Abstract:
:The acid-extractable leaf proteins of potato spindle tuber viroid (PSTV) infected tomato plants were analysed electrophoretically on polyacrylamide gels. The most prominent alteration found during disease development was the appearance of a "pathogenesis-related" protein with an apparent molecular weight of 14,000 (called P14) which is drastically increased in concentration. Its induction, however, is not viroid-specific because it is also accumulating after viral and fungal infections. The degree of P14 accumulation could be directly correlated with the severity of the disease symptoms and its concentration was found to be highest in leaves of the tomato cultivar "Rutgers" four weeks after infection. P14 was isolated from such leaf material by acid-extraction of the leaf proteins, which were concentrated from the clarified homogenates by ultrafiltration through hollow fiber systems or by precipitation at 60 per cent ammonium sulphate saturation. P14 was finally purified by ion exchange chromatography on sulfopropyl (SP-C25) Sephadex and on DEAE cellulose. A protein with properties similar to those of P14 could also be isolated from healthy tomato leaves, where its concentration is about forty to fifty times lower than PSTV-infected tissue. P14 can be stained with Coomassie Brilliant Blue, silver and ethidium bromide, it is sensitive to digestion with pronase and not altered when treated with RNase and DNase. P14 is a basic protein with an estimated isoelectric point of 10.7 and its unusual behaviour during ultrafiltration indicates that it represents an elongated rather than a globular molecule in solution. P14 seems to be different from any of the so-called "pathogenesis-related" proteins described so far in Gynura aurantiaca, "Etrog" citron, potato and tomato after viroid-infection and in tobacco, cucumber and bean leaves after virus- or fungus-induced hypersensitive reactions.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Camacho Henriquez A,Sänger HLdoi
10.1007/BF01309998subject
Has Abstractpub_date
1984-01-01 00:00:00pages
263-84issue
3-4eissn
0304-8608issn
1432-8798journal_volume
81pub_type
杂志文章abstract::We determined the complete nucleotide sequence of EDRD-1, a Japanese strain of the North American type-Porcine reproductive and respiratory syndrome virus (PRRSV), and identified a novel 117-base deletion and 108-base insertion previously reported in the nsp2 gene of the SP strain, which contains the largest genome am...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0098-6
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abstract::An archival brain sample from an infected dog taken during the 1988/89 rabies outbreak in Finland was investigated to confirm that it was infected with classical rabies virus (genotype 1). The first 400 base pairs of the virus nucleoprotein gene were then sequenced and used to undertake a phylogenetic comparison betwe...
journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
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更新日期:1981-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0309-8
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journal_title:Archives of virology
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310664
更新日期:1984-01-01 00:00:00
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更新日期:2014-12-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
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更新日期:2014-04-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01348017
更新日期:1976-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00705-013-1703-x
更新日期:2013-10-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050272
更新日期:1998-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0227-1
更新日期:2004-03-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2829-4
更新日期:2016-06-01 00:00:00
abstract::China experienced an outbreak of equine influenza during 2007-2008. Meanwhile, its neighbor countries, such as Mongolia, India and Japan, have also been affected by various influenza virus strains in each country. Phylogenetic analysis showed that the newly emerging Chinese strains belong to Florida sublineage clade 2...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0724-y
更新日期:2010-09-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310506
更新日期:1990-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
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更新日期:1980-01-01 00:00:00
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journal_title:Archives of virology
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journal_title:Archives of virology
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170193
更新日期:2001-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318132
更新日期:1981-01-01 00:00:00
abstract::RNA 3 of alfalfa mosaic virus (AMV) encodes the 5'-proximal movement protein (MP) gene and the 3'-proximal coat protein (CP) gene which is expressed from a subgenomic RNA. Several strategies were explored to use this RNA as a vector for expression of the green fluorescent protein (GFP) in Nicotiana tabaccum plants exp...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170125
更新日期:2001-01-01 00:00:00
abstract::Phages, viruses targeting bacteria, have potential therapeutic applications in the control of infections with antibiotic-resistant bacteria. In this study, an Enterobacter cloacae phage, Ec_L1, was isolated from sewage sludge samples collected from a hospital. The genome of phage Ec_L1 consists of 51,894 bp with 48.24...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04672-w
更新日期:2020-08-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311037
更新日期:1989-01-01 00:00:00
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journal_title:Archives of virology
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更新日期:2001-08-01 00:00:00
abstract::Infectious cDNA clones of the Shanghai isolate of Ribgrass mosaic virus (RMV) were produced by joining four overlapping cDNA fragments and also in a single step by long template PCR. After inoculation of Nicotiana glutinosa with either RNA transcripts or the cDNA under the control of the CaMV 35S promoter, plants deve...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0740-0
更新日期:2006-09-01 00:00:00
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journal_title:Archives of virology
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doi:10.1007/s00705-016-3205-0
更新日期:2017-04-01 00:00:00
abstract::A physical map for the genome of Spodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289-295] was prepared using the restriction endonucleases SmaI, SfiI, NotI, ApaI, KpnI, BstEII, PstI, SacI, and HindIII. The size of the geno...
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pub_type: 杂志文章
doi:10.1007/BF01313815
更新日期:1989-01-01 00:00:00