Abstract：
:By means of indirect immunofluorescence assay (IFA) using natural swine immune serum and hyperimmune serum from rabbits infected with porcine circovirus (PCV), a PCV antigen was detected present prior to the onset of viral and cellular DNA synthesis in nucleoli of cells of synchronized and growth stimulated infected PS cell cultures grown for more than 12 h in the presence of hydroxyurea. The number of cells containing specifically fluorescing nucleoli increased with increasing time of growth in the presence of hydroxyurea. The concomitant increase in the number of cells containing virus structural (VS) antigen in the nuclei and the increase in the amount of replicative (RF) DNA and accompanying 5 S DNA after release from the hydroxyurea block suggest that EA is involved in induction of PCV DNA replication. Primary pig kidney cell cultures persistently infected with PCV survived mock-infected control cultures for 16 passages. They had lost contact inhibition and formed cell colonies in soft agar at a ratio of 0.1 to 0.4%. Cell lines derived from agar colonies showed properties of transformed cells e.g. low requirement for serum growth factors, ability to overgrow a continuous cell layer, anchorage independence of growth. In transformed cells stimulated to growth and grown in the presence of hydroxyurea, non-structural viral antigen visible by IFA in nucleoli and VS antigen located in the cytoplasm were expressed. Contrary to virus bound nuclear VS antigen in productive infection, accumulation of cytoplasmatic VS antigen was independent of DNA synthesis and caused cell destruction, thus limiting growth of cell layers and colonies in soft agar.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Tischer I,Peters D,Pociuli Sdoi
10.1007/BF01384343subject
Has Abstractpub_date
1995-01-01 00:00:00pages
1799-816issue
10eissn
0304-8608issn
1432-8798journal_volume
140pub_type
杂志文章abstract：:The complete genome sequence (9,865 nucleotides) of a highly divergent johnsongrass mosaic virus isolate (JGMV-CNPGL) was determined using Illumina sequencing. This isolate infected 10 genotypes of gramineous plants including maize. A comparative analysis of the complete genome showed 80 % nucleotide (nt) sequence ide...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2832-9
更新日期：2016-07-01 00:00:00
abstract：:Genomic characterization of Norwalk-like human caliciviruses (NLVs) originating from outbreaks and sporadic cases of acute gastroenteritis has revealed surprisingly high levels of diversity, even in the RNA polymerase gene, which is anticipated to be highly conserved. Since information on antigenic relationship is lim...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050020
更新日期：2000-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318133
更新日期：1981-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310704
更新日期：1990-01-01 00:00:00
abstract：:Two types of transcripts, incomplete (polyadenylated virus-specific mRNAs) and complete (presumably templates for progeny virus replication) are synthesized in influenza virus infected cells (3). The synthesis of incomplete transcripts requires as a primer de novo synthesized host cell mRNAs (4). We show here that the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314609
更新日期：1981-01-01 00:00:00
abstract：:A novel Vibrio alginolyticus phage, VAP7, was isolated from seawater collected from Sanya, Hainan province, China. Whole-genome sequencing analysis revealed that phage VAP7 has a linear, double-stranded DNA genome of 144,685 bp with an average G+C content of 41.9% and a high degree of sequence similarity to Vibrio pha...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04535-4
更新日期：2020-04-01 00:00:00
abstract：:A novel strain of Japanese yam mosaic virus (JYMV-CN) was identified in a yam plant with foliar mottle symptoms in China. The complete genomic sequence of JYMV-CN was determined. Its genomic sequence of 9701 nucleotides encodes a polyprotein of 3247 amino acids. Its organization is virtually identical to that of two J...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2231-z
更新日期：2015-02-01 00:00:00
abstract：:A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314377
更新日期：1977-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0785-0
更新日期：2006-10-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-001-0792-0
更新日期：2002-06-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2999-0
更新日期：2016-11-01 00:00:00
abstract：:In the aim to study the function of the bovine herpesvirus 1 (BoHV-1) UL28 protein during the replicative cycle, we characterized a UL28 deletion mutant of BoHV-1, BoHV-1 Delta UL28. Productive growth of BoHV-1 Delta UL28 was only observed in a specifically engineered complementing cell line expressing the native UL28...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0962-8
更新日期：2003-04-01 00:00:00
abstract：:An apparently single rotavirus A strain possessing a genotype constellation of G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 abruptly emerged, caused diarrhoea in children requiring hospitalisation, and increased to reach 27 % of strains detected during the first half of 2015 in Vietnam. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2682-x
更新日期：2016-02-01 00:00:00
abstract：:BK virus (BKV)-transformed cells and BKV-induced tumors as well as in vitro derived tumor cell lines were all found to contain BKV DNA sequences when analysed by DNA-DNA reassociation kinetics. In transformed cells the number of viral genome equivalents per diploid cell genome (CG divided by VG) decreased with increas...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314838
更新日期：1981-01-01 00:00:00
