Expression of a synthetic rust fungal virus cDNA in yeast.


:Mycoviruses are viruses that infect fungi. Recently, mycovirus-like RNAs were sequenced from the fungus Phakopsora pachyrhizi, the causal agent of soybean rust. One of the RNAs appeared to represent a novel mycovirus and was designated Phakopsora pachyrhizi virus 2383 (PpV2383). The genome of PpV2383 resembles Saccharomyces cerevisiae virus L-A, a double-stranded (ds) RNA mycovirus of yeast. PpV2383 encodes two major, overlapping open reading frames with similarity to gag (capsid protein) and pol (RNA-dependent RNA polymerase), and a -1 ribosomal frameshift is necessary for the translation of a gag-pol fusion protein. Phylogenetic analysis of pol relates PpV2383 to members of the family Totiviridae, including L-A. Because the obligate biotrophic nature of P. pachyrhizi makes it genetically intractable for in vivo analysis and because PpV2383 is similar to L-A, we synthesized a DNA clone of PpV2383 and tested its infectivity in yeast cells. PpV2383 RNA was successfully expressed in yeast, and mass spectrometry confirmed the translation of gag and gag-pol fusion proteins. There was, however, no production of PpV2383 dsRNA, the evidence of viral replication. Neither the presence of endogenous L-A nor the substitution of the 5' and 3' untranslated regions with those from L-A was sufficient to rescue replication of PpV2383. Nevertheless, the proof of transcription and translation from the clone in vivo are steps toward confirming that PpV2383 is a mycovirus. Further development of a surrogate biological system for the study of rust mycoviruses is necessary, and such research may facilitate biological control of rust diseases.


Arch Virol


Archives of virology


Cooper B,Campbell KB,Garrett WM




Has Abstract


2016-01-01 00:00:00














  • An attenuation mechanism of Newcastle disease vaccine strain TCND.

    abstract::To provide information on the mechanism of attenuation of a Newcastle disease vaccine strain, TCND, we compared it with the parental virulent strain California 11,914 (CAL) biologically and genetically. It was found that TCND bore the fusion protein of virulent type, consisting of a pair of dibasic amino acid residues...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Takakuwa H,Ito T,Takada A,Okazaki K,Kida H

    更新日期:1998-01-01 00:00:00

  • Double-stranded-RNA-specific adenosine deaminase 1 (ADAR1) is proposed to contribute to the adaptation of equine infectious anemia virus from horses to donkeys.

    abstract::Equine infectious anemia virus (EIAV) is a member of the genus Lentivirus of the family Retroviridae. Horses are the most susceptible equids to EIAV infection and are therefore the primary hosts of this virus. In contrast, infected donkeys do not develop clinically active equine infectious anemia (EIA). This phenomeno...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Tang YD,Zhang X,Na L,Wang XF,Fu LH,Zhu CH,Wang X,Zhou JH

    更新日期:2016-10-01 00:00:00

  • Molecular characterization of two genotypes of a new polerovirus infecting brassicas in China.

    abstract::The genomic RNA sequences of two genotypes of a brassica-infecting polerovirus from China were determined. Sequence analysis revealed that the virus was closely related to but significantly different from turnip yellows virus (TuYV). This virus and other poleroviruses, including TuYV, had less than 90% amino acid sequ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Xiang HY,Dong SW,Shang QX,Zhou CJ,Li DW,Yu JL,Han CG

    更新日期:2011-12-01 00:00:00

  • Hepatitis C virus infection in mixed cryoglobulinemia and B-cell non-Hodgkin's lymphoma: evidence for a pathogenetic role.

    abstract::We investigated the pathogenetic relevance of hepatitis C virus (HCV) infection in mixed cryoglobulinemia (MC) with or without complicating B-cell Non-Hodgkin's lymphoma (NHL) in comparison with other immunological and lymphoproliferative disorders. The following groups of patients were studied: A) 25 patients with MC...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Zignego AL,Ferri C,Giannini C,La Civita L,Careccia G,Longombardo G,Bellesi G,Caracciolo F,Thiers V,Gentilini P

    更新日期:1997-01-01 00:00:00

  • Striking differences in the biological and molecular properties of onion and garlic isolates of onion yellow dwarf virus.

    abstract::Complete nucleotide (nt) and deduced amino acid sequences of two onion yellow dwarf virus (OYDV) isolates showing mild and severe symptoms in onion but being unable to infect garlic were determined. The genomes consisted of 10,459 and 10,461 nt (without the 3' poly(A) tail) and were 92.2 % identical. Comparison of the...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Celli MG,Torrico AK,Kiehr M,Conci VC

