Isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from Jiangsu Province, China.

Abstract:

:In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 10(6.125) TCID50/ml. By applying an 80 % nucleotide cutoff value and the RotaC(2.0) automated genotyping tool, the Vp4 genotype of the new isolate was identified as P[7]. The Vp7 genotype was identified as G[9], lineage VI, and sublineage c. Experimentally infected piglets showed severe diarrhea symptoms 16-24 h post-inoculation, indicating that this new porcine rotavirus isolate is a pathogenic strain.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Zhang H,Zhang Z,Wang Y,Wang X,Xia M,Wu H

doi

10.1007/s00705-015-2347-9

subject

Has Abstract

pub_date

2015-05-01 00:00:00

pages

1333-8

issue

5

eissn

0304-8608

issn

1432-8798

journal_volume

160

pub_type

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