Abstract：
:In this study, an improved polymerase chain reaction (PCR) was used for detection of DNA of latent EHV-1 strains from several sources. Three pairs of oligonucleotide primers spanning fragments of 333 bp, 226 bp and 268 bp of the thymidine kinase (tk) gene, and one primer pair spanning 225 bp of the glycoprotein C (gC) gene were used in specific amplifications. Primers for EHV-4 PCR were also designed. Restriction digests with TaqI confirmed the identity of tk PCR fragments from EHV-1. The sensitivity to detect PCR products was further improved by visualisation in silver-stained acrylamide gels. PCR assays were applied to 267 samples including pools of tissue, peripheral blood leukocytes (PBL) and nasal swabs of archived, farms and abattoir specimens from a total of 116 animals. The EHV-1 DNA was found in 88% of the analysed samples. The prevalence of the EHV-1 latent or persistent form in adult horses was similar to others reports but found higher than previously described in foetuses and young foals. EHV-4 latency was not detected in the Brazilian studied specimens.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Carvalho R,Oliveira AM,Souza AM,Passos LM,Martins ASdoi
10.1007/s007050070055keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
1773-87issue
9eissn
0304-8608issn
1432-8798journal_volume
145pub_type
杂志文章abstract：:In February 2008, five patients were infected with the H1N1 subtype of influenza A virus in one hospital ward for immunocompromised patients at a hospital in North Rhine-Westphalia, Germany. All of these patients had an established haematologic disease and tested positive either for viral RNA or antigen shortly after ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0771-4
更新日期：2010-11-01 00:00:00
abstract：:Human papillomavirus (HPV) type 13 DNA was detected in focal epithelial hyperplasia lesions of the oral mucosa in seven half-caste mexicans. The lesions contained intracellular papillomavirus-like particles with a diameter of about 50 nm. DNA extracted from biopsies contained unintegrated HPV type 13 DNA genomes as re...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313899
更新日期：1987-01-01 00:00:00
abstract：:Bohle iridovirus (BIV) belongs to the genus Ranavirus, of which Frog virus 3 (FV-3) is the type species. We are developing BIV as a recombinant viral delivery vector, and as a first step we located specific BIV promoter sequences to drive foreign gene expression in the recombinant virus. By comparison with FV-3 sequen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0532-y
更新日期：2005-09-01 00:00:00
abstract：:To eradicate rabies in foxes, almost 97 million oral rabies vaccine baits have been distributed in Germany and Austria since 1983 and 1986, respectively. Since 2007, no terrestrial cases have been reported in either country. The most widely used oral rabies vaccine viruses in these countries were SAD (Street Alabama D...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0408-7
更新日期：2009-01-01 00:00:00
abstract：:Adult Balb/c mice were passively immunized with monoclonal antibodies (100 micrograms/mouse) raised against tick-borne encephalitis (TBE) virus then challenged 24 hours later s.c. with 10 LD50 of TBE virus (Nëudorfl isolate). None of the mice showed evidence of premature death although all except one of the monoclonal...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310983
更新日期：1987-01-01 00:00:00
abstract：:The genome of adenovirus (AV) types 1, 2, and 5 is known to be very variable as evidenced by the great number of genome types described (36, 61, and 35 for AV1, 2, and 5 respectively). Physical maps were constructed for nearly all of the restriction or R-variants by biochemical methods and by adapting restriction frag...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718606
更新日期：1996-01-01 00:00:00
abstract：:We observed expression of envelope gp46 protein on an HTLV-II-producing T-cell line (Mo) cells by an immunoelectron microscopical method using a monoclonal antibody against HTLV-II gp46. gp46 reactivity was observed on virus-like particles, extracellular vesicles, cell membrane, and partially in nuclear membrane and e...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322755
更新日期：1995-01-01 00:00:00