    更新日期:2013-06-01 00:00:00

  • Proteome analysis of reticuloendotheliosis-virus-infected chicken embryo fibroblast cells through iTRAQ-based quantitative proteomics.

    abstract::Reticuloendotheliosis virus (REV) is an important representative avian retrovirus. To improve our understanding of the host cellular responses to virus infection and the pathogenesis of REV infection, we applied isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with multidimensional liqui...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Hu F,Li Y,Yu K,Ma X,Liu C,Guo X,Song M,Wu J,Huang B

    更新日期:2019-12-01 00:00:00

  • Temperature sensitivity of herpes simplex virus type 1 is a tissue-dependent phenomenon.

    abstract::The temperature sensitivity of herpes simplex virus type 1 (HSV-1) was assessed in primary cultures of mouse central nervous system (MNS) cells and mouse embryo cells (MEC). Infectious yields were determined and the ultrastructural morphogenesis of HSV-1 particles was compared following incubation at 37 or 40.5 degree...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Cole NL

    更新日期:1992-01-01 00:00:00

  • Transmission network characteristics based on env and gag sequences from MSM during acute HIV-1 infection in Beijing, China.

    abstract::Molecular epidemiology can be used to identify human immunodeficiency virus (HIV) transmission clusters, usually using pol sequence for analysis. In the present study, we explored appropriate parameters to construct a simple network using HIV env and gag sequences instead of pol sequences for constructing a phylogenet...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Zhang Z,Dai L,Jiang Y,Feng K,Liu L,Xia W,Yu F,Yao J,Xing W,Sun L,Zhang T,Wu H,Su B,Qiu M

    更新日期:2017-11-01 00:00:00

  • Passive immunization of mice with monoclonal antibodies raised against tick-borne encephalitis virus. Brief report.

    abstract::Adult Balb/c mice were passively immunized with monoclonal antibodies (100 micrograms/mouse) raised against tick-borne encephalitis (TBE) virus then challenged 24 hours later s.c. with 10 LD50 of TBE virus (Nëudorfl isolate). None of the mice showed evidence of premature death although all except one of the monoclonal...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Phillpotts RJ,Stephenson JR,Porterfield JS

    更新日期:1987-01-01 00:00:00

  • Mouse neurotropic recombinants of influenza A viruses.

    abstract::Recombinants with known gene constellations between fowl plague virus (FPV) and various prototype influenza virus strains have been examined for neurovirulence in suckling mice. Strongly neurotropic recombinants were obtained from crosses between FPV and the strains virus N, Hong Kong, and PR8, but not between FPV and...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Bonin J,Scholtissek C

    更新日期:1983-01-01 00:00:00

  • Occult hepatitis B virus infection in Korean patients with isolated anti-HBc.

    abstract::Serological detection of isolated anti-hepatitis B core antibody (anti-HBc) can occur in various scenarios, but the most clinically relevant situation is occult hepatitis B virus (HBV) infection (OBI). The purpose of this study was to evaluate the prevalence and clinical relevance of isolated anti-HBc and of OBI with ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Kang SY,Kim MH,Lee WI

    更新日期:2014-02-01 00:00:00

  • The effects of gamma interferon on replication of foot-and-mouth disease virus in persistently infected bovine cells.

    abstract::Foot-and-mouth disease virus (FMDV) causes a highly contagious viral disease of cloven-hoofed animals, which has a considerable socio-economic impact on the countries affected. In addition, persistent infection can occur following clinical or sub-clinical disease in either vaccinated or non-vaccinated cattle. The mech...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Zhang ZD,Hutching G,Kitching P,Alexandersen S

    更新日期:2002-11-01 00:00:00

  • The complete nucleotide sequence of the barley yellow dwarf GPV isolate from China shows that it is a new member of the genus Polerovirus.

    abstract::The complete nucleotide sequence of the ssRNA genome of a Chinese GPV isolate of barley yellow dwarf virus (BYDV) was determined. It comprised 5673 nucleotides, and the deduced genome organization resembled that of members of the genus Polerovirus. It was most closely related to cereal yellow dwarf virus-RPV (77% nt i...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Zhang W,Cheng Z,Xu L,Wu M,Waterhouse P,Zhou G,Li S

    更新日期:2009-01-01 00:00:00

  • Fowlpox virus polypeptides: sequential appearance and virion associated polypeptides.

