Abstract:
:In the aim to study the function of the bovine herpesvirus 1 (BoHV-1) UL28 protein during the replicative cycle, we characterized a UL28 deletion mutant of BoHV-1, BoHV-1 Delta UL28. Productive growth of BoHV-1 Delta UL28 was only observed in a specifically engineered complementing cell line expressing the native UL28 protein, demonstrating that UL28 is essential for virus replication. UL28 deficiency did not compromised viral protein synthesis of the late class as shown by the detection of the viral alpha gene trans-inducing factor protein encoded by UL48, a gene of the gamma2 class. Southern blotting analyses of total DNA extracted from BoHV-1 Delta UL28-infected normal cells revealed that viral DNA replication was not compromised but the process of cleavage of the newly synthesized DNA was defective. Transmission electron microscopy of non-complementing BoHV-1 Delta UL28-infected cells revealed an accumulation of capsids devoid of DNA, suggesting that the DNA packaging was impaired. We conclude that the BoHV-1 UL28 protein is essential for viral replication and is necessary for the formation of mature capsid.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Desloges N,Simard Cdoi
10.1007/s00705-002-0962-8keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
623-42issue
4eissn
0304-8608issn
1432-8798journal_volume
148pub_type
杂志文章abstract::The RNAs of 4 avian rotavirus isolates were analysed by polyacrylamide gel electrophoresis. One of these isolates was made from chickens, the other three were from turkeys. The RNAs were separated into 8 or 9 bands consisting of 11 segments, ranging in molecular weight from 0.24 X 10(6) to 2.09 X 10(6). In contrast to...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01320765
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journal_title:Archives of virology
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0231-5
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3015-4
更新日期:2016-11-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2416-0
更新日期:2015-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00705-012-1225-y
更新日期:2012-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/BF01309868
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pub_type: 杂志文章
doi:10.1007/s00705-013-1670-2
更新日期:2013-08-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2017-09-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1457-x
更新日期:2013-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2718-2
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
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