abstract：:Nanoviruses are multi-component plant-infecting single-stranded DNA viruses. Using a viral metagenomics-informed approach, a new nanovirus and two associated alphasatellite molecules have been identified in an uncultivated asymptomatic Vicia cracca plant in the Rhône region of France. This novel nanovirus genome inclu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3634-4
更新日期：2018-03-01 00:00:00
abstract：:Molecular weights of six major polypeptides of infectious bronchitis virus (IBV) are: 1. 75,000; 2. 50,000; 3. 45,000; 4. 35,000; 5. 28,000 or 24,000, and 6. 22,000 dalton. According to the mobility of protein 5 the polypeptide patterns of IBV serotypes fall into two main categories. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315022
更新日期：1979-01-01 00:00:00
abstract：:Using random amplification and high-throughput sequencing technology a novel picornavirus with dicistronic genome organization and genetically related to canine picodicistrovirus (genus Dicipivirus, family Picornaviridae) was identified and characterized in Northern white-breasted hedgehogs. Hedgehog dicipivirus (hedg...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3565-0
更新日期：2018-01-01 00:00:00
abstract：:Avian Tembusu virus (ATV), an emerging virus that mainly infects laying and breeding ducks in China, has caused severe economic loss in duck industry. However, there have been no reports about host innate immune responses during ATV infection and its correlation with clinical signs or pathology. To identify the roles ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2621-x
更新日期：2016-01-01 00:00:00
abstract：:We investigated the cis-acting sequences of the promoter regulating the gene encoding the glycoprotein C (gC) of bovine herpesvirus 1 (BoHV-1), a gene of the gamma1 class. S1 nuclease protection assays revealed that gC transcription initiated predominantly at position C18281 of the viral genome, corresponding to 21 an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0898-z
更新日期：2003-01-01 00:00:00
abstract：:An assay to characterize plasma human immunodeficiency virus 1 (HIV-1) sequences for patients with low viral loads was developed by combining the selective binding of anti-CD44 MicroBeads with a nested RT-PCR targeting the env C2V4 region. Sequences were obtained from 10 of 20 HIV+ patients who had viral loads below 4...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0659-3
更新日期：2010-06-01 00:00:00
abstract：:To evaluate the variation of Potato mop-top pomovirus from potato fields, 21 isolates were collected from different Danish locations. Reverse transcription-polymerase chain reaction-restriction fragment length polymorphism (RT-PCR-RFLP) of regions of RNA2 was performed for all 21 isolates resulting in the establishmen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0926-z
更新日期：2003-02-01 00:00:00
abstract：:Avian influenza viruses of H3N8, H11N4, H7N7, H8N4, and H5N3 infected mink by contact. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309274
更新日期：1983-01-01 00:00:00
abstract：:Exposure of human lung fibroblasts to human cytomegalovirus (HCMV) stimulated a rapid increase in the release of [3H] from cells prelabelled with radiolabelled arachidonic acid ([3H]AA). Maximum stimulation of [3H] release was observed at 20 min postinfection and was quantitatively similar to that induced by the tumor...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316678
更新日期：1990-01-01 00:00:00
abstract：:We investigated the transforming domain of a recently isolated feline sarcoma virus (TP1-FeSV) which encodes a fgr-related tyrosine kinase expressed as a gag-fgr fusion protein. The gag portion was removed and replication-competent expression vectors (RCAS) with inserted v-fgr sequences were established. Chicken embry...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01379113
更新日期：1994-01-01 00:00:00
abstract：:The numbers of the carbohydrate side chains have been analyzed on a series of subtype 7 hemagglutinins of influenza A virus. The procedure employed involves growth of the virus in the presence of the trimming inhibitor N-methyl-1-desoxynojirimycin followed by controlled removal of the resulting mannose-rich oligosacch...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314433
更新日期：1987-01-01 00:00:00
abstract：:Chilli veinal mottle virus (ChiVMV) is an important plant pathogen with a wide host range. The genetic structure of ChiVMV was investigated by analyzing the coat protein (CP) genes of 87 ChiVMV isolates from seven Asian regions. Pairwise F ST values between ChiVMV populations ranged from 0.108 to 0.681, indicating a s...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2761-7
