Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples.

Abstract:

:RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA species were identified. In three cases, 2-3 distinct isolates of a virus species co-infected the same plant. Twelve of the viruses identified were described previously and belonged to the genera Potyvirus, Nepovirus, Allexivirus, and Carlavirus. Four were unknown and are proposed as members of the genera Potyvirus, Sadwavirus, and Trichovirus. Virus sequences were subsequently matched to original host plants using RT-PCR assays.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Wylie SJ,Luo H,Li H,Jones MG

doi

10.1007/s00705-011-1166-x

subject

Has Abstract

pub_date

2012-02-01 00:00:00

pages

271-84

issue

2

eissn

0304-8608

issn

1432-8798

journal_volume

157

pub_type

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