Abstract：
:Mycoviruses are viruses that infect fungi. Recently, mycovirus-like RNAs were sequenced from the fungus Phakopsora pachyrhizi, the causal agent of soybean rust. One of the RNAs appeared to represent a novel mycovirus and was designated Phakopsora pachyrhizi virus 2383 (PpV2383). The genome of PpV2383 resembles Saccharomyces cerevisiae virus L-A, a double-stranded (ds) RNA mycovirus of yeast. PpV2383 encodes two major, overlapping open reading frames with similarity to gag (capsid protein) and pol (RNA-dependent RNA polymerase), and a -1 ribosomal frameshift is necessary for the translation of a gag-pol fusion protein. Phylogenetic analysis of pol relates PpV2383 to members of the family Totiviridae, including L-A. Because the obligate biotrophic nature of P. pachyrhizi makes it genetically intractable for in vivo analysis and because PpV2383 is similar to L-A, we synthesized a DNA clone of PpV2383 and tested its infectivity in yeast cells. PpV2383 RNA was successfully expressed in yeast, and mass spectrometry confirmed the translation of gag and gag-pol fusion proteins. There was, however, no production of PpV2383 dsRNA, the evidence of viral replication. Neither the presence of endogenous L-A nor the substitution of the 5' and 3' untranslated regions with those from L-A was sufficient to rescue replication of PpV2383. Nevertheless, the proof of transcription and translation from the clone in vivo are steps toward confirming that PpV2383 is a mycovirus. Further development of a surrogate biological system for the study of rust mycoviruses is necessary, and such research may facilitate biological control of rust diseases.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Cooper B,Campbell KB,Garrett WMdoi
10.1007/s00705-015-2639-0subject
Has Abstractpub_date
2016-01-01 00:00:00pages
111-23issue
1eissn
0304-8608issn
1432-8798pii
10.1007/s00705-015-2639-0journal_volume
161pub_type
杂志文章abstract：:The ICP34.5 gene from HSV-2 strain 333 was cloned and, when expressed in Vero cells, enhanced the efficiency and extent of glycoprotein processing of glycoprotein C (gC1), a representative viral glycoprotein, during infection with HSV-1 SP7. The ICP34.5 from HSV-1 SP7 limits the extent and efficiency of viral glycopro...
journal_title：Archives of virology
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doi：10.1007/s00705-009-0341-9
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journal_title：Archives of virology
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doi：10.1007/BF01310767
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abstract：:The nucleotide sequence of the granulin gene of Choristoneura fumiferana granulovirus (CfGV) was determined. The gene encodes a protein of 248 amino acids with a predicted Mr of 29.299 kDa. The granulin genes of Trichoplusia ni, Pieris brassicae and Cryptophlebia leucotreta granuloviruses showed homologies ranging fro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050181
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abstract：:In infants, hepatitis B virus (HBV) infections are mainly acquired by mother-to-child transmission (MTCT). Current tests for the presence of HBV markers at birth can neither confirm nor exclude MTCT. The aim of this study was to find an early diagnostic marker of HBV MTCT. From 2011 to 2016, we studied a total of 5999...
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01321010
更新日期：1988-01-01 00:00:00
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doi：10.1007/s00705-005-0532-y
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pub_type： 杂志文章
doi：10.1007/BF01318240
更新日期：1975-01-01 00:00:00
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doi：10.1007/BF01310532
更新日期：1990-01-01 00:00:00
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更新日期：2016-08-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01378643
更新日期：1993-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0219-2
更新日期：2008-01-01 00:00:00
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更新日期：2008-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-03-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：2019-03-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1467-8
更新日期：2013-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：1998-01-01 00:00:00
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更新日期：2016-12-01 00:00:00
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更新日期：2011-04-01 00:00:00
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更新日期：2014-07-01 00:00:00
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journal_title：Archives of virology
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journal_title：Archives of virology
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更新日期：1977-01-01 00:00:00
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更新日期：2019-10-01 00:00:00
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journal_title：Archives of virology
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更新日期：1994-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3574-z
更新日期：2018-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01319227
更新日期：1991-01-01 00:00:00
abstract：:We have examined by immunofluorescent antibody staining technique the expression of herpes simplex virus type 1 (HSV-1) in organ cultures of the normal human oral mucosa. The expression of HSV-1 antigen was found selectively in the epithelial cell layers in relatively undifferentiated states such as basal layer and lo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310061
更新日期：1987-01-01 00:00:00
