Abstract：
:Three sets of primers to detect foot-and-mouth disease virus (FMDV) using multiplex RT-PCR were designed based on several reference nucleotide sequences, and their reaction conditions were determined. By testing ten-fold serial dilutions of FMDV, the sensitivity of multiplex RT-PCR is 100 times higher than conventional RT-PCR. Meanwhile, its specificity was confirmed compared with other related vesicular disease viruses. Furthermore, 30 field samples from different animals were tested, and the results supported the method's potential applications in routine veterinary quarantine and epidemic surveillance of FMDV.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Bao HF,Li D,Guo JH,Lu ZJ,Chen YL,Liu ZX,Liu XT,Xie QGdoi
10.1007/s00705-007-1082-ysubject
Has Abstractpub_date
2008-01-01 00:00:00pages
205-9issue
1eissn
0304-8608issn
1432-8798journal_volume
153pub_type
杂志文章abstract：BACKGROUND:In May 2018, a 8 year old thoroughbred mare died at an equestrian club in Changji, Xinjiang, China. The horse had been imported from the United States in 2013. She became pregnant in December 2016 but, after foaling, gradually lost weight and died in May 2018. This study aim to identify the pathogen, who cau...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04487-4
更新日期：2020-02-01 00:00:00
abstract：:Mengovirus, extracted from infected L-cell cultures with Freon 113 and concentrated from the aqueous phase with polyethyleneglycol, was chromatographed on protein-coated controlled pore glass (CPG). The covalent binding of protein to CPG is described. Further purification and concentration of mengovirus was achieved b...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315283
更新日期：1978-01-01 00:00:00
abstract：:The alteration of whole Sendai virus and especially of its nucleocapsid polypeptides, during storage of the virus at 4 degree C in the allantoic fluids in which it was cultivated, has cultivated, has been studied by sodium dodecyl sulfate gel electrophoresis. During virus storage the nucleocapsid protein subunits with...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317427
更新日期：1975-01-01 00:00:00
abstract：:In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of seque...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1141-6
更新日期：2012-01-01 00:00:00
abstract：:To trace the possible route of introduction of porcine epidemic diarrhea virus (PEDV), the phylogenetic relationships of PEDV strains in the regions of epidemicity in the Philippines to PEDV strains that are endemic in other countries were investigated. Partial nucleotide sequences of the S1 spike gene was determined ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2938-0
更新日期：2016-09-01 00:00:00
abstract：:HSV-2 infections suppress the antibody response to HSV-1 but do not impair the mouse cytomegalo virus (MCMV) antibody generating system. In contrast, the sheep red blood cell (SRBC) IgM response is impaired by preinfections with HSV-2 but not with HSV-1. From the time kinetics of this suppression it can be concluded t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310741
更新日期：1987-01-01 00:00:00
abstract：:The E5 protein of papillomaviruses is a strongly hydrophobic membrane protein that can associate with the 16 kDa protein subunit of the vacuolar proton ATPase in endosomes and the Golgi apparatus resulting in raise of intraorganelle pH. We demonstrate that E5 of human papillomavirus type 16 (HPV16) when transfected in...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070048
更新日期：2000-01-01 00:00:00
abstract：:Samples containing two viruses belonging to the genus Polerovirus, beet chlorosis virus (BChV) and beet mild yellowing virus (BMYV), were collected from French and Polish sugar beet fields. The molecular properties of 24 isolates of BChV and BMYV were investigated, and their genetic diversity was examined in the coat ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2245-6
更新日期：2015-02-01 00:00:00
abstract：:We investigated the in situ localization of the 50 kDa protein encoded by ORF2 of Apple chlorotic leaf spot virus (ACLSV) genome which is thought to be a movement protein. In immunogold electron microscopy of ACLSV-infected Chenopodium quinoa leaves, the 50 kDa protein was localized on plasmodesmata and nearby cytopla...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050660
更新日期：1999-01-01 00:00:00
abstract：:Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The 95% detection limit of the RT-RPA was 3.767 log10 genomic copies (LGC)/reacti...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3859-x
更新日期：2018-09-01 00:00:00
abstract：:Bovine leukemia virus (BLV), the etiologic agent of enzootic bovine leucosis, has caused pandemic outbreaks worldwide. Because transcription of the BLV is quickly blocked after infection, detecting integrated provirus at host genome is an important method of identifying whether an animal is infected. The aim of the pr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2806-y
更新日期：2016-06-01 00:00:00
abstract：:Gp 85 the major envelope glycoprotein of Rous sarcoma virus can be released from intact by virus treatment with 2-mercaptoethanol. Attempts were made to reassociate released gp 85 to 2-mercaptoethanol treated virus. In order to study the biological activity and to analyse the reaction product with biochemical methods ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314883
更新日期：1982-01-01 00:00:00
abstract：:A total of 72 Borna Disease virus infected rabbits were treated with different concentrations of cyclophosphamide, glucocorticoids or both in combination. Comparison with untreated, infected rabbits showed a drastic alteration in the clinical picture, a considerable prolongation of the survival time, and differences i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318132
更新日期：1981-01-01 00:00:00
abstract：:A reverse transcription-polymerase chain reaction (RT-PCR) procedure for the detection of avian, duck, and goose reovirus (ARV, DRV, and GRV) RNA from cell culture supernatant and clinical samples was established. Based on multiple sequence alignment, a pair of degenerate primers was selected and synthesized. The ampl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0731-1
更新日期：2006-08-01 00:00:00