abstract：:The ability of lymphocytes from patients with multiple sclerosis (MS) to produce Interferon (IFN-alpha) in response to Newcastle Disease Virus (NDV) was studied in vitro. The correlation between individual IFN-alpha titers and natural killer (NK) cell activity and the presence of HLA system antigens associated with MS...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314877
更新日期：1982-01-01 00:00:00
abstract：:Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are two closely related alphaherpesviruses. BoHV-1 causes several syndromes in cattle, including respiratory disease and sporadic cases of encephalitis, whereas BoHV-5 is responsible for meningoencephalitis in calves. Although both viruses are neurotropic, they differ...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-019-04494-5
更新日期：2020-02-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0368-x
更新日期：2004-12-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050283
更新日期：1998-01-01 00:00:00
abstract：:In this study, we calculated the relative synonymous codon usage (RSCU) value and the effective number of codons (ENC) value to carry out principal component analysis (PCA) and correlation analysis of the codon usage pattern of the phosphoprotein gene (P gene) of spring viraemia of carp virus (SVCV). The synonymous co...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2000-z
更新日期：2014-07-01 00:00:00
abstract：:Cellular uptake of fowl plague virus occurs 10-30 minutes after inoculation of chick embryo cells. The penetration of the virions is by pinocytosis (viropexis); fusion with the cellular membrane has not been observed. After pinocytosis the virions become gradually disintegrated. Budding of newly formed virions from th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317537
更新日期：1975-01-01 00:00:00
abstract：:BALB/c mice developed contralateral necrotizing retinitis following intracameral inoculation with herpes simplex virus type 1 (HSV-1). The animals showed a positive delayed-type hypersensitivity (DTH) response at 10 days postinoculation, indicating that the anterior chamber-associated immune deviation was transient af...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309535
更新日期：1993-01-01 00:00:00
abstract：:Mouse hepatitis virus 3 (MHV-3) is highly hepatotropic in sensitive mice. Temperature-sensitive mutants (ts mutants) induced by N-methyl-N'-nitrosoguanidine and 5-fluorouracil were isolated. Twelve mutants which were able to induce the formation of syncytia at 33 degrees C but not at the restrictive temperature (39.5 ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01487679
更新日期：1988-01-01 00:00:00
abstract：:Non-structural as well as VP1 recombinant proteins of foot-and-mouth disease virus (FMDV) produced in E. coli, have been used to study the specific antibody response of infected or vaccinated swine. An analysis of sera from infected pigs, using a direct ELISA, showed that polypeptide 3ABC (spanning non-structural prot...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01538822
更新日期：1994-01-01 00:00:00
abstract：:Different patterns of disease were observed among 11 chimpanzees who were inoculated intravenously with hepatitis E virus (HEV) positive fecal specimens from four different outbreaks (Nepal 1981, Uzbekistan 1981, Pakistan 1985, and Mexico 1986). Five chimpanzees had marginal or no liver enzyme elevations within 70 day...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070065
更新日期：2000-01-01 00:00:00
abstract：:Few reports are available on HCV molecular epidemiology among IDUs in Eastern Europe, and none in Montenegro. The aim of this study was to investigate the HCV genotype distribution in Montenegro among IDUs and to perform Bayesian and evolutionary analysis of the most prevalent HCV genotype circulating in this populati...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3224-5
更新日期：2017-06-01 00:00:00
abstract：:Non-infectious hemagglutinating particles of rabies virus accumulated in the fluid phase of chick embryo cell cultures at 6 days post-infection, though they were undetectable at 4 days. They were characterized as looped filaments resembling viral envelope as revealed by electron microscopy. Another form of hemagglutin...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317937
更新日期：1976-01-01 00:00:00
abstract：:Immunoblot analysis of apple latent spherical cheravirus (ALSV)-infected leaves using a polyclonal antibody against the 21 C-terminal amino acids of a 53 K/42 K movement protein (MP) showed that a protein with an Mr of 42 kDa (42KP) is the dominant form found in vivo, which could indicate that the second AUG is used a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0689-4
更新日期：2006-05-01 00:00:00
abstract：:An arracacha (Arracacia xanthorrhiza) plant collected in Brazil was found to be infected by a new virus. This viral isolate (named C17) systemically infected Nicotiana benthamiana and Apium graveolens. A polyclonal antibody was raised, and analysis of our arracacha germplasm collection showed a high infection rate of ...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-008-0289-1
更新日期：2009-01-01 00:00:00
abstract：:The hemagglutinin-esterase (HE) protein of betacoronavirus lineage A is a secondary receptor in the infection process and is involved in the emergence of new betacoronavirus genotypes with altered host specificity and tissue tropism. We previously reported a novel recombinant bovine coronavirus (BCoV) strain that was ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04840-y
更新日期：2020-12-01 00:00:00
abstract：:A xenotropic Moloney murine leukemia virus-derived recombinant retrovirus (MMuLVSVnlslacZ) has been utilized to study the mechanism of virus entry into endothelial and epithelial porcine cells. In the genome of this recombinant retrovirus, the nlslacZ reporter gene is under the transcriptional control of both LTR and ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314625
更新日期：1992-01-01 00:00:00