更新日期：2016-05-01 00:00:00
abstract：:A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection. The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR was performed. A common primer set for S-RNA of the Simbu serogroup of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0838-y
更新日期：2002-08-01 00:00:00
abstract：:Multidrug-resistant uropathogenic Escherichia coli (UPEC) is increasing gradually on a worldwide scale. We therefore examined the possibility of bacteriophage (phage) therapy for urinary tract infections (UTIs) caused by the UPEC strains as an alternative to chemotherapy. In addition to the well-known T4 phage, KEP10,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0031-4
更新日期：2008-01-01 00:00:00
abstract：:Gangliosides from different cells were tested for their inhibiting activity on VSV attachment to CER cells and goose erythrocytes. This inhibiting activity was enhanced when gangliosides were inserted into liposomes, in particular if containing phosphatidylserine. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310981
更新日期：1987-01-01 00:00:00
abstract：:Algeria is the largest country of Africa, with a population of 40 million inhabitants living in disparate environments from the Sahara to the large cities of the Mediterranean coast. The molecular epidemiology of hepatitis B virus (HBV) variants has been partially described, but variations in the seroprevalence of HBV...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3284-6
更新日期：2017-06-01 00:00:00
abstract：:This report describes the first isolation and partial characterization of a herpesvirus from the harbor seal (Phoca vitulina). The virus was isolated during a disease outbreak in a group of young seals nursed in a seal orphanage in The Netherlands. Almost half of the seals died with symptoms of acute pneumonia and foc...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309828
更新日期：1985-01-01 00:00:00
abstract：:A novel myovirus, vB_PagM_AAM22 (AAM22), was isolated in Lithuania using Pantoea agglomerans as the host for phage propagation. The 49,744-bp genome of AAM22 has a G + C content of 48.4% and contains 96 probable protein-encoding genes and no genes for tRNA. In total, 34 ORFs were given a putative functional annotation...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04705-4
更新日期：2020-09-01 00:00:00
abstract：:Human cytomegalovirus disappeared from cultures continuously exposed to interferon. When interferon was removed, high titers of virus reappeared. Cytomegalovirus was thus able to persist in potentially infectious form in cells protected by interferon. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309276
更新日期：1983-01-01 00:00:00
abstract：:Heterogeneity of 9 feline herpesvirus type 1 (FHV-1) strains consisting of the prototype C27 strain, one French isolate, six Japanese isolates, and the attenuated vaccine F2 strain was examined by biological, immunological, and molecular biological methods. No significant difference was observed in virus growth and an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309701
更新日期：1992-01-01 00:00:00
abstract：:Tobacco vein banding mosaic virus (TVBMV) is of increasing importance in tobacco production. Knowledge of the genetic structure and variability of the virus population is vital for developing sustainable management. In this study, 24 new TVBMV isolates from Sichuan Province together with 46 previous isolates were stud...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04342-6
更新日期：2019-10-01 00:00:00
abstract：:The complete genome of a novel mycovirus, Puccinia striiformis mitovirus 1 (PsMV1), derived from the wheat stripe rust fungus Puccinia striiformis strain SCSN-10, was sequenced and analyzed. The full-length cDNA sequence is 2496 bp in length with a predicted AU content of 57.65% in the genomic RNA. Sequence analysis i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-04134-4
更新日期：2019-03-01 00:00:00
abstract：:To establish an animal model for the newly identified Marmota Himalayana hepatovirus, MHHAV, so as to develop a better understanding of the infection of hepatitis A viruses. Five experimental woodchucks (Marmota monax) were inoculated intravenously with the purified MHHAV from wild woodchuck feces. One animal injected...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3715-z
更新日期：2018-05-01 00:00:00
