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Growth of the Phlebovirus Toscana in a mosquito (Aedes pseudoscutellaris) cell line (AP-61): establishment of a persistent infection.

Abstract：

:Toscana Virus, a sandfly-associated Phlebovirus, was adapted to grow in cultured Aedes pseudoscutellaris (AP-61) mosquito cell line. No evidence of virus growth was seen after primary infection of cell monolayers under maintenance conditions. On the contrary, persistent infections were established by subculturing infected cultures. Cytopathic effect was never observed. Significant titres of virus (10(3)-10(5) PFU/ml), as assayed in Vero cells at 37 degrees C, were released from persistent infected cells after several subcultures at 29 degrees C over a period of six months. The percentage of virus-producing cells in the persistently infected cultures varied from 0.003 to to 0.017 per cent, whereas from 10 to 50 per cent of cells were shown to retain viral antigens by immunofluorescence. The virus released from persistently infected cultures did not show changes in both plaque size and temperature sensitivity from the parental virus. The virus released from persistently infected cultures multiplied in AP-61 cell monolayers reaching relatively high titres (10(4) PFU/ml).

journal_name

Arch Virol

journal_title

Archives of virology

authors

Nicoletti L,Verani P

doi

10.1007/BF01317004

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

35-45

issue

1-2

eissn

0304-8608

issn

1432-8798

journal_volume

85

pub_type

杂志文章

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