abstract：:MicroRNAs (miRNAs) are a new class of well-conserved small noncoding RNAs that mediate posttranscriptional gene regulation. Hepatitis B virus (HBV) causes various liver diseases, including chronic hepatitis, liver cirrhosis and hepatocellular cancer. Recent data have indicated HBV alters miRNAs expression patterns, bu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2286-x
更新日期：2015-03-01 00:00:00
abstract：:When 66 cucurbit samples with yellowing symptoms from fields in Mali, the Philippines, Thailand and Uzbekistan were screened by RT-PCR using universal polerovirus primers, 21 were identified as harboring polerovirus RNA. When these 21 samples were screened with specific primers for the known cucurbit-infecting polerov...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1939-5
更新日期：2014-06-01 00:00:00
abstract：:A filamentous virus, with particles 600-650 nm long, was purified from Narcissus pseudonarcissus (daffodil) in Hangzhou and an antiserum prepared. After mechanical inoculation, the virus could be detected serologically in Narcissus species but not in some commonly used virus indicators. Infection was symptomless. The ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0801-4
更新日期：2006-11-01 00:00:00
abstract：:In this study, we isolated a novel avian reovirus (ARV) strain, K738/14, from a broiler chicken with viral arthritis in South Korea. Genome sequence comparisons showed relatively low nucleotide identity with previously identified ARV strains. Phylogenetic analyses suggested multiple reassortment events between reoviru...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3667-8
更新日期：2018-05-01 00:00:00
abstract：:The global emergence of canine parvovirus type 2c (CPV-2c) has been well documented. In the present study, 139 rectal swab samples collected from diarrheic dogs living in Vientiane, Laos, in 2016 were tested for the presence of the canine parvovirus (CPV) VP2 gene by PCR. The results showed that 82.73% (115/139) of do...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3212-1
更新日期：2017-05-01 00:00:00
abstract：:The chemical nature of the binding sites for vesicular stomatitis virus (VSV) was studied by measuring the ability of solubilized rat brain cell membranes (SRBM) to compete with cultured cells for viral binding. SRBM significantly reduced both binding and infectivity of VSV. After separation of protein and lipid compo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311075
更新日期：1988-01-01 00:00:00
abstract：:The 87,000-dalton glycoprotein (gp87) of bovine herpesvirus type 1 (BHV-1) was found to selectively attach to susceptible cells. The attachment of gp87 to the cells was markedly decreased by the prior adsorption of intact virions. Anti-gp87 (site Ia) monoclonal antibody, which inhibited BHV-1 adsorption to the cells a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314428
更新日期：1987-01-01 00:00:00
abstract：:Here, we describe for the first time the complete genome sequence of a new bipartite begomovirus in Madagascar isolated from the weed Asystasia gangetica (Acanthaceae), for which we propose the tentative name asystasia mosaic Madagascar virus (AMMGV). DNA-A and -B nucleotide sequences of AMMGV were only distantly rela...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2413-3
更新日期：2015-06-01 00:00:00
abstract：:The present study reports the first complete genome sequence analysis of West Nile virus (WNV) strains isolated from brain samples from raptors. The results prove the circulation of closely related WNV lineage II strains in central Europe and genetic analysis revealed seven amino acid substitutions in structural (PrM3...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4056-7
更新日期：2019-01-01 00:00:00
abstract：:In each infected patient, the population of hepatitis C virus is composed of quasispecies that differ in their nucleotide sequences. Among regions in hepatitis C virus genomes, nucleotide sequences of the hypervariable region have been shown to change quickly during the course of infection. It is not known, however, w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315417
更新日期：1995-01-01 00:00:00
abstract：:The genomic RNA sequences of two genotypes of a brassica-infecting polerovirus from China were determined. Sequence analysis revealed that the virus was closely related to but significantly different from turnip yellows virus (TuYV). This virus and other poleroviruses, including TuYV, had less than 90% amino acid sequ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1091-z
更新日期：2011-12-01 00:00:00
abstract：:Six isolates of Suid herpesvirus 1 (SHV-1) collected from clinical outbreaks of Aujeszky's disease (AD) in Jutland in January-February, 1985, were compared by restriction fragment pattern (RFP) analyses with older SHV-1 isolates from Jutland as well as with SHV-1 isolates from different parts of West Germany and Irela...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310912
更新日期：1988-01-01 00:00:00
abstract：:Poultry production is an important sector of agriculture in Sri Lanka; however, there is a lack of information regarding circulation of infectious bronchitis virus (IBV). RNA was extracted from chicken tissues, subjected to IBV S1 RT-PCR, and sequenced. Overall, 19 out of 34 (55.88 %) samples were IBV positive and con...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2831-x
更新日期：2016-06-01 00:00:00
abstract：:Two cases of coinfection with bovine papular stomatitis virus (BPSV) and pseudocowpox virus (PCPV) in dairy calves in Tochigi Prefecture, Japan, are reported. Sequences of BPSV and PCPV were simultaneously detected in the same polymerase chain reaction (PCR) amplicons, which were obtained from the DNA of two dairy cal...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04792-3
更新日期：2020-11-01 00:00:00
abstract：:It still needs to be elucidated whether co-detection of EV71 with other intestinal tract viruses plays a role in the pathogenesis of severe hand-foot-mouth disease (HFMD). A total of 680 stool specimens collected from clinically diagnosed mild and severe-HFMD patients were tested for EV71, CA16, norovirus, bocavirus a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1396-6
更新日期：2012-11-01 00:00:00
abstract：:Broadly neutralizing antibodies (bNAbs) are very promising agents for HIV-1 prophylaxis and AIDS treatment. However, the neutralization susceptibility of circulating recombinants such as CRF01_AE, which is becoming increasingly prevalent, has not been studied in detail until now. Here, we focused on CRF01_AE in China ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4011-7
更新日期：2018-12-01 00:00:00
