Abstract：
:We have compared the RNA synthesis of alfalfa mosaic virus in complete (by RNAs 1, 2 and 3) and incomplete infections (by RNAs 1 and 2) of cowpea protoplasts. Both viral RNA polymerase activity and accumulation of viral RNA were measured. By annealing RNA in solution with 32P-labelled probes of plus and minus polarity followed by treatment with ribonucleases, we determined viral RNAs quantitatively in both single- and double-stranded RNA fractions. The accumulation of single-stranded RNA of positive polarity differed considerably between the two types of infection (250 ng vs. less than 1 ng per 10(5) protoplasts), although viral RNA polymerase activities as measured in vitro and the concentrations of minus RNA were similar. Since the method also measured fragmented RNA, this difference is probably not due to lack of protection of viral RNA by coat protein during incomplete infection. Synthesis of single-stranded plus RNA requires either RNA 3 itself or one of its gene products. We postulate that coat protein is the stringent regulator of alfalfa mosaic virus genomic expression.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Houwing CJ,van de Putte P,Jaspars EMdoi
10.1007/s007050050305subject
Has Abstractpub_date
1998-01-01 00:00:00pages
489-500issue
3eissn
0304-8608issn
1432-8798journal_volume
143pub_type
杂志文章abstract：:The present study reports the first complete genome sequence analysis of West Nile virus (WNV) strains isolated from brain samples from raptors. The results prove the circulation of closely related WNV lineage II strains in central Europe and genetic analysis revealed seven amino acid substitutions in structural (PrM3...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4056-7
更新日期：2019-01-01 00:00:00
abstract：:The E5 protein of papillomaviruses is a strongly hydrophobic membrane protein that can associate with the 16 kDa protein subunit of the vacuolar proton ATPase in endosomes and the Golgi apparatus resulting in raise of intraorganelle pH. We demonstrate that E5 of human papillomavirus type 16 (HPV16) when transfected in...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070048
更新日期：2000-01-01 00:00:00
abstract：:The sequence of prune dwarf ilarvirus (PDV) RNA-1 has been determined; it consists of 3,374 nucleotides and contains a single open reading frame of 3,168 nucleotides. The putative translation product is 1,055 amino acids in length with a calculated molecular mass of 118.9 kDa. Both the nucleic acid and the translated ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050210
更新日期：1997-01-01 00:00:00
abstract：:We determined the complete genome sequence of a South Korean (SK) isolate of Brugmansia mosaic virus (BruMV), which has recently been proposed to be a member of a new species in the genus Potyvirus. The genomic RNA of BruMV isolate SK is 9781 nucleotides in length (excluding the 3'-terminal poly (A)) and shares comple...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1693-8
更新日期：2013-09-01 00:00:00
abstract：:During the course of hominoid evolution, a new transcript variant of the GSDML (gasdermin-like protein) gene was formed by the integration of the antisense-oriented HERV-H (human endogenous retrovirus) LTR (long terminal repeat) element. To investigate regions that are critical for transcriptional regulation of the GS...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0105-y
更新日期：2008-01-01 00:00:00
abstract：:Antibody-like molecules consisting of the human CD 4 extracellular domain fused to human IgG1 heavy chain constant regions were genetically constructed and expressed in a BHK cell stable transfectant. Purified chimeric antibodies bound to HIV particles as it was shown by immuno electron microscopy, inhibited fusions o...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310503
更新日期：1990-01-01 00:00:00
abstract：:In this study, we isolated a mycobacteriophage infecting Mycobacterium smegmatis mc2155 from a soil sample collected in Shandong Province in China. This phage was named Shandong1. It is a member of the family Siphoviridae with an isometric head and a long tail. Its genome was found to be 60,618 bp long with 67.46% G +...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3534-7
更新日期：2017-12-01 00:00:00
abstract：:3Y1 rat fibroblasts transformed by adenovirus type 12 or by its E1A gene formed syncytia when cocultivated with the Friend as well as other ecotropic murine leukemia-virus-producing cells. In contrast, the parental 3Y1 cells and those transformed by other tumor viruses or by a chemical carcinogen exhibited no syncytiu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310628
