Expression of a synthetic rust fungal virus cDNA in yeast.


:Mycoviruses are viruses that infect fungi. Recently, mycovirus-like RNAs were sequenced from the fungus Phakopsora pachyrhizi, the causal agent of soybean rust. One of the RNAs appeared to represent a novel mycovirus and was designated Phakopsora pachyrhizi virus 2383 (PpV2383). The genome of PpV2383 resembles Saccharomyces cerevisiae virus L-A, a double-stranded (ds) RNA mycovirus of yeast. PpV2383 encodes two major, overlapping open reading frames with similarity to gag (capsid protein) and pol (RNA-dependent RNA polymerase), and a -1 ribosomal frameshift is necessary for the translation of a gag-pol fusion protein. Phylogenetic analysis of pol relates PpV2383 to members of the family Totiviridae, including L-A. Because the obligate biotrophic nature of P. pachyrhizi makes it genetically intractable for in vivo analysis and because PpV2383 is similar to L-A, we synthesized a DNA clone of PpV2383 and tested its infectivity in yeast cells. PpV2383 RNA was successfully expressed in yeast, and mass spectrometry confirmed the translation of gag and gag-pol fusion proteins. There was, however, no production of PpV2383 dsRNA, the evidence of viral replication. Neither the presence of endogenous L-A nor the substitution of the 5' and 3' untranslated regions with those from L-A was sufficient to rescue replication of PpV2383. Nevertheless, the proof of transcription and translation from the clone in vivo are steps toward confirming that PpV2383 is a mycovirus. Further development of a surrogate biological system for the study of rust mycoviruses is necessary, and such research may facilitate biological control of rust diseases.


Arch Virol


Archives of virology


Cooper B,Campbell KB,Garrett WM




Has Abstract


2016-01-01 00:00:00














  • Purification of mengovirus by freon extraction and chromatography on protein-coated controlled pore glass.

    abstract::Mengovirus, extracted from infected L-cell cultures with Freon 113 and concentrated from the aqueous phase with polyethyleneglycol, was chromatographed on protein-coated controlled pore glass (CPG). The covalent binding of protein to CPG is described. Further purification and concentration of mengovirus was achieved b...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Gschwender HH,Traub P

    更新日期:1978-01-01 00:00:00

  • Characterization of the complete genome of ribgrass mosaic virus isolated from Plantago major L. from New Zealand and Actinidia spp. from China.

    abstract::The complete genomes of tobamovirus isolates from Plantago major L. from New Zealand (NZ-439), Plantago sp. from Germany (Kons 1105), Actinidia chinensis (Actinidia-AC) and A. deliciosa (Actinidia-AD) from China were sequenced and compared to previously published tobamovirus genomes. Their genome organization and phyl...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Chavan RR,Cohen D,Blouin AG,Pearson MN

    更新日期:2012-07-01 00:00:00

  • Analysis of the structure of human papilloma virus DNA.

    abstract::Human papilloma virus DNA has been examined for heterogeneity by electron microscopy, reassociation kinetics, and restriction endonuclease digestion. No heterogeneity in contour length was detected. The reassociation rate of papilloma DNA isolated from pooled warts was consistent with that expected for a homogeneous, ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: DeLap RJ,Yanagi K,Rush MG

    更新日期:1977-01-01 00:00:00

  • Identification of a novel bipartite nuclear localization signal in the DNA polymerase of the betabaculovirus Pieris rapae granulovirus.

    abstract::DNA polymerase (DNApol) is highly conserved in all baculoviruses and plays an essential role in viral DNA replication. Previous results showed that the DNApol of the betabaculovirus Pieris rapae granulovirus (PiraGV) can localize in the nucleus. However, it is not clear how the DNApol is transported into the nucleus. ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Li P,Wu L,Chen G

    更新日期:2019-03-01 00:00:00

  • Characterization of echovirus 25 (ECV 25) in the VP1/2A gene junction region. Brief report.

    abstract::Genetic relationships among echovirus type 25 (ECV 25) isolates associated with aseptic meningitis in Germany 1997/98 and a 40-year-old ECV 25 prototype strain were investigated using RT-PCR and direct sequencing the VP1/2A gene junction region. Sequences were compared to each other and to non-polio enterovirus repres...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Künkel U,Schreier E

    更新日期:1999-01-01 00:00:00

  • Modulation of lethal and persistent rat parvovirus infection by antibody.