    abstract::The polypeptides associated with fowlpox virus (FPV) infection of chicken embryo skin (CES) cells were examined by metabolic labelling with [35S]-methionine and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Polypeptide synthesis was followed over the first 48 hours post infection, as this was s...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Prideaux CT,Boyle DB

    更新日期:1987-01-01 00:00:00

  • Phylogenetic analysis of Vietnamese isolates of feline immunodeficiency virus: genetic diversity of subtype C.

    abstract::Phylogenetic relationships of novel Vietnamese strains of feline immunodeficiency virus (FIV) were analysed. One Vietnamese strain was found to cluster with subtype D, which was previously known only in Japan, while the other seven strains were placed with members of subtype C. Calculation of the relative numbers of m...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Nakamura K,Suzuki Y,Ikeo K,Ikeda Y,Sato E,Nguyen NT,Gojobori T,Mikami T,Miyazawa T

    更新日期:2003-04-01 00:00:00

  • Characterization of the complete genome of ribgrass mosaic virus isolated from Plantago major L. from New Zealand and Actinidia spp. from China.

    abstract::The complete genomes of tobamovirus isolates from Plantago major L. from New Zealand (NZ-439), Plantago sp. from Germany (Kons 1105), Actinidia chinensis (Actinidia-AC) and A. deliciosa (Actinidia-AD) from China were sequenced and compared to previously published tobamovirus genomes. Their genome organization and phyl...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Chavan RR,Cohen D,Blouin AG,Pearson MN

    更新日期:2012-07-01 00:00:00

  • Characterisation of Banana streak Mysore virus and evidence that its DNA is integrated in the B genome of cultivated Musa.

    abstract::We have sequenced the complete genome of an isolate of Banana streak virus from banana cv. 'Mysore' and show that it is sufficiently different from a previously characterised isolate from cv. 'Obino l'Ewai' to warrant recognition as a distinct species, for which the name Banana streak Mysore virus (BSMysV) is proposed...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Geering AD,Pooggin MM,Olszewski NE,Lockhart BE,Thomas JE

    更新日期:2005-04-01 00:00:00

  • Identification and transcriptional analysis of pseudorabies virus UL6 to UL12 genes.

    abstract::We determined the nucleotide sequence of an 11059 bp fragment of the pseudorabies virus genome located in the right part of genomic BamHI fragment 3 and the adjacent part of BamHI fragment 6. Within this region eight open reading frames were identified whose deduced amino acid sequences exhibited homology to the UL6, ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Dijkstra JM,Fuchs W,Mettenleiter TC,Klupp BG

    更新日期:1997-01-01 00:00:00

  • Characterization of a recombinant encephalomyocarditis virus expressing the enhanced green fluorescent protein.

    abstract::A recombinant encephalomyocarditis virus (rEMCV2887A-egfp) expressing the enhanced green fluorescent protein (EGFP) was produced. The EGFP gene was inserted in frame within the leader protein coding sequence of a full-length cDNA clone of EMCV. RNA transcripts derived from the recombinant full-length cDNA were synthes...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Hammoumi S,Cruciere C,Guy M,Boutrouille A,Messiaen S,Lecollinet S,Bakkali-Kassimi L

    更新日期:2006-09-01 00:00:00

  • Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome.

    abstract::Herpes simplex virus type 1 (HSV-1) is a ubiquitous human pathogen of skin and mucous membranes. In the present study, the genome of the HSV-1 F strain was cloned as an infectious bacterial artificial chromosome (BAC) clone without any deletions of the viral genes. Additionally, a firefly luciferase cassette was inser...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Li Y,Wang S,Zhu H,Zheng C

    更新日期:2011-12-01 00:00:00

  • Deletion of a decoy epitope in porcine circovirus 2 (PCV2) capsid protein affects the protective immune response in mice.

    abstract::The swine pathogen porcine circovirus type 2 (PCV2) causes significant economic damage worldwide. The PCV2 capsid (CP) residues 169-STIDYFQPNNKR-180 have been identified as a decoy epitope that diverts the host immune response away from protective epitopes. However, the decoy epitope may include important linear or co...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Kim K,Shin M,Hahn TW

    更新日期:2020-12-01 00:00:00

  • IL-17A in hepatitis B infection: friend or foe?

    abstract::Hepatitis B virus (HBV) is one of the most prevalent and infectious agents that leads to liver disease in humans. Five clinical forms of HBV infection exist, including fulminant, acute, chronic, asymptomatic and occult. The chronic, asymptomatic and occult forms are long-term infections that can lead to hepatocellular...