更新日期：1990-01-01 00:00:00
abstract：:To elucidate changes associated with the attenuated virulence in a modified live porcine reproductive and respiratory syndrome (PRRS) vaccine (Boehringer Ingelheim Animal Health, St. Joseph, MO), derived from an American prototype ATCC virus VR-2332, nucleotide sequence of 3' genome covering open reading frames (ORFs)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050316
更新日期：1998-01-01 00:00:00
abstract：:A cross-sectional study was undertaken during 2012-2013 to determine the prevalence, strains and factors associated with rotavirus infection among under-5-year-old children hospitalized with acute diarrhea in Uganda. Rotaviruses were detected in 37 % (263/712) of the children. The most prevalent strains were G9P[8] (2...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2742-2
更新日期：2016-04-01 00:00:00
abstract：:Ljungan virus is a recently identified member of the family Picornaviridae that was isolated from bank voles in Sweden. LjV has been associated with [corrected] type 1 diabetes-like symptoms and myocarditis in bank voles (Myodes glareolus), and it has been suggested that it has zoonotic potential. Here, we show for th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1731-6
更新日期：2014-03-01 00:00:00
abstract：:The nucleotide sequences of the SH gene of 45 new mumps virus isolates derived from different parts of Europe, Canada and USA were determined. A phylogenetic tree was constructed which confirmed the existence of three major clusters. While clustering according to geographical origin was clear in some cases each of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050073
更新日期：1997-01-01 00:00:00
abstract：:The complete sequence of a narcissus virus isolated from the Netherlands (Narv-NL) was determined to be 8172 nucleotides in length with an open reading frame encoding for 2624 amino acids. Phylogenetic analysis indicates that Narv-NL is clustered with high confidence among representative members from the genus Maclura...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3741-x
更新日期：2018-05-01 00:00:00
abstract：:Mosquito-borne alphaviruses are widely distributed throughout the world, causing important human illnesses. Therefore, the development of methods to enable early diagnosis of infections by alphavirus is essential. We show here the development and evaluation of a quantitative real-time RT-PCR using genus-specific prime...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3019-0
更新日期：2016-11-01 00:00:00
abstract：:Out of 136 new phages, 80 (59%) are classified into 23 species according to morphology and physicochemical properties. Six new species are described and species beta 4, from a previous classification scheme, is renamed T1. The morphology of 36 phage species is schematically represented. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Knowledge of coronaviruses in wild carnivores is limited. This report describes coronavirus genetic diversity, species specificity and infection prevalence in three wild African carnivores. Coronavirus RNA was recovered from fresh feces from spotted hyena and silver-backed jackal, but not bat-eared fox. Analysis of se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1562-x
更新日期：2013-04-01 00:00:00
abstract：:Porcine noroviruses (NoVs) and sapoviruses (SaVs), which belong to the family Caliciviridae, have been considered potential zoonotic agents for human infection, and several cases have been reported in Asian countries. In this study, a total of 537 porcine fecal samples collected from 64 swine farms in Korea were teste...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0501-y
更新日期：2009-01-01 00:00:00
abstract：:Fowl adenoviruses (FAdVs) are widely distributed in chickens in Poland and throughout the world. FAdV infections have been reported in the United States, Australia, Europe, and the Mediterranean basin. Detection of FAdVs strains is very important from the epidemiological point of view and for monitoring disease outbre...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2635-4
更新日期：2016-01-01 00:00:00
abstract：:The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5' and 3' untranslate regions (UTR) and followed by a poly (A) tail. The putative polyproteins enco...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3147-6
更新日期：2017-03-01 00:00:00
