Abstract：
:In each infected patient, the population of hepatitis C virus is composed of quasispecies that differ in their nucleotide sequences. Among regions in hepatitis C virus genomes, nucleotide sequences of the hypervariable region have been shown to change quickly during the course of infection. It is not known, however, whether these variations exist in the core region that has recently been suggested to contain human lymphocyte antigen class 1 restricted sites for cytotoxic T cell recognition. To clarify this, RNA was extracted from the plasma of four patients with chronic hepatitis C. After cDNA synthesis, DNA fragments that contain the core region were amplified by the polymerase chain reaction and the diversity of the core region was analyzed by the single strand conformation polymorphism analysis. Using this method, single or multiple DNA bands were observed in each patient, and representative bands showed different nucleotide sequences. Comparison of single strand conformation polymorphism patterns revealed that the population of quasispecies changed during the course of chronic infection. These changes were more remarkable in patients with high serum alanine aminotransferase levels than those with low serum alanine aminotransferase levels. Thus, sequential variations exist in the core region of hepatitis C virus in same individuals, and the population of quasispecies as determined by the sequence of the core region changes during the course of infection, which might be related to cytopathic effects of hepatitis C virus.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Kurosaki M,Enomoto N,Marumo F,Sato Cdoi
10.1007/BF01315417subject
Has Abstractpub_date
1995-01-01 00:00:00pages
1087-94issue
6eissn
0304-8608issn
1432-8798journal_volume
140pub_type
杂志文章abstract：:Human peripheral blood mononuclear cells (PBMC) can be infected in vitro with sera of hepatitis C virus (HCV)-infected patients. We have treated pools of PBMC with dextran sulfate MW 8,000, before infection with various HCV(+) sera. When the cells were treated with dextran sulfate 10-3 M, HCV RNA was no longer detecta...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050293
更新日期：1998-01-01 00:00:00
abstract：:Banna virus, whose genome is composed of 12 segments of double-stranded RNA, is a member of the genus Seadornavirus in the family Reoviridae and is thought to be an emerging mosquito-transmitted human pathogen in Southeast Asia. A novel phylogenetic relative of Banna virus (Balaton virus, BALV, JX947843-JX947850 and K...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1712-9
更新日期：2013-10-01 00:00:00
abstract：:A full length cDNA copy of the genomic RNA of lettuce mosaic virus (LMV) was constructed under the control of an enhanced CaMV 35S promoter and of the NOS terminator. This construct was found infectious when inoculated to lettuce plants. The intron II of the bean nitrite reductase gene was engineered into the LMV FL c...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050474
更新日期：1998-01-01 00:00:00
abstract：:Many distinct polyomaviruses infecting a variety of vertebrate hosts have recently been discovered, and their complete genome sequence could often be determined. To accommodate this fast-growing diversity, the International Committee on Taxonomy of Viruses (ICTV) Polyomaviridae Study Group designed a host- and sequenc...