    abstract::Two day-old athymic (rnu/rnu) and euthymic (rnu/+) rat pups nursing immune or non-immune dams were inoculated oronasally with the Yale strain of rat virus (RV-Y). All athymic and euthymic pups (57/57) from immune dams remained clinically normal, whereas 51 of 66 athymic and euthymic pups from non-immune dams died with...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Gaertner DJ,Jacoby RO,Paturzo FX,Johnson EA,Brandsma JL,Smith AL

    更新日期:1991-01-01 00:00:00

  • Molecular detection of genotype 3 porcine hepatitis E virus in aborted fetuses and their sows.

    abstract::By reverse transcription (RT)-nested polymerase chain reaction (PCR), hepatitis E virus (HEV) RNA was simultaneously detected in the livers of aborted fetuses and in fecal and serum samples from their sows, obtained from two of 14 farms tested. Sequence and phylogenetic analyses showed that these HEVs belonged to geno...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Hosmillo M,Jeong YJ,Kim HJ,Park JG,Nayak MK,Alfajaro MM,Collantes TM,Park SJ,Ikuta K,Yunoki M,Kang MI,Park SI,Cho KO

    更新日期:2010-07-01 00:00:00

  • A novel East African monopartite begomovirus-betasatellite complex that infects Vernonia amygdalina.

    abstract::The complete genomes of a monopartite begomovirus (genus Begomovirus, family Geminiviridae) and an associated betasatellite found infecting Vernonia amygdalina Delile (family Compositae) in Uganda were cloned and sequenced. Begomoviruses isolated from two samples showed the highest nucleotide sequence identity (73.1% ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Mollel HG,Ndunguru J,Sseruwagi P,Alicai T,Colvin J,Navas-Castillo J,Fiallo-Olivé E

    更新日期:2017-04-01 00:00:00

  • The genome sequence of Brassica campestris chrysovirus 1, a novel putative plant-infecting tripartite chrysovirus.

    abstract::A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named "Brassica campestris chrysovirus 1" (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respe...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Zhang J,Zhao Z,Hu R,Guo L,Zheng L,Du Z,Wu Z,Fang S,Zhang S,Liu Y

    更新日期:2017-04-01 00:00:00

  • Bovine respiratory syncytial virus replicates minimally in bovine alveolar macrophages.

    abstract::The interaction between two different bovine respiratory syncytial virus (BRSV) strains and bovine alveolar macrophages (BAMs) was studied in vitro. Bovine respiratory syncytial virus replicated minimally in BAMs and most of the virus produced remained cell-associated. Approximately 1 out of 1,000 BAMs produced infect...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Schrijver RS,Kramps JA,Middel WG,Langedijk JP,van Oirschot JT

    更新日期:1995-01-01 00:00:00

  • The host HLA-A*02 allele is associated with the response to pegylated interferon and ribavirin in patients with chronic hepatitis C virus infection.

    abstract::Human leukocyte antigen (HLA) alleles are associated with both the progression of chronic hepatitis C (CHC) and the sustained virological response (SVR) to antiviral therapy. HLA-A*02 is the most common HLA allele in people of European/Caucasian descent and the Chinese and Japanese population. Therefore, we investigat...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Wang M,Li JS,Ping Y,Li ZQ,Wang LP,Guo Q,Zhang Z,Yue DL,Wang F,Zhang TF,Islam MS,Zhang Y

    更新日期:2015-04-01 00:00:00

  • The HIV-1 V3 domain on field isolates: participation in generation of escape virus in vivo and accessibility to neutralizing antibodies.

    abstract::The V3 domain is highly variable and induces HIV neutralizing antibodies (NA). Here we addressed the issues of 1) the participation of mutations in V3 in generation of neutralization resistant escape virus in vivo and 2) the applicability of synthetic V3 peptides corresponding to field isolates to induce neutralizing ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Arendrup M,Akerblom L,Heegaard PM,Nielsen JO,Hansen JE

    更新日期:1995-01-01 00:00:00

  • Adoptive transfer of macrophages from adult mice reduces mortality in mice infected with human enterovirus 71.

    abstract::Human enterovirus 71 (EV71) causes hand, foot and mouth disease in children under 6 years of age, and the neurological complications of this virus can lead to death. Until now, no vaccines or drugs have been available for the clinical control of this epidemic. Macrophages can engulf pathogens and mediate a series of h...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Liu J,Li X,Fan X,Ma C,Qin C,Zhang L

    更新日期:2013-02-01 00:00:00

  • The complete nucleotide sequence of the Popp (1967) strain of Marburg virus: a comparison with the Musoke (1980) strain.

