Abstract：
:vp1, a gene encoding one of the capsid proteins of Taura syndrome virus, was cloned into the pGEX-6P-1 expression vector, and the resulting construct was then used to transform E. coli strain BL21. After induction, an N-terminally glutathione-S-transferase-tagged VP1 (GST-VP1) protein with a molecular mass of 80 kDa was obtained. This protein was purified by SDS-PAGE and used for immunization of Swiss mice for monoclonal antibody (MAb) production. Three MAbs specific for the VP1 protein were selected that were suitable for detecting natural TSV infection in Penaeus vannamei by dot blotting, western blotting and immunohistochemistry. This detection occurs without cross-reaction to other shrimp tissues or other common shrimp viruses. As determined by dot blotting, the detection sensitivity of the MAbs was approximately 2 fmole/spot of the GST-VP1. These MAbs showed detection sensitivity comparable to that of MAbs specific for VP2, but they exhibited stronger immunoreactivity than previously studied MAbs specific for VP3. Although the sensitivity of the MAbs to VP1 was 1,000 times lower than one-step RT-PCR, they could be used in various types of antibody-based assays to confirm and enhance the detection sensitivity of TSV infection in shrimp.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Hajimasalaeh W,Longyant S,Chaivisuthangkura P,Sithigorngul Pdoi
10.1007/s00705-012-1460-2subject
Has Abstractpub_date
2013-01-01 00:00:00pages
77-85issue
1eissn
0304-8608issn
1432-8798journal_volume
158pub_type
杂志文章abstract：:As the most common NK/T-cell lymphoma in Asian countries, extranodal NK/T-cell lymphoma, nasal type (ENKTL), has unique clinical features and a strong association with Epstein-Barr virus (EBV). In order to gain a preliminary understanding of the relationship between ENKTL and EBV, we performed genotypic analysis of EB...
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pub_type： 杂志文章
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journal_title：Archives of virology
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1007/s007050200001
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pub_type： 杂志文章
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doi：10.1007/s00705-013-1670-2
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doi：10.1007/BF01314129
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pub_type： 杂志文章
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doi：10.1007/s007050050650
更新日期：1999-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00705-011-1085-x
更新日期：2011-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：1985-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF01311163
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pub_type： 杂志文章
doi：10.1007/BF01315089
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：1997-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3993-5
更新日期：2018-12-01 00:00:00
