The isolation and partial characterization of a highly pathogenic herpesvirus from the harbor seal (Phoca vitulina).

Abstract:

:This report describes the first isolation and partial characterization of a herpesvirus from the harbor seal (Phoca vitulina). The virus was isolated during a disease outbreak in a group of young seals nursed in a seal orphanage in The Netherlands. Almost half of the seals died with symptoms of acute pneumonia and focal hepatitis and the virus was isolated of organs of most of the dead animals. Seven out of ten seals of which paired serum samples were obtained showed seroconversion in a virus neutralization test during this outbreak. The virus was tentatively characterized as a herpesvirus (seal herpesvirus: SeHV or phocid herpesvirus 1) on the basis of its characteristic morphology in electron microscopy, buoyant density in sucrose, sensitivity to ether and heat treatment and its antigenic relationship with other probable members of the Alphaherpesvirinae subfamily. The virus caused cytopathic changes within 24 hours after inoculation in seal kidney cells, consisting of a focal rounding of cells and syncytium formation. No cytopathic changes were observed in the cells of nine other mammalian species tested.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Osterhaus AD,Yang H,Spijkers HE,Groen J,Teppema JS,van Steenis G

doi

10.1007/BF01309828

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

239-51

issue

3-4

eissn

0304-8608

issn

1432-8798

journal_volume

86

pub_type

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