Abstract：
:A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved by rate zonal centrifugation in a 7--35 per cent sucrose gradient. This procedure permits a recovery of approximately 40 per cent of viral infectivity. A specific infectivity of about 2 X 10(7) PFU/microgram of protein was achieved. An apparent density d=1.13 g/ml in sucrose and a Sw20 value of about 150 was determined for purified HC virions.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Laude Hdoi
10.1007/BF01314377subject
Has Abstractpub_date
1977-01-01 00:00:00pages
41-51issue
1-2eissn
0304-8608issn
1432-8798journal_volume
54pub_type
杂志文章abstract：:Porcine endogenous retroviruses (PERV) are widely distributed in the genomes of pigs. PERV-A and PERV-B are present in all pigs. They infect human cells in vitro and therefore represent a risk for xenotransplantation when pig cells, tissues or organs are used. PERV-C infects only pig cells and is not present in the ge...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1490-9
更新日期：2013-02-01 00:00:00
abstract：:In order to differentiate recent isolates of avian infectious bronchitis virus (IBV) in Taiwan, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and direct sequencing methods were used to type 25 IBV Taiwan isolates. Two conserved sequences that flank the hypervariable region I (HVR I)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718291
更新日期：1996-01-01 00:00:00
abstract：:A potyvirus from Chinese narcissus was transmitted mechanically to three species of Narcissus and to Lycoris radiata but not to 22 other test species. In western blot, the coat protein reacted strongly with Narcissus degeneration virus (UK isolate) antiserum. Antiserum raised to the Chinese virus did not react with ei...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0841-9
更新日期：2007-02-01 00:00:00
abstract：:Hepatitis E represents an important public-health concern throughout the world. It is one of the leading causes of hepatitis in North Africa, Asia and the Middle East. In Tunisia, the true burden of HEV infection is still unknown. The objectives of the present study were to assess the occurrence of hepatitis E virus i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2251-8
更新日期：2015-01-01 00:00:00
abstract：:A simultaneous decay of the expression of Im 140 kDa, Im 150 kDa and Im 160 kDa high MW membrane antigens, concomitant with the cell proliferation arrest, was observed during erythropoietin induced differentiation of ts 34 AEV-transformed erythroid cells cultivated at the restrictive temperature. Expression of embryo-...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310975
更新日期：1987-01-01 00:00:00
abstract：:The nucleotide sequences of the SH gene of 45 new mumps virus isolates derived from different parts of Europe, Canada and USA were determined. A phylogenetic tree was constructed which confirmed the existence of three major clusters. While clustering according to geographical origin was clear in some cases each of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050073
更新日期：1997-01-01 00:00:00
abstract：:Human bocavirus (HBoV) is a new parvovirus associated with acute respiratory tract infection (ARTI). In order to evaluate HBoV significance as an agent of acute respiratory disease, we screened 1,135 respiratory samples from children and adults with and without symptoms during two complete calendar years. HBoV1 preval...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2238-5
更新日期：2015-01-01 00:00:00
abstract：:A non-neutralizing monoclonal antibody (YO-60) against human rotavirus was found to be directed to VP2 (90,000-dalton protein), one of the two major components of the inner capsid. The reactivity patterns of the YO-60 antibody were very similar, though not identical, to those of subgroup II-specific YO-5 monoclonal an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310550
更新日期：1986-01-01 00:00:00
abstract：:Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major anti...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070087
更新日期：2000-01-01 00:00:00
abstract：:The genomes of two neurovirulent strains of poliovirus type 3, wild type P3/Leon/37 and a vaccine revertant P3/119/70, have been cloned in E. coli. The cDNA: RNA hybrid method used was efficient and may have wide applicability for cDNA cloning. Overlapping clones spanning the entire genome were obtained for each strai...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309297
更新日期：1984-01-01 00:00:00
abstract：:Autographa californica M nucleopolyhedrovirus (AcMNPV) is a large ds DNA virus restricted to larval lepidopteran insect hosts. Using field inversion gel electrophoresis and digestion with a restriction enzyme which cuts the AcMNPV genome once, we detected multiple unit-length genome fragments from replicating viral DN...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050229
更新日期：1997-01-01 00:00:00
abstract：:Dengue virus (DENV) causes a life-threatening illness, with a wide range of symptoms from mild febrile illness, dengue fever (DF), to life-threatening illness, dengue hemorrhagic fever (DHF). Antibody-dependent enhancement (ADE) is considered to be a risk factor for DHF. In the present study, we determined the paramet...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1787-3
更新日期：2014-01-01 00:00:00
abstract：:Feline calicivirus (FCV) is a highly contagious pathogen that causes oral and upper respiratory tract disease in cats. Despite widespread vaccination, the prevalence of FCV remains high. Furthermore, a high gene mutation rate has led to the emergence of variants, and some infections are lethal. To date, there is no ef...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2534-8
更新日期：2015-12-01 00:00:00
abstract：:Human bocavirus (HBoV) has been detected primarily in children with acute lower respiratory tract disease (LRTD), but its occurrence, clinical profile, and role as a causative agent of RTD are not clear. The aim of this study was to investigate the prevalence and the potential clinical relevance of HBoV. Using molecul...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04396-6
更新日期：2019-12-01 00:00:00
abstract：:Viruses represent some of the deadliest pathogens known to science. Recently they have been reported to have mutualistic interactions with their hosts, providing them direct or indirect benefits. The mutualism and symbiogenesis of such viruses with lower eukaryotic partners such as fungi, yeast, and insects have been ...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-017-3686-5
