Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome.

Abstract:

:Herpes simplex virus type 1 (HSV-1) is a ubiquitous human pathogen of skin and mucous membranes. In the present study, the genome of the HSV-1 F strain was cloned as an infectious bacterial artificial chromosome (BAC) clone without any deletions of the viral genes. Additionally, a firefly luciferase cassette was inserted to generate a novel luciferase-expressing HSV-1 BAC. Importantly, the resulting recombinant HSV-1 BAC Luc behaved indistinguishably from the wild-type virus in Vero cells, and the luciferase activity could be easily quantified in vitro. Thus, this novel HSV-1 BAC system would serve as a powerful tool for gene function profiling.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Li Y,Wang S,Zhu H,Zheng C

doi

10.1007/s00705-011-1094-9

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

2267-72

issue

12

eissn

0304-8608

issn

1432-8798

journal_volume

156

pub_type

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