journal_title：Archives of virology
pub_type： 新闻
doi：10.1007/s00705-016-2794-y
更新日期：2016-06-01 00:00:00
abstract：:Amino acids Lys34, His36, and Phe37 were substituted by PCR-mediated, site-directed mutagenesis for three Trp's in the AcNPV polyhedrin sequence. Phase contrast microscopy revealed refringent, amorphous polyhedra in the nuclei of infected cells. Electron microscopy confirmed a great variation in form and size of the m...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050675
更新日期：2000-01-01 00:00:00
abstract：:The complete genome sequence of a severe isolate of broad bean true mosaic virus (genus Comovirus, subfamily Comovirinae, family Secoviridae) is presented. Comparison of the amino acid sequences of the capsid proteins and the polymerase showed striking differences to other comoviruses and highest similarities to legum...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0668-2
更新日期：2010-07-01 00:00:00
abstract：:The RNA binding properties of the prunus necrotic ringspot virus (PNRSV) coat protein (CP) were demonstrated by northwestern and dot-blot analyses. The capability to bind PNRSV RNA 4 was compared with viruses representing three different interactions prevailing in the assembly and architecture of virions. The results ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050545
更新日期：1999-01-01 00:00:00
abstract：:The full length gene for the coat protein (CP) of the potyvirus, Johnsongrass mosaic virus, was incorporated into recombinant baculovirus and expressed in insect cells. Western blot and Coomassie-stained polyacrylamide gel electrophoresis analysis of infected insect cells demonstrated that CP was produced in large qua...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01321065
更新日期：1994-01-01 00:00:00
abstract：:The distribution of African swine fever virus (ASFV) in whole blood, plasma, red blood cells (RBC) and white blood cell (WbC) sub-populations was determined in pigs infected with virulent virus. Changes in the RBC and WBC populations were also examined. Total WBC counts decreased and RBC numbers remained unchanged dur...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315054
更新日期：1977-01-01 00:00:00
abstract：:To determine the prevalence of enteroviruses in aquatic environments in Shenzhen, water samples were collected monthly from April 2010 to December 2011. After concentration by the filter adsorption-beef extract elution method, the enteroviruses were propagated in RD cells. Thirty-five of 105 water samples (33.33 %) we...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1611-0
更新日期：2013-06-01 00:00:00
abstract：:Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) polyhedrin gene from natural mutant strain B, B2 and P was cloned and the amino acid sequence was compared with that of wild-type virus (strain H). Chimeric polyhedrin genes (8 types) containing only one site of mutation within the amino acid sequence were also constr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050283
更新日期：1998-01-01 00:00:00
abstract：:A total of 472 samples from domestic pigs collected in China from 2015 to 2018 were tested for the presence of porcine circovirus types 2 and 3 (PCV2 and PCV3, respectively) by conventional polymerase chain reaction analysis. The prevalence of PCV2, PCV3, and PCV2/3 co-infection was 50.0%, 13.3%, and 6.78%, respective...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04336-4
更新日期：2019-10-01 00:00:00
abstract：:Meningitis is a serious condition that affects the central nervous system. It is an inflammation of the meninges, which is the membrane that surrounds both the brain and the spinal cord. Meningitis can be caused by bacterial, viral, or fungal infections. Many viruses, such as enteroviruses, herpesviruses, and influenz...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-020-04891-1
更新日期：2021-01-03 00:00:00
abstract：:A distinct strain of hollyhock yellow vein mosaic virus (HoYVMV) and Ludwigia leaf distortion betasatellite (LuLDB) were associated with yellow vein mosaic of hollyhock. The viral DNA genome (JQ911766) and betasatellite (JQ408216) shared highest nucleotide sequence identity (89.2 %) with HoYVMV (the only available seq...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2108-1
更新日期：2014-10-01 00:00:00
abstract：:Sequence analysis of a 6.4 kb DNA region from the Spodoptera littoralis multinucleocapsid nucleopolyhedrovirus (SpliNPV) revealed a large open reading frame (ORF) encoding a predicted polypeptide of 998 amino acid (aa) residues with a molecular mass of 114.93 kDa, located between 47.2-52.3 m.u. on the SpliNPV genome. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170177
更新日期：2001-01-01 00:00:00
abstract：:Human adenovirus Ad36 is causally and correlatively associated in animals and humans, respectively, with increased adiposity and altered metabolic profile. We inoculated rats with Ad36 or UV-inactivated Ad36, or mock-infected them. Four days later, Ad36-infected rats showed 23% greater epididymal fat pad weight and vi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0219-2
更新日期：2008-01-01 00:00:00
abstract：:Lymphocytic chorimeningitis virus (LCMV), the prototype arenavirus, and Lassa virus (LASV), causative agent of Lassa hemorrhagic fever (LHF), belong to the Old World group of the family Arenaviridae. Both viruses have extensive strain diversity and significant variations in lethality and pathogenicity for man and expe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0385-9
