Identification of amino acids involved in a serotype and neutralization specific epitope within the s1 subunit of avian infectious bronchitis virus.

Abstract:

:Localization of neutralizing, serotype specific epitopes of infectious bronchitis virus has been difficult because these epitopes are conformationally dependent. We identified amino acids involved in a serotype specific, conformationally dependent epitope by analysis of the S1 gene of 13 monoclonal antibody-neutralization-resistant mutants. Substitutions in the predicted amino acid sequence of these mutants were located at residues 304 and/or 386. Most of the substitutions at residue 304 were from threonine to isoleucine, whereas the substitutions at residue 386 were from arginine to proline, histidine, cysteine, or tryptophan. Based on this data, it appears that AA residues at 304 and 386 on the S1 glycoprotein are involved in a virus neutralizing serotype specific epitope.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Moore KM,Jackwood MW,Hilt DA

doi

10.1007/s007050050239

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

2249-56

issue

11

eissn

0304-8608

issn

1432-8798

journal_volume

142

pub_type

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