A CD 4: immunoglobulin fusion protein with antiviral effects against HIV.

Abstract:

:Antibody-like molecules consisting of the human CD 4 extracellular domain fused to human IgG1 heavy chain constant regions were genetically constructed and expressed in a BHK cell stable transfectant. Purified chimeric antibodies bound to HIV particles as it was shown by immuno electron microscopy, inhibited fusions of HIV-1-infected cells with uninfected cells, neutralized HIV-1, and were able to inhibit the spread of a cellular HIV-1 infection in CD 4+ cells. Plaque reduction assays with CD 4(+)-transfected Hela-cells showed a comparable inhibition of HIV-1 and HIV-2. Inhibitory functions were enhanced in the presence of complement. HIV-1- and HIV-2-infected CD 4+ cells were efficiently lysed by a slow, complement-dependent mechanism, whereas uninfected CD 4+ cells and HLA-DR+ cells were not affected.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Gregersen JP,Mehdi S,Gelderblom H,Zettlmeissl G

doi

10.1007/BF01310503

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

29-43

issue

1-2

eissn

0304-8608

issn

1432-8798

journal_volume

111

pub_type

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