abstract：:The disease caused by Newcastle disease virus (NDV) is a severe threat to the poultry industry worldwide. Recently, NDV has been isolated in the Antarctic region. Detailed studies on the mode of evolution of NDV strains isolated worldwide are relevant for our understanding of the evolutionary history of NDV. For this ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2434-y
更新日期：2015-08-01 00:00:00
abstract：:Molecular weights of six major polypeptides of infectious bronchitis virus (IBV) are: 1. 75,000; 2. 50,000; 3. 45,000; 4. 35,000; 5. 28,000 or 24,000, and 6. 22,000 dalton. According to the mobility of protein 5 the polypeptide patterns of IBV serotypes fall into two main categories. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315022
更新日期：1979-01-01 00:00:00
abstract：:Thirty-seven clinical isolates of coxsackievirus (CV) serotypes B-1, B-3, B-4, and B-5 were inoculated into male SJL mice. Twelve strains resulted in minor abnormalities of glucose metabolism in one or more of six infected mice (Tables 1 and 2). Sequential infection of male SJL mice with CVB-3, CVB-4, and CVB-5 result...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309826
更新日期：1985-01-01 00:00:00
abstract：:Drug resistance mutations in the Pol gene of human immunodeficiency virus 1 (HIV-1) are one of the critical factors associated with antiretroviral therapy (ART) failure in HIV-1 patients. The issue of resistance to reverse transcriptase inhibitors (RTIs) in HIV infection has not been adequately addressed in the Indian...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2760-8
更新日期：2016-05-01 00:00:00
abstract：:Human bocavirus (HBoV) has been detected primarily in children with acute lower respiratory tract disease (LRTD), but its occurrence, clinical profile, and role as a causative agent of RTD are not clear. The aim of this study was to investigate the prevalence and the potential clinical relevance of HBoV. Using molecul...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04396-6
更新日期：2019-12-01 00:00:00
abstract：:A pivotal step in the development of a consistent nomenclature for virus classification was the introduction of the virus species concept by the International Committee on Taxonomy of Viruses (ICTV) in 1991. Yet, almost two decades later, many virologists still are unable to differentiate between virus species and act...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-010-0600-9
更新日期：2010-04-01 00:00:00
abstract：:Translation of poliovirus RNA is initiated by entry of ribosomes into the nucleotide sequence (internal ribosomal entry site; IRES) within the 5'-untranslated region (5'-UTR). Efficiency of this translation initiation in rabbit reticulocyte lysates (RRL) was very low and was greatly enhanced by addition of the ribosom...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310034
更新日期：1994-01-01 00:00:00
abstract：:We undertook one of the most comprehensive studies on the replication and intraspecies transmission characteristics of low-pathogenicity avian influenza viruses in ducks, chickens and turkeys. Our results indicated that most of these isolates could replicate and be transmitted in poultry without inducing clinical dise...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0727-8
更新日期：2010-09-01 00:00:00
abstract：:Sapoviruses (SaVs) belong to the family Caliciviridae and are related to gastroenteritis viruses of humans and animals. These agents have been reported from several countries of the world and represent an important cause of economic loss. The Amazon area has a high degree of diversity of animals and plants, is located...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1904-3
更新日期：2014-05-01 00:00:00
abstract：:Complete nucleotide sequences of cytoplasmic inclusion (CI) genes of two strains of potato virus Y (PVY) were determined from six polymerase chain reaction (PCR)-amplified cDNA clones. The size of the CI genes of both ordinary (PVY-O) and necrotic strains (PVY-T13) was 1902 nucleotides, with a sequence homology of 83....
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309785
更新日期：1993-01-01 00:00:00
abstract：:Interleukin-2 dependent feline T-lymphoblastoid cells designated as MYA-1 cells were established. The cells were free from exogenous retroviruses and sensitive for replication of feline immunodeficiency virus (FIV). FIV can grow more efficiently in MYA-1 cells than in feline primary peripheral blood mononuclear cells....
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313750
更新日期：1989-01-01 00:00:00