    abstract::The nucleotide sequence of genomic RNA of Marburg virus strain Popp was determined. Strain Popp was isolated in 1967 during the first filoviral outbreak. The virus was purified from blood of infected guinea pigs in which it had been maintained. The length of the determined sequence was 19112 nucleotides. Amino acid se...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Bukreyev AA,Volchkov VE,Blinov VM,Dryga SA,Netesov SV

    更新日期:1995-01-01 00:00:00

  • Genetic grouping for the isolates of avian infectious bronchitis virus in Taiwan.

    abstract::In order to differentiate recent isolates of avian infectious bronchitis virus (IBV) in Taiwan, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and direct sequencing methods were used to type 25 IBV Taiwan isolates. Two conserved sequences that flank the hypervariable region I (HVR I)...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Wang CH,Tsai CT

    更新日期:1996-01-01 00:00:00

  • The complete genome sequence of an enterovirus 76 isolate in China reveals a recombination event.

    abstract::Enterovirus 76 (EV76) is a new member of species Human Enterovirus A (HEV-A). So far, the only complete genome sequence of the prototype strain from France has been available. In this study, we determined the complete nucleotide sequence of strain 04360, isolated from an acute flaccid paralysis patient in Shandong pro...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Xu A,Tao Z,Lin X,Liu Y,Zhang Y,Song L,Wang H,Yang J,Li Y,Ji F,Feng L,Zhao Z

    更新日期:2011-09-01 00:00:00

  • Detection and genetic characterization of porcine astroviruses in piglets with and without diarrhea in Thailand.

    abstract::Porcine astrovirus (PAstV) is widely distributed and highly prevalent among pigs, nevertheless its clinical significance remains unclear as it can be detected in both diarrheic and in healthy pigs. Information about the prevalence, clinical significance and molecular characterization of PAstV in Thailand is not availa...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Kumthip K,Khamrin P,Saikruang W,Kongkaew A,Vachirachewin R,Ushijima H,Maneekarn N

    更新日期:2018-07-01 00:00:00

  • T cell subset profile and inflammatory cytokine properties in the gut-associated lymphoid tissues of chickens during infectious bursal disease virus (IBDV) infection.

    abstract::While infectious bursal disease virus (IBDV) mainly targets immature B cells and causes T cell infiltration in the bursa of Fabricius (BF) of chickens, the effect of IBDV infection on the properties of T cells and relevant cytokine production in avian gut-associated lymphoid tissues (GALTs) remains unknown. Here, we s...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Ruan Y,Wang Y,Guo Y,Xiong Y,Chen M,Zhao A,Liu H

    更新日期:2020-10-01 00:00:00

  • Molecular characterization of two prunus necrotic ringspot virus isolates from Canada.

    abstract::We determined the entire RNA1, 2 and 3 sequences of two prunus necrotic ringspot virus (PNRSV) isolates, Chr3 from cherry and Pch12 from peach, obtained from an orchard in the Niagara Fruit Belt, Canada. The RNA1, 2 and 3 of the two isolates share nucleotide sequence identities of 98.6%, 98.4% and 94.5%, respectively....

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Cui H,Hong N,Wang G,Wang A

    更新日期:2012-05-01 00:00:00

  • Immune adherence hemagglutination test for detection of antibody to respiratory syncytial virus. Brief report.

    abstract::An immune adherence hemagglutination test was established for detection of antibodies to respiratory syncytial virus. The test was shown to be as specific as and much more sensitive than the neutralization and complement fixation tests. ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Kobayashi N,Noguchi Y,Kojima M,Matumoto M

    更新日期:1984-01-01 00:00:00

  • DNA vaccination induces a long-term antibody response and protective immunity against pseudorabies virus in mice.

    abstract::In order to investigate the mechanism of long-term immunity and the effect of protective immunity induced by DNA vaccination, we constructed the expression plasmid containing a pseudorabies virus (PRV) gD gene encoding an envelope glycoprotein. Intramuscular vaccination of mice with the plasmid DNA induced a strong an...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Ho TY,Hsiang CY,Hsiang CH,Chang TJ

    更新日期:1998-01-01 00:00:00

  • Phosphatidylinositol-3-kinase-Akt pathway in negative-stranded RNA virus infection: a minireview.

    abstract::The PI3K/Akt signalling pathway is a crucial signalling cascade that regulates transcription, protein translation, cell growth, proliferation, cell survival, and metabolism. During viral infection, viruses exploit a variety of cellular pathways, including the well-known PI3K/Akt signalling pathway. Conversely, cells r...

    journal_title:Archives of virology

    pub_type: 杂志文章,评审


    authors: Blanco J,Cameirao C,López MC,Muñoz-Barroso I

    更新日期:2020-10-01 00:00:00

  • Identification and sequence analyses of the granulin gene of Choristoneura fumiferana granulovirus.