更新日期：2004-12-01 00:00:00
abstract：:A type III Trichomonas vaginalis virus, which may be involved in transcriptional regulation of the major surface protein gene P270 of the protozoan pathogen Trichomonas vaginalis, was purified and characterized in the present study. The complete 4844-base-pair complementary DNA sequence of the viral genome reveals ove...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0858-y
更新日期：2011-02-01 00:00:00
abstract：:Two contigs with high similarity to partitivirus sequences were identified by de novo assembly of sequences obtained by RNA-Seq from a wild brassicaceous plant, Arabidopsis halleri subsp. gemmifera. Here, we report the complete genome sequence of a putative novel partitivirus. Excluding the poly-A tail, it consists of...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04670-y
更新日期：2020-09-01 00:00:00
abstract：:The sequence of prune dwarf ilarvirus (PDV) RNA-1 has been determined; it consists of 3,374 nucleotides and contains a single open reading frame of 3,168 nucleotides. The putative translation product is 1,055 amino acids in length with a calculated molecular mass of 118.9 kDa. Both the nucleic acid and the translated ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050210
更新日期：1997-01-01 00:00:00
abstract：:Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). Although SFTS originated in China, it is an emerging infectious disease with prevalence confirmed in Japan, Korea, and Vietnam. The full-length genomes of 51 Korean SFTSV isolates from 2013 to 2016 were sequenced, and the sequences wer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04733-0
更新日期：2020-11-01 00:00:00
abstract：:Out of 136 new phages, 80 (59%) are classified into 23 species according to morphology and physicochemical properties. Six new species are described and species beta 4, from a previous classification scheme, is renamed T1. The morphology of 36 phage species is schematically represented. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Bovine herpesvirus 1 (BHV-1) is a major pathogen of cattle, causing significant disease including immunosuppression in infected animals. In vitro, the surface expression of major histocompatibility complex (MHC) class I molecules, crucial for an appropriate anti-viral immune response of the host, is down-regulated by ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070038
更新日期：2000-01-01 00:00:00
abstract：:Twenty TGEV isolates were compared by sequencing a 393-414 nucleotide stretch near the 5' end of the S gene, after amplification by RT-PCR. This part of the S gene is known to show considerable variation between porcine, canine and feline coronaviruses and is completely deleted from porcine respiratory coronaviruses. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050191
更新日期：1997-01-01 00:00:00
abstract：:Antibody and cell-mediated immune responses were measured in rabbits immunized with live, UV inactivated herpes simplex virus or with a subunit vaccine containing envelope proteins. All the types of immunization procedures induced the production of antibody as well as a specific cellular immunity. Furthermore, the sub...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317862
更新日期：1976-01-01 00:00:00
abstract：:Purified DNA from 45 isolates of suis herpes virus 1 (SHV1) collected between 1980 and 1987 from clinical outbreaks of Aujeszky's disease on French farms was compared by restriction fragment pattern (RFP) analysis. The BamHI generated RFPs were found to be distinguishable, confirming RFP analysis as a potential epidem...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01323161
更新日期：1990-01-01 00:00:00
abstract：:To evaluate the variation of Potato mop-top pomovirus from potato fields, 21 isolates were collected from different Danish locations. Reverse transcription-polymerase chain reaction-restriction fragment length polymorphism (RT-PCR-RFLP) of regions of RNA2 was performed for all 21 isolates resulting in the establishmen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0926-z
更新日期：2003-02-01 00:00:00
abstract：:Since the first rescue of a recombinant Newcastle disease virus (rNDV) in the late 1990s, many more rNDVs have been rescued by researchers around the world. Regardless of methodology, the main principle behind rescue of the virus has remained the same, i.e., the formation of a functional replication complex by simulta...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-016-3065-7
更新日期：2017-01-01 00:00:00
abstract：:Molecular variability was assessed for 18 isolates of cowpea mild mottle virus (CPMMV, genus Carlavirus, family Betaflexiviridae) found infecting soybean in various Brazilian states (Bahia, Goiás, Maranhão, Mato Grosso, Minas Gerais, Pará) in 2001 and 2010. A variety of symptoms was expressed in soybean cv. CD206, ran...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1879-0
更新日期：2014-04-01 00:00:00
abstract：:Five avian paramyxoviruses of serotype 8 (APMV-8) were isolated during a study monitoring wild birds in Kazakhstan in 2013 and each was further characterized. The viruses were isolated from three White-fronted geese (Anser albifrons), one Whooper swan (Cygnus cygnus), and one Little stint (Calidris minuta). Before our...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3593-9
更新日期：2018-02-01 00:00:00
