当前位置： SCI文献检索 > ARCHIVES OF VIROLOGY期刊下所有文献 > Cellular oncogene activation by human cytomegalovirus. Lack of correlation with virus infectivity and immediate early gene expression.

Cellular oncogene activation by human cytomegalovirus. Lack of correlation with virus infectivity and immediate early gene expression.

Abstract：

:The contribution of expression of human cytomegalovirus (HCMV) immediate early (IE) genes to the rapid and transient increase in cellular (c)-oncogene (fos, jun, myc) transcription following HCMV infection was investigated. A partial temporal overlap was observed between the increases in c-oncogene RNA levels and the increase in either transcripts from HCMV IE genes or the number of cells in which HCMV IE proteins were detected. The increases in c-oncogene RNA levels, however, slightly preceded the increase in the detection of HCMV IE transcripts or proteins. To distinguish between the temporal coincidence and a direct relationship between expression of HCMV IE genes and the increased transcription of c-oncogenes, the number of cells synthesizing HCMV IE proteins was reduced by infecting with virus stock enriched in defective particles. Alternatively, the synthesis of HCMV IE proteins was essentially eliminated by ultra-violet (UV) irradiation of virus stock or by inhibitors of protein synthesis. Virus stocks enriched in defective particles demonstrated a substantially reduced capacity to direct the synthesis of HCMV IE proteins, but were more efficient in activating c-oncogene expression than infectious virus stocks. Elimination of expression of HCMV IE genes by UV-irradiation of virus stock or by inhibiting de novo viral and/or cellular protein synthesis with cycloheximide (100 micrograms/ml) or anisomycin (100 micrograms/ml) did not eliminate the HCMV-induced increase in RNA levels of c-oncogenes. These data indicate that activation of these early response cellular genes is independent from de novo expression of HCMV IE proteins, and possibly involves biologically active virion proteins that are related to the induction of a cascade of cellular events associated with the binding of HCMV to its cellular receptor.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Boldogh I,AbuBakar S,Millinoff D,Deng CZ,Albrecht T

doi

10.1007/BF01314027

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

163-77

issue

3-4

eissn

0304-8608

issn

1432-8798

journal_volume

118

pub_type

杂志文章

在线工具

Cellular oncogene activation by human cytomegalovirus. Lack of correlation with virus infectivity and immediate early gene expression.