Abstract：
:orf124 (ac124) of AcMNPV is one of the highly conserved unique genes in group I lepidopteran nucleopolyhedroviruses. So far, its function remains unknown. In this study, infection with a virus expressing an ac124-gfp fusion showed that Ac124 localized to the cytoplasm throughout the infection. In addition, an ac124 knockout virus was generated to determine the role of ac124 in the baculovirus life cycle. Our results showed that an ac124 knockout AcMNPV could produce infectious budded viruses (BVs) and occlusion bodies (OBs) like those produced by the wild virus and ac124 repair virus. These three viruses had similar growth kinetics during the infection phase. There was no significant difference in nucleocapsids, occlusion-derived viruses and OBs visualized by electron microscopy. The ac124 deletion mutant did not reduce AcMNPV infectivity for S. exigua in an LD50 bioassay. However, it took 20 h longer for the ac124 deletion mutant to kill S. exigua than wild-type virus in the LT50 bioassay. Altogether, these results demonstrate that ac124 is not required for viral replication, but it accelerates the killing of infected larvae.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Liang C,Lan D,Zhao S,Liu L,Xue Y,Zhang Y,Wang Y,Chen Xdoi
10.1007/s00705-014-2277-ysubject
Has Abstractpub_date
2015-01-01 00:00:00pages
275-84issue
1eissn
0304-8608issn
1432-8798journal_volume
160pub_type
杂志文章abstract：:BK virus (BKV)-transformed cells and BKV-induced tumors as well as in vitro derived tumor cell lines were all found to contain BKV DNA sequences when analysed by DNA-DNA reassociation kinetics. In transformed cells the number of viral genome equivalents per diploid cell genome (CG divided by VG) decreased with increas...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314838
更新日期：1981-01-01 00:00:00
abstract：:Integration of bovine leukaemia virus DNA was investigated at all stages of infection in cattle. We report here the detection of integrated proviral DNA in the majority of antibody positive animals without lymphocytosis. In all but one case virus was integrated at a number of different sites. Hybridization analysis fa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314319
更新日期：1991-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3618-4
更新日期：2018-02-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315054
更新日期：1977-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310678
更新日期：1991-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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journal_title：Archives of virology
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doi：10.1007/s00705-013-1873-6
更新日期：2014-04-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050439
更新日期：1998-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04726-z
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更新日期：2015-08-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2042-2
更新日期：2014-08-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04182-4
更新日期：2019-04-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0735-x
更新日期：2006-08-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04251-8
更新日期：2019-07-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309765
更新日期：1994-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1315-x
更新日期：2012-07-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070013
更新日期：2000-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0330-y
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pub_type： 杂志文章
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316907
更新日期：1993-01-01 00:00:00
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更新日期：2013-10-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314433
更新日期：1987-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：2016-11-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313899
更新日期：1987-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718222
更新日期：1996-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s705-002-8310-6
更新日期：2002-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-03-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1280-4
更新日期：2012-06-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3534-7
更新日期：2017-12-01 00:00:00
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journal_title：Archives of virology
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doi：10.1007/s00705-016-2955-z
更新日期：2016-09-01 00:00:00