更新日期：2018-04-01 00:00:00
abstract：:Sequence analysis of a 6.4 kb DNA region from the Spodoptera littoralis multinucleocapsid nucleopolyhedrovirus (SpliNPV) revealed a large open reading frame (ORF) encoding a predicted polypeptide of 998 amino acid (aa) residues with a molecular mass of 114.93 kDa, located between 47.2-52.3 m.u. on the SpliNPV genome. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170177
更新日期：2001-01-01 00:00:00
abstract：:Human enterovirus 71 (EV71) causes hand, foot and mouth disease in children under 6 years of age, and the neurological complications of this virus can lead to death. Until now, no vaccines or drugs have been available for the clinical control of this epidemic. Macrophages can engulf pathogens and mediate a series of h...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1495-4
更新日期：2013-02-01 00:00:00
abstract：:Three ponies were inoculated with plasma containing 10(4.8) TCID50 of equine infectious anemia virus (EIAV) and observed for 165 to 440 days. Each pony developed a febrile response within 3 weeks of infection during which a plasma viremia greater than or equal to 10(3.5) TCID50/ml was observed. Analyses of four isolat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311054
更新日期：1990-01-01 00:00:00
abstract：:Long terminal repeats (LTRs) of human endogenous retroviruses (HERVs) have been reported to serve as alternative promoters in functional genes. The GSDML (gasdermin-like protein) gene located on human chromosome 17q21 has been found to be an oncogenomic recombination hotspot. Here, we identified the LTR element of HER...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0764-5
更新日期：2006-10-01 00:00:00
abstract：:Bubaline alphaherpesvirus 1 (BuHV1) is a member of the family Herpesviridae, subfamily Alphaherpesvirinae, genus Varicellovirus. To date, no full genome sequence of BuHV has been published. Here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (BuHV1) strain b6 (BuHV1-b6), isolated from a water b...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3218-8
更新日期：2017-05-01 00:00:00
abstract：:The bisegmented genome of a putative double-stranded (ds) RNA virus from Ustilaginoidea virens was sequenced and analyzed. The larger genomic segment of 2112 bp encodes a putative RNA-dependent RNA polymerase (RdRp, 628 aa), and the smaller one of 2082 bp encodes a putative coat protein (CP) of 539 aa. The 5' untransl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-1991-9
更新日期：2014-07-01 00:00:00
abstract：:The complete genome sequence of a Chinese very virulent infectious bursal disease virus (vvIBDV) strain, Harbin-1, was determined. Based on the sequence analysis, the molecular characteristics and potential virulence determinants and origin of vvIBDV strains were identified. Phylogenetic analysis indicated that a reas...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0140-8
更新日期：2008-01-01 00:00:00
abstract：:Trigeminal ganglia from swine latently infected with pseudorabies virus (PRV) were examined for the presence of PRV nucleic acids by in situ hybridization. Both viral DNA and RNA were detectable in latently infected neurons, indicating that the PRV genome is transcriptionally active during the latent phase of infectio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01321010
更新日期：1988-01-01 00:00:00
abstract：:Two independent isolates of an unusual type of rotavirus were obtained from over 900 faecal samples obtained from piglets with diarrhoea. Genome profiles, containing eleven segments, were not characteristic of group A electropherotypes and corresponded to an atypical virus. The profiles were, however, distinct from th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309892
更新日期：1986-01-01 00:00:00
abstract：:The global emergence of canine parvovirus type 2c (CPV-2c) has been well documented. In the present study, 139 rectal swab samples collected from diarrheic dogs living in Vientiane, Laos, in 2016 were tested for the presence of the canine parvovirus (CPV) VP2 gene by PCR. The results showed that 82.73% (115/139) of do...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3212-1
更新日期：2017-05-01 00:00:00
abstract：:Recently, five new viruses from the genus Vitivirus were identified and named grapevine virus G, H, I, J and L. These viruses were targeted in a survey to evaluate their prevalence in different grapevine populations in California. Excluding a single detection of GVJ, other vitiviruses were detected infecting several g...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04355-1
更新日期：2019-10-01 00:00:00
abstract：:Noroviruses cause acute viral gastroenteritis worldwide. They are classified in five genogroups, of which GI, GII, and GIV infect humans. Little information is available on the prevalence and clinical effects of GIV noroviruses. We conducted a large-scale molecular-epidemiological investigation, a yearlong monitoring ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0619-y
更新日期：2010-04-01 00:00:00
abstract：:The genome of yam virus X (YVX), a new member of the genus Potexvirus from yam (Dioscorea trifida), was completely sequenced. Structural and phylogenetic analysis showed that the closest relative of YVX is nerine virus X. A prevalence study found YVX only in plants maintained in Guadeloupe and showed that it also infe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2211-3
更新日期：2014-12-01 00:00:00
abstract：:The acid-extractable leaf proteins of potato spindle tuber viroid (PSTV) infected tomato plants were analysed electrophoretically on polyacrylamide gels. The most prominent alteration found during disease development was the appearance of a "pathogenesis-related" protein with an apparent molecular weight of 14,000 (ca...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309998
更新日期：1984-01-01 00:00:00
abstract：:Noninfectious virus particles were produced in Ehrlich ascites tumor cells infected intraperitoneally with fowl plague virus. The PFU yield of virus per cell was less than 0.1 and the ratio PFU/HA units in the progeny virus was less than 10(3). The virus particles had the same morphology and size as egg-grown virus bu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315278
更新日期：1978-01-01 00:00:00