    journal_title:Archives of virology

    pub_type: 杂志文章,评审


    authors: Arababadi MK,Bidaki MZ,Kennedy D

    更新日期:2014-08-01 00:00:00

  • Prevalence of pigeon rotavirus infections: animal exhibitions as a risk factor for pigeon flocks.

    abstract::A total of 289 cloacal swabs from pigeons from 29 different breeders in Germany were collected. In addition, samples from pigeons exhibited at shows were collected. The detailed health status of the pigeon flocks was recorded. Samples were analysed for the presence of the recently discovered pigeon rotavirus and pigeo...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Harzer M,Heenemann K,Sieg M,Vahlenkamp T,Freick M,Rückner A

    更新日期:2021-01-01 00:00:00

  • Merkel cell polyomavirus IgG antibody levels are associated with progression to AIDS among HIV-infected individuals.

    abstract::The association of Merkel cell polyomavirus (MCPyV) with Merkel cell carcinoma (MCC) in immunocompromised individuals has been revealed in a number of surveys. The study of MCPyV specific antibody titers and viral loads in such patients has a great attraction for research groups interested in viral reactivation. In th...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Vahabpour R,Nasimi M,Naderi N,Salehi-Vaziri M,Mohajel N,Sadeghi F,Keyvani H,Monavari SH

    更新日期:2017-04-01 00:00:00

  • Perpetuation of influenza A viruses in Alaskan waterfowl reservoirs.

    abstract::To provide information on the mechanism of perpetuation of influenza viruses among waterfowl reservoirs in nature, virological surveillance was carried out in Alaska during their breeding season in summer from 1991 to 1994. Influenza viruses were isolated mainly from fecal samples of dabbling ducks in their nesting pl...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Ito T,Okazaki K,Kawaoka Y,Takada A,Webster RG,Kida H

    更新日期:1995-01-01 00:00:00

  • Coat protein gene sequence of an Austrian isolate of grapevine fanleaf virus.

    abstract::The nucleotide sequence of the coat protein cistron of an Austrian isolate of grapevine fanleaf virus (GFLV-FC) from Vitis vinifera cv. French Colombard was determined. It shows small differences at the RNA level as well as at the protein level compared to the sequences of already published grapevine fanleaf virus str...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Brandt S,Ibl M,Himmler G

    更新日期:1995-01-01 00:00:00

  • Rapid detection of orf virus by loop-mediated isothermal amplification based on the DNA polymerase gene.

    abstract::At present, there are no effective antiviral treatments available for contagious ecthyma, and rapid diagnosis is therefore critical for effective control of the disease. Recently, the invention of a novel LAMP technique that can rapidly amplify nucleic acids with high specificity and sensitivity under isothermal condi...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Li J,Song D,He W,Bao Y,Lu R,Su G,Wang G,Lu H,Zhao K,Gao F

    更新日期:2013-04-01 00:00:00

  • Molecular characterization of yam virus X, a new potexvirus infecting yams (Dioscorea spp) and evidence for the existence of at least three distinct potexviruses infecting yams.

    abstract::The genome of yam virus X (YVX), a new member of the genus Potexvirus from yam (Dioscorea trifida), was completely sequenced. Structural and phylogenetic analysis showed that the closest relative of YVX is nerine virus X. A prevalence study found YVX only in plants maintained in Guadeloupe and showed that it also infe...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Mambole IA,Bonheur L,Dumas LS,Filloux D,Gomez RM,Faure C,Lange D,Anzala F,Pavis C,Marais A,Roumagnac P,Candresse T,Teycheney PY

    更新日期:2014-12-01 00:00:00

  • Comparative study with monospecific and monoclonal antibodies against a 65 K human cytomegalovirus protein.

    abstract::Immunofluorescence assay using monospecific and monoclonal antibodies to the 65 K major protein of human cytomegalovirus (HCMV) was carried out to monitor the expression of this protein in infected cells. Regardless of differences in the reactivity of the monoclonal antibodies, as determined by immunoblotting and immu...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Shimokawa K,Bin X,Furukawa T

    更新日期:1988-01-01 00:00:00

  • Novel seadornavirus (family Reoviridae) related to Banna virus in Europe.

    abstract::Banna virus, whose genome is composed of 12 segments of double-stranded RNA, is a member of the genus Seadornavirus in the family Reoviridae and is thought to be an emerging mosquito-transmitted human pathogen in Southeast Asia. A novel phylogenetic relative of Banna virus (Balaton virus, BALV, JX947843-JX947850 and K...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Reuter G,Boros A,Delwart E,Pankovics P

    更新日期:2013-10-01 00:00:00