Columbid circoviruses detected in free ranging pigeons from Southern Brazil: insights on PiCV evolution.

Abstract:

:Pigeon circovirus (PiCV) is taxonomically classified as a member of the Circovirus genus, family Circoviridae. The virus contains a single stranded DNA genome of approximately 2 kb, with minor length variations among different isolates. The occurrence of PiCV infections in pigeons (Columba livia) has been documented worldwide over the past 20 years; however, in Brazil there were still no reports on PiCV detection. This study identifies seven PiCV genomes recovered from domestic pigeons of South Brazil through high-throughput sequencing and shows a high frequency of PiCV infection, through quantitative real-time PCR. Phylogenetic classification was performed by maximum likelihood analysis of the full genomes, ORF V1 (Rep) and ORF C1 (Cap). The results show that either full genome or Cap based analysis allowed PiCV classification into five major clades (groups A to E), where Brazilian sequences were classified as A, C or D. Recombination analyses were carried out with Simplot and RDP4 and the results show that both Rep and Cap ORFs contain several recombination hotspots, pointing to an important role for such events in PiCV evolution.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Loiko MR,Junqueira DM,Varela APM,Tochetto C,Scheffer CM,Lima DA,Morel AP,Cerva C,Paim WP,Mayer FQ,Roehe PM

doi

10.1007/s00705-018-3990-8

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

3083-3090

issue

11

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-018-3990-8

journal_volume

163

pub_type

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