    abstract::The nucleotide sequence of the granulin gene of Choristoneura fumiferana granulovirus (CfGV) was determined. The gene encodes a protein of 248 amino acids with a predicted Mr of 29.299 kDa. The granulin genes of Trichoplusia ni, Pieris brassicae and Cryptophlebia leucotreta granuloviruses showed homologies ranging fro...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Bah A,Bergeron J,Arella M,Lucarotti CJ,Guertin C

    更新日期:1997-01-01 00:00:00

  • Identification and characterization of an isolate of apple green crinkle associated virus involved in a severe disease of quince (Cydonia oblonga, Mill.).

    abstract::A quince tree showing severe symptoms of a previously undescribed viral disease occurring in northern Apulia (Italy) was analysed using high-throughput sequencing of small RNA libraries, leading to the identification of a new strain of apple green crinkle associated virus (isolate AGCaV-CYD) showing peculiar traits. R...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Morelli M,Giampetruzzi A,Laghezza L,Catalano L,Savino VN,Saldarelli P

    更新日期:2017-01-01 00:00:00

  • Comparative sequence analysis of a polymorphic region of the spike glycoprotein S1 subunit of enteric bovine coronavirus isolates.

    abstract::Complementary oligonucleotide primers which flank a 1146-nucleotide gene fragment (S1B: nt 1185 to 2333) encompassing a polymorphic region (nt 1368 to 1776) of the S1 subunit of bovine coronavirus spike glycoprotein were used for enzymatic amplification by PCR. We chose four clinical isolates, recovered from cases of ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Rekik MR,Dea S

    更新日期:1994-01-01 00:00:00

  • The analysis of clinical and laboratory data: a large outbreak of dengue fever in Chaozhou, Guangdong province, China.

    abstract::A large-scale dengue fever (DF) outbreak occurred in Chaozhou, Guangdong province, China 2015. In our study, 528 dengue-positive patient samples were collected for clinical and laboratory data analysis. 491 cases (93.0%) were primary dengue fever (PDF), 22 cases (4.2%) were dengue hemorrhagic fever (DHF) and 15 cases ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Lin F,Yang H,Zhang L,Fang SH,Zhan XF,Yang LY

    更新日期:2019-08-01 00:00:00

  • RT-PCR detection of the expression of the polymerase gene of a novel reptilian herpesvirus in tumor tissues of green turtles with fibropapilloma.

    abstract::An alpha-herpesvirus has recently been associated with green turtle fibropapilloma (FP). To further understand the etiological role of this newfound green turtle herpesvirus (GTHV) in the pathogenesis of FP, expression of GTHV polymerase ( pol) gene was determined in tumors and normal-appearing nontumor tissues and or...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Lu YA,Wang Y,Aguirre AA,Zhao ZS,Liu CY,Nerurkar VR,Yanagihara R

    更新日期:2003-06-01 00:00:00

  • Influenza A/Fujian/411/02(H3N2)-lineage viruses in Finland: genetic diversity, epidemic activity and vaccination-induced antibody response.

    abstract::The first sporadic cases of Fujian/411/02-lineage viruses were recorded in Finland in winter 2001-2002. The first protracted but low-intensity outbreak occurred here during the first half of 2003, and the second outbreak early in autumn 2003, after detection of sporadic influenza A cases in the summer. The calculated ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Ikonen N,Pyhälä R,Toivonen M,Korpela H

    更新日期:2006-02-01 00:00:00

  • Distinct usage of three C-type lectins by Japanese encephalitis virus: DC-SIGN, DC-SIGNR, and LSECtin.

    abstract::Infection with West Nile virus and dengue virus, two mosquito-borne flaviviruses, is enhanced by two calcium-dependent lectins: dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), and its related molecule (DC-SIGNR). The present study examined the relationship between Japanese enc...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Shimojima M,Takenouchi A,Shimoda H,Kimura N,Maeda K

    更新日期:2014-08-01 00:00:00

  • A mycoparasitic and opportunistic fungus is inhabited by a mycovirus.

    abstract::A novel bisegmented double-stranded RNA virus was identified in the mycoparasitic and opportunistic fungus Hypomyces chrysospermus. The RNA1 genome segment comprises 1866 bp and encodes an RNA-dependent RNA polymerase (RdRp). The RNA2 segment comprises 1822 bp and encodes a capsid protein. Phylogenetic analysis of the...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Petrzik K,Siddique AB

    更新日期:2019-10-01 00:00:00