytomegalovirus (HCMV) stimulated a rapid increase in the release of [3H] from cells prelabelled with radiolabelled arachidonic acid ([3H]AA). Maximum stimulation of [3H] release was observed at 20 min postinfection and was quantitatively similar to that induced by the tumor...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316678
更新日期：1990-01-01 00:00:00
abstract：:Fecal virus shedding, seroconversion and histopathology were evaluated in 3-7-year-old gnotobiotic calves orally inoculated with porcine deltacoronavirus (PDCoV) (9.0-9.6 log10 genomic equivalents [GE] of OH-FD22-P5; n=4) or porcine epidemic diarrhea virus (PEDV) (10.2-12.5 log10 GE of PC21A; n=3). In PDCoV-inoculated...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3351-z
更新日期：2017-08-01 00:00:00
abstract：:The present study confirms that herpesviruses (LF) previously isolated from testes and buffy coat cells of male turkeys with semen abnormalities establish a latent infection in testicular cells. These experiments also present the first evidence that these herpesviruses are harbored latently in cells of spinal ganglia....
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311163
更新日期：1984-01-01 00:00:00
abstract：:The complete nucleotide sequence of genome segment S9, which was reported as the ninth largest among the 12 genome segments of rice dwarf virus, was determined. The segment was 1,305 bp long and coded for a protein composed of 351 amino acids (Mr 38,930). The terminal sequences of the segment were found to consist of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313885
更新日期：1989-01-01 00:00:00
abstract：:Degenerate primers were used to detect and amplify 3'-terminal genome fragments of potyviruses from medicinal aroid plants growing at 16 sites in China. Virus was detected in 7 samples of which six, all of Pinellia ternata, contained a strain of soybean mosaic virus (SMV) similar to that previously reported from this ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0390-z
更新日期：2005-01-01 00:00:00
abstract：:The RNAs of 4 avian rotavirus isolates were analysed by polyacrylamide gel electrophoresis. One of these isolates was made from chickens, the other three were from turkeys. The RNAs were separated into 8 or 9 bands consisting of 11 segments, ranging in molecular weight from 0.24 X 10(6) to 2.09 X 10(6). In contrast to...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320765
更新日期：1980-01-01 00:00:00
abstract：:Coxsackievirus B (CVB) can cause aseptic meningitis, myocarditis and respiratory disease, especially in newborn infants. To compare the susceptibility to CVB infection of fetal and adult mice, we prepared primary alveolar epithelial cells (AECs) from lungs of BALB/c mice. In contrast to fetal mouse AECs, those of adul...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1254-6
更新日期：2012-06-01 00:00:00
abstract：:Drosophila C virus RNA acted as mRNA in rabbit reticulocyte lysates and directed the synthesis of at least one capsid protein and a number of higher molecular weight proteins. Kinetic analysis by pulse-chase experiments showed that a number of high molecular weight products acted as precursors to the capsid protein(s)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311694
更新日期：1983-01-01 00:00:00
abstract：:Molecular variability was assessed for 18 isolates of cowpea mild mottle virus (CPMMV, genus Carlavirus, family Betaflexiviridae) found infecting soybean in various Brazilian states (Bahia, Goiás, Maranhão, Mato Grosso, Minas Gerais, Pará) in 2001 and 2010. A variety of symptoms was expressed in soybean cv. CD206, ran...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1879-0
更新日期：2014-04-01 00:00:00
abstract：:Non-structural as well as VP1 recombinant proteins of foot-and-mouth disease virus (FMDV) produced in E. coli, have been used to study the specific antibody response of infected or vaccinated swine. An analysis of sera from infected pigs, using a direct ELISA, showed that polypeptide 3ABC (spanning non-structural prot...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01538822
更新日期：1994-01-01 00:00:00
abstract：:Rabbit convalescent and hyperimmune sera, human patient and blood donor sera, as well as cerebrospinal fluids of humans with herpes simplex virus encephalitis all recognize similar major antigenic components in herpes simplex virus infected rabbit or human cells as shown by electrophoretic analysis of immunoprecipitat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314855
更新日期：1977-01-01 00:00:00
abstract：:Multidrug-resistant uropathogenic Escherichia coli (UPEC) is increasing gradually on a worldwide scale. We therefore examined the possibility of bacteriophage (phage) therapy for urinary tract infections (UTIs) caused by the UPEC strains as an alternative to chemotherapy. In addition to the well-known T4 phage, KEP10,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0031-4
更新日期：2008-01-01 00:00:00
abstract：:Brefeldin A (BFA) induced a rapid redistribution of vesicular stomatitis virus G protein (VSV-G) to the endoplasmatic reticulum (ER) in interferon (IFN)-pretreated cells. This result is consistent with accumulation of VSV-G in the trans-Golgi (GC) complex in cells pretreated with IFN and implies that IFN does not inte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314635
更新日期：1992-01-01 00:00:00
abstract：:Borna disease virus (BDV) polymerase can be reconstituted by co-expression of the viral genes N, P and L. N codes for two proteins, p39 and p38, resulting from alternative translation initiation. Using a viral minireplicon system we observed that unlike p39, p38 was almost completely inactive when expressed with P and...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0327-6
更新日期：2004-07-01 00:00:00
abstract：:The complete genomic sequence of Cassava Ivorian bacilliform virus (CIBV) is described. The virus has a genomic organization similar to that of pelargonium zonate spot virus (PZSV), the type member of the genus Anulavirus, but it is most closely related to a second, recently described, anulavirus, Amazon lily mild mot...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2086-3
更新日期：2014-10-01 00:00:00
abstract：:The highly conserved encapsidation signal (epsilon) of hepatitis B viral (HBV) pregenomic RNA has been reported as an essential component for encapsidation and protein priming of HBV polymerase. Here, we report that two HBV epsilon RNA-binding host proteins (80 and 43 kDa) and a copurifying protein (100 kDa) were puri...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050200001
更新日期：2002-03-01 00:00:00
abstract：:Infectious cDNA clones originally derived from a mild strain of Pepper mild mottle virus were constructed by replacing residue 649, a critical point for attenuation of this virus, with all possible amino acids. All clones were infectious to pepper plants and induced a variety of symptoms, including no visible symptoms...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0312-1
更新日期：2009-01-01 00:00:00
abstract：:Beauveria bassiana is a ubiquitous entomopathogen infecting hundreds of insect species. We have determined the genomic organization and the complete nucleotide sequence of a novel virus isolated from the isolate A24 of B. bassiana. Phylogenetic analysis of the polymerase gene reveals that the virus, tentatively named ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2416-0
更新日期：2015-06-01 00:00:00
abstract：:Mutations were introduced by recombinant DNA techniques into 9 genes of an infectious molecular clone of human immunodeficiency virus type 1. The 24 mutants generated were characterized biochemically and biologically by transfection and infection experiments. None of the mutants which have mutations in gag (p17, p24, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310491
更新日期：1991-01-01 00:00:00
abstract：:Viral infection is usually initiated by the binding of virus particles to specific receptor molecule(s) on the host cell surface. Blocking of this step prevents the following step, penetration into the cell. In the present study, we investigated the virus-cell interactions of virions of Norwalk virus (NV), a major eti...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0143-4
更新日期：2003-09-01 00:00:00
abstract：:The prevalence of HIV-1 infection in men who have sex with men (MSM) in China has drastically increased, and circulating strains may have acquired transmitted drug resistance (TDR). We determined TDR genotypes among antiretroviral therapy (ART)-naïve MSM in 19 provinces/cities where HIV-1 prevalence among MSM is high,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1557-7
更新日期：2013-04-01 00